Abstract
The optimization of data from the indirect sandwich enzyme-linked immunosorbent assay has been commonly accomplished by linear regression analysis, even though the data are often essentially sigmoid. A new microcomputer software program (LISACRV) that uses a nonlinear regression statistical model to analyze the data from enzyme-linked immunosorbent assay titration experiments was developed.
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- Alevy Y. G., Blynn C. M. An avidin-biotin ELISA assay for the measurement of de novo human IgE synthesis in culture supernatants. J Immunol Methods. 1986 Mar 13;87(2):273–281. doi: 10.1016/0022-1759(86)90542-9. [DOI] [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
- Grandien M., Pettersson C. A., Gardner P. S., Linde A., Stanton A. Rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions. J Clin Microbiol. 1985 Nov;22(5):757–760. doi: 10.1128/jcm.22.5.757-760.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gripenberg M., Gripenberg G. Expression of antibody activity measured by ELISA. Anti-ssDNA antibody activity characterized by the shape of the dose-response curve. J Immunol Methods. 1983 Sep 16;62(3):315–323. doi: 10.1016/0022-1759(83)90175-8. [DOI] [PubMed] [Google Scholar]
- Hingley P. J., Ouldridge E. J. The use of a logistic model for the quantitative interpretation of indirect sandwich enzyme labelled immunosorbent assays (ELISA) for antibodies and antigens in foot and mouth disease. Comput Biol Med. 1985;15(3):137–152. doi: 10.1016/0010-4825(85)90027-7. [DOI] [PubMed] [Google Scholar]
- Kemeny D. M., Urbanek R., Samuel D., Richards D., Maasch H. J. The use of monoclonal and polyspecific antibodies in the IgE sandwich ELISA. J Immunol Methods. 1986 Feb 27;87(1):45–50. doi: 10.1016/0022-1759(86)90342-x. [DOI] [PubMed] [Google Scholar]
- Sutherland S. S. Comparison of enzyme-linked immunosorbent assay and complement fixation test for the detection of specific antibody in cattle vaccinated and challenged with Brucella abortus. J Clin Microbiol. 1985 Jul;22(1):44–47. doi: 10.1128/jcm.22.1.44-47.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zrein M., De Marcillac G., Van Regenmortel M. H. Quantitation of rheumatoid factors by enzyme immunoassay using biotinylated human IgG. J Immunol Methods. 1986 Mar 13;87(2):229–237. doi: 10.1016/0022-1759(86)90536-3. [DOI] [PubMed] [Google Scholar]