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. 1993 Dec;31(12):3190–3199. doi: 10.1128/jcm.31.12.3190-3199.1993

Genetic similarity and phenotypic diversity of commensal and pathogenic strains of Candida albicans isolated from the oral cavity.

J Hellstein 1, H Vawter-Hugart 1, P Fotos 1, J Schmid 1, D R Soll 1
PMCID: PMC266374  PMID: 8308110

Abstract

Colony phenotype and genetic similarity were assessed within and between groups of commensal and pathogenic strains of Candida albicans collected from the oral cavities of individuals in a single geographical locale. Thirty-eight percent of pathogenic isolates contained predominant or minor variant colony morphologies (other than smooth) when samples from the sites of infection were cultured on plates, while 16% of commensal isolates contained minor variant colony morphologies when samples from the sites of carriage were cultured. The genetic similarities of isolates within and between groups were assessed by DNA fingerprinting by using Southern blot hybridization with the fingerprinting probe Ca3 and analysis with the computer-assisted, automated Dendron system. Both the commensal and the pathogenic groups contained a major cluster of genetically similar C. albicans isolates representing 31 and 33% of the strains in the respective groups. When a combined dendrogram of both commensal and pathogenic isolates was generated, the major clusters of genetically similar isolates in each group mixed into one large cluster. Minor clusters in the individual dendrograms also mixed. These results suggest common clonal origins for commensal and pathogenic strains in the same geographical locale.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J., Srikantha T., Morrow B., Miyasaki S. H., White T. C., Agabian N., Schmid J., Soll D. R. Characterization and partial nucleotide sequence of the DNA fingerprinting probe Ca3 of Candida albicans. J Clin Microbiol. 1993 Jun;31(6):1472–1480. doi: 10.1128/jcm.31.6.1472-1480.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bedell G. W., Soll D. R. Effects of low concentrations of zinc on the growth and dimorphism of Candida albicans: evidence for zinc-resistant and -sensitive pathways for mycelium formation. Infect Immun. 1979 Oct;26(1):348–354. doi: 10.1128/iai.26.1.348-354.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hunter P. R. A critical review of typing methods for Candida albicans and their applications. Crit Rev Microbiol. 1991;17(6):417–434. doi: 10.3109/10408419109115206. [DOI] [PubMed] [Google Scholar]
  4. Kwon-Chung K. J., Hicks J. B., Lipke P. N. Evidence that Candida stellatoidea type II is a mutant of Candida albicans that does not express sucrose-inhibitable alpha-glucosidase. Infect Immun. 1990 Sep;58(9):2804–2808. doi: 10.1128/iai.58.9.2804-2808.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lee K. L., Buckley H. R., Campbell C. C. An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida Albicans. Sabouraudia. 1975 Jul;13(2):148–153. doi: 10.1080/00362177585190271. [DOI] [PubMed] [Google Scholar]
  6. Magee B. B., Magee P. T. Electrophoretic karyotypes and chromosome numbers in Candida species. J Gen Microbiol. 1987 Feb;133(2):425–430. doi: 10.1099/00221287-133-2-425. [DOI] [PubMed] [Google Scholar]
  7. McCreight M. C., Warnock D. W. Enhanced differentiation of isolates of Candida albicans using a modified resistogram method. Mykosen. 1982 Nov;25(11):589–598. doi: 10.1111/j.1439-0507.1982.tb01926.x. [DOI] [PubMed] [Google Scholar]
  8. Odds F. C., Abbott A. B. A simple system for the presumptive identification of Candida albicans and differentiation of strains within the species. Sabouraudia. 1980 Dec;18(4):301–317. [PubMed] [Google Scholar]
  9. Olivo P. D., McManus E. J., Riggsby W. S., Jones J. M. Mitochondrial DNA polymorphism in Candida albicans. J Infect Dis. 1987 Jul;156(1):214–215. doi: 10.1093/infdis/156.1.214. [DOI] [PubMed] [Google Scholar]
  10. Polonelli L., Archibusacci C., Sestito M., Morace G. Killer system: a simple method for differentiating Candida albicans strains. J Clin Microbiol. 1983 May;17(5):774–780. doi: 10.1128/jcm.17.5.774-780.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sadhu C., McEachern M. J., Rustchenko-Bulgac E. P., Schmid J., Soll D. R., Hicks J. B. Telomeric and dispersed repeat sequences in Candida yeasts and their use in strain identification. J Bacteriol. 1991 Jan;173(2):842–850. doi: 10.1128/jb.173.2.842-850.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Scherer S., Magee P. T. Genetics of Candida albicans. Microbiol Rev. 1990 Sep;54(3):226–241. doi: 10.1128/mr.54.3.226-241.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scherer S., Stevens D. A. A Candida albicans dispersed, repeated gene family and its epidemiologic applications. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1452–1456. doi: 10.1073/pnas.85.5.1452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scherer S., Stevens D. A. Application of DNA typing methods to epidemiology and taxonomy of Candida species. J Clin Microbiol. 1987 Apr;25(4):675–679. doi: 10.1128/jcm.25.4.675-679.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schmid J., Odds F. C., Wiselka M. J., Nicholson K. G., Soll D. R. Genetic similarity and maintenance of Candida albicans strains from a group of AIDS patients, demonstrated by DNA fingerprinting. J Clin Microbiol. 1992 Apr;30(4):935–941. doi: 10.1128/jcm.30.4.935-941.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schmid J., Rotman M., Reed B., Pierson C. L., Soll D. R. Genetic similarity of Candida albicans strains from vaginitis patients and their partners. J Clin Microbiol. 1993 Jan;31(1):39–46. doi: 10.1128/jcm.31.1.39-46.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schmid J., Voss E., Soll D. R. Computer-assisted methods for assessing strain relatedness in Candida albicans by fingerprinting with the moderately repetitive sequence Ca3. J Clin Microbiol. 1990 Jun;28(6):1236–1243. doi: 10.1128/jcm.28.6.1236-1243.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Soll D. R., Galask R., Schmid J., Hanna C., Mac K., Morrow B. Genetic dissimilarity of commensal strains of Candida spp. carried in different anatomical locations of the same healthy women. J Clin Microbiol. 1991 Aug;29(8):1702–1710. doi: 10.1128/jcm.29.8.1702-1710.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Soll D. R., Langtimm C. J., McDowell J., Hicks J., Galask R. High-frequency switching in Candida strains isolated from vaginitis patients. J Clin Microbiol. 1987 Sep;25(9):1611–1622. doi: 10.1128/jcm.25.9.1611-1622.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Soll D. R. Molecular biology of Candida pathogenesis. Mol Cell Biol Hum Dis Ser. 1992;1:131–172. doi: 10.1007/978-94-011-2384-6_5. [DOI] [PubMed] [Google Scholar]
  21. Soll D. R., Staebell M., Langtimm C., Pfaller M., Hicks J., Rao T. V. Multiple Candida strains in the course of a single systemic infection. J Clin Microbiol. 1988 Aug;26(8):1448–1459. doi: 10.1128/jcm.26.8.1448-1459.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Whelan W. L., Soll D. R. Mitotic recombination in Candida albicans: recessive lethal alleles linked to a gene required for methionine biosynthesis. Mol Gen Genet. 1982;187(3):477–485. doi: 10.1007/BF00332632. [DOI] [PubMed] [Google Scholar]
  23. Whelan W. L. The genetics of medically important fungi. Crit Rev Microbiol. 1987;14(2):99–170. doi: 10.3109/10408418709104437. [DOI] [PubMed] [Google Scholar]

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