Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 Dec;31(12):3344–3347. doi: 10.1128/jcm.31.12.3344-3347.1993

New method for detection of Candida albicans in human blood by polymerase chain reaction.

Y Miyakawa 1, T Mabuchi 1, Y Fukazawa 1
PMCID: PMC266430  PMID: 8308135

Abstract

We developed a new method for detection of Candida albicans cells in human blood by the polymerase chain reaction that amplifies a 125-bp region within a species-specific, multiple-copy DNA fragment. In preparing template DNA from C. albicans-containing blood, the addition of C. tropicalis cells, together with antibody which reacts with both cells, raised the sensitivity of detection of C. albicans to about 3 cells per 0.1 ml.

Full text

PDF
3344

Images in this article

3345Image
on p.3345
3346Image
on p.3346

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brawner D. L., Anderson G. L., Yuen K. Y. Serotype prevalence of Candida albicans from blood culture isolates. J Clin Microbiol. 1992 Jan;30(1):149–153. doi: 10.1128/jcm.30.1.149-153.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eisenstein B. I. The polymerase chain reaction. A new method of using molecular genetics for medical diagnosis. N Engl J Med. 1990 Jan 18;322(3):178–183. doi: 10.1056/NEJM199001183220307. [DOI] [PubMed] [Google Scholar]
  3. Fukazawa Y. Antigenic structure of Candida albicans. Immunochemical basis of the serologic specificity of the mannans in yeasts. Immunol Ser. 1989;47:37–62. [PubMed] [Google Scholar]
  4. Miyakawa Y., Kagaya K., Fukazawa Y., Soe G. Production and characterization of agglutinating monoclonal antibodies against predominant antigenic factors for Candida albicans. J Clin Microbiol. 1986 May;23(5):881–886. doi: 10.1128/jcm.23.5.881-886.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Miyakawa Y., Kuribayashi T., Kagaya K., Suzuki M., Nakase T., Fukazawa Y. Role of specific determinants in mannan of Candida albicans serotype A in adherence to human buccal epithelial cells. Infect Immun. 1992 Jun;60(6):2493–2499. doi: 10.1128/iai.60.6.2493-2499.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Miyakawa Y., Mabuchi T., Kagaya K., Fukazawa Y. Isolation and characterization of a species-specific DNA fragment for detection of Candida albicans by polymerase chain reaction. J Clin Microbiol. 1992 Apr;30(4):894–900. doi: 10.1128/jcm.30.4.894-900.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Murray P. R., Spizzo A. W., Niles A. C. Clinical comparison of the recoveries of bloodstream pathogens in Septi-Chek brain heart infusion broth with saponin, Septi-Chek tryptic soy broth, and the isolator lysis-centrifugation system. J Clin Microbiol. 1991 May;29(5):901–905. doi: 10.1128/jcm.29.5.901-905.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pfaller M. A. The use of molecular techniques for epidemiologic typing of Candida species. Curr Top Med Mycol. 1992;4:43–63. doi: 10.1007/978-1-4612-2762-5_2. [DOI] [PubMed] [Google Scholar]
  9. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  10. Scherer S., Magee P. T. Genetics of Candida albicans. Microbiol Rev. 1990 Sep;54(3):226–241. doi: 10.1128/mr.54.3.226-241.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Soll D. R. High-frequency switching in Candida albicans. Clin Microbiol Rev. 1992 Apr;5(2):183–203. doi: 10.1128/cmr.5.2.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stevens D. A., Odds F. C., Scherer S. Application of DNA typing methods to Candida albicans epidemiology and correlations with phenotype. Rev Infect Dis. 1990 Mar-Apr;12(2):258–266. doi: 10.1093/clinids/12.2.258. [DOI] [PubMed] [Google Scholar]
  13. Suzuki T., Kobayashi I., Kanbe T., Tanaka K. High frequency variation of colony morphology and chromosome reorganization in the pathogenic yeast Candida albicans. J Gen Microbiol. 1989 Feb;135(Pt 2):425–434. doi: 10.1099/00221287-135-2-425. [DOI] [PubMed] [Google Scholar]
  14. Tarrand J. J., Guillot C., Wenglar M., Jackson J., Lajeunesse J. D., Rolston K. V. Clinical comparison of the resin-containing BACTEC 26 Plus and the Isolator 10 blood culturing systems. J Clin Microbiol. 1991 Oct;29(10):2245–2249. doi: 10.1128/jcm.29.10.2245-2249.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES