Abstract
A new selective, differential plating medium to screen the common gram-negative urinary tract pathogens is described. The medium combines adonitol fermentation, phenylalanine deaminase, and beta-glucuronidase tests and allows the indole and cytochrome oxidase tests to be performed directly from the plates. High-level agreement with individual conventional tests was recorded in comparative studies with 504 cultures of gram-negative rods. There was 100% agreement, except for the Providencia spp. indole spot test (61.6% agreement). Adonitol fermentation by Providencia species could not be determined. Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa were identified with a high efficiency (100, 85.7, 83.5, and 100% agreement, respectively) without further testing. There was 96% overall agreement for the 267 infected urine samples tested.
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- EMBERLEY N. W., JAMESON J. E. A substitute for bile salts in culture media. J Gen Microbiol. 1956 Aug;15(1):198–204. doi: 10.1099/00221287-15-1-198. [DOI] [PubMed] [Google Scholar]
- Edberg S. C., Trepeta R. W. Rapid and economical identification and antimicrobial susceptibility test methodology for urinary tract pathogens. J Clin Microbiol. 1983 Dec;18(6):1287–1291. doi: 10.1128/jcm.18.6.1287-1291.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Farmer J. J., 3rd, Davis B. R., Hickman-Brenner F. W., McWhorter A., Huntley-Carter G. P., Asbury M. A., Riddle C., Wathen-Grady H. G., Elias C., Fanning G. R. Biochemical identification of new species and biogroups of Enterobacteriaceae isolated from clinical specimens. J Clin Microbiol. 1985 Jan;21(1):46–76. doi: 10.1128/jcm.21.1.46-76.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trepeta R. W., Edberg S. C. Methylumbelliferyl-beta-D-glucuronide-based medium for rapid isolation and identification of Escherichia coli. J Clin Microbiol. 1984 Feb;19(2):172–174. doi: 10.1128/jcm.19.2.172-174.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]