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. 1988 Jun;26(6):1120–1123. doi: 10.1128/jcm.26.6.1120-1123.1988

Brucella abortus 1119-3 O-chain polysaccharide to differentiate sera from B. abortus S-19-vaccinated and field-strain-infected cattle by agar gel immunodiffusion.

J W Cherwonogrodzky 1, K H Nielsen 1
PMCID: PMC266545  PMID: 3133389

Abstract

Purified Brucella abortus 1119-3 and Brucella melitensis 16M lipopolysaccharide O-chain polysaccharides were not precipitated in agar gel immunodiffusion by any of 24 sera from vaccinated cattle but were precipitated by 18 of 24 sera from infected cattle. This difference can be used to differentiate sera of cattle vaccinated with B. abortus S-19 from sera of some field-strain-infected cattle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alton G. G., Corner L. A., Plackett P. Vaccination against bovine brucellosis. Dev Biol Stand. 1984;56:643–647. [PubMed] [Google Scholar]
  2. Bundle D. R., Cherwonogrodzky J. W., Perry M. B. The structure of the lipopolysaccharide O-chain (M antigen) and polysaccharide B produced by Brucella melitensis 16M. FEBS Lett. 1987 Jun 1;216(2):261–264. doi: 10.1016/0014-5793(87)80702-0. [DOI] [PubMed] [Google Scholar]
  3. Bundle D. R., Gidney M. A., Perry M. B., Duncan J. R., Cherwonogrodzky J. W. Serological confirmation of Brucella abortus and Yersinia enterocolitica O:9 O-antigens by monoclonal antibodies. Infect Immun. 1984 Nov;46(2):389–393. doi: 10.1128/iai.46.2.389-393.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Caroff M., Bundle D. R., Perry M. B., Cherwonogrodzky J. W., Duncan J. R. Antigenic S-type lipopolysaccharide of Brucella abortus 1119-3. Infect Immun. 1984 Nov;46(2):384–388. doi: 10.1128/iai.46.2.384-388.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Caroff M., Bundle D. R., Perry M. B. Structure of the O-chain of the phenol-phase soluble cellular lipopolysaccharide of Yersinia enterocolitica serotype O:9. Eur J Biochem. 1984 Feb 15;139(1):195–200. doi: 10.1111/j.1432-1033.1984.tb07994.x. [DOI] [PubMed] [Google Scholar]
  6. Chappel R. J., Hayes J., Rogerson B. A., Shenfield L. J. The serological response of cattle to vaccines against brucellosis, as measured by the brucellosis radioimmunoassay and other tests. J Hyg (Lond) 1982 Feb;88(1):11–19. doi: 10.1017/s0022172400069849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Diaz R., Jones L. M., Leong D., Wilson J. B. Surface antigens of smooth brucellae. J Bacteriol. 1968 Oct;96(4):893–901. doi: 10.1128/jb.96.4.893-901.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones L. M., Berman D. T., Moreno E., Deyoe B. L., Gilsdorf M. J., Huber J. D., Nicoletti P. Evaluation of a radial immunodiffusion test with polysaccharide B antigen for diagnosis of bovine brucellosis. J Clin Microbiol. 1980 Dec;12(6):753–760. doi: 10.1128/jcm.12.6.753-760.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Lawman M. J., Ball D. R., Hoffmann E. M., Desjardin L. E., Boyle M. D. Production of Brucella abortus-specific protein A-reactive antibodies (IgG2) in infected and vaccinated cattle. Vet Microbiol. 1986 Jun;12(1):43–53. doi: 10.1016/0378-1135(86)90040-4. [DOI] [PubMed] [Google Scholar]
  11. Matyas Z., Fujikura T. Brucellosis as a world problem. Dev Biol Stand. 1984;56:3–20. [PubMed] [Google Scholar]
  12. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  13. Raybould T. J., Chantler S. Serological differentiation between infected and vaccinated cattle by using purified soluble antigens from Brucella abortus in a hemagglutination system. Infect Immun. 1980 Aug;29(2):435–441. doi: 10.1128/iai.29.2.435-441.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  15. Wright P. F., Kelly W. A., Gall D. E. Application of a timing protocol to the reduction of inter-plate variability in the indirect enzyme immunoassay for detection of anti-Brucella antibody. J Immunoassay. 1985;6(3):189–205. doi: 10.1080/01971528508063029. [DOI] [PubMed] [Google Scholar]

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