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. 1988 Jul;26(7):1271–1276. doi: 10.1128/jcm.26.7.1271-1276.1988

Detection of bacteria by hybridization of rRNA with DNA-latex and immunodetection of hybrids.

C A Miller 1, W L Patterson 1, P K Johnson 1, C T Swartzell 1, F Wogoman 1, J P Albarella 1, R J Carrico 1
PMCID: PMC266591  PMID: 2457597

Abstract

A novel nucleic acid hybridization assay with a DNA probe immobilized on 1.25-micron-diameter latex particles was developed. Hybridization of the immobilized probe DNA with sample rRNA was complete in 10 to 15 min. Alkaline phosphatase-labeled anti-DNA-RNA was allowed to bind to the DNA-RNA hybrids on the latex particles. Then the latex was collected on a small glass fiber filter pad, and bound alkaline phosphatase was quantitated by reflectance rate measurement. The method detected a broad range of bacterial species and had a detection limit of 500 cells per assay. The assay was used to screen urine samples for bacteriuria and had a sensitivity of 96.2% compared with conventional culture at a decision level of greater than or equal to 10(4) CFU/ml. The hybridization method could have broad application to the detection of bacteria and viruses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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