Abstract
A total of 270 frozen adenovirus isolates, obtained from January 1981 to December 1986, were grown in KB cells. DNA was successfully prepared from 248 of these isolates, and the prepared DNA was digested initially with SmaI restriction endonuclease and then analyzed by agarose gel electrophoresis. Restriction fragment patterns showed that 68 of these DNAs were either adenovirus type 3 (Ad3) or Ad7. Further analysis by digestion with BamHI showed that 24 isolates were Ad3a and 44 isolates were Ad7b. When Ad3 isolates were digested with SmaI or PstI, the restriction fragment patterns of the 24 isolates were identical. Of these 24 isolates, 20 (83%) also gave identical patterns when digested with HindIII, and the patterns of the remaining 4 isolates were different from that of the 20 but identical to each other. All Ad3 isolates were obtained in 1982, 1983, and 1986. All the 44 Ad7b isolates gave identical fragment patterns when digested with BglI or PvuII, and 43 of 44 (98%) isolates gave identical patterns when digested with SstII. We obtained at least two Ad7 isolates during each of the 6 years studied. Although considerable genome heterogeneity within adenovirus serotypes and subtypes has been reported, our population of isolates is remarkably homogeneous. This homogeneous set of genome types was obtained from diverse anatomical sites and was associated with a broad spectrum of clinical syndromes.
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