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. 1988 Oct;26(10):2006–2012. doi: 10.1128/jcm.26.10.2006-2012.1988

Characterization of Escherichia coli serotype O157:H7.

S Ratnam 1, S B March 1, R Ahmed 1, G S Bezanson 1, S Kasatiya 1
PMCID: PMC266806  PMID: 3053758

Abstract

A total of 174 strains of Escherichia coli serotype O157:H7 representing human isolates obtained from outbreaks and sporadic cases of hemorrhagic colitis, hemolytic-uremic syndrome, and nonbloody diarrheal illnesses as well as from asymptomatic carriers across Canada and the United States were examined. E. coli serotype O157:H7 possessed distinct biochemical markers, a 100% negative reaction for beta-glucuronidase and sorbitol, and a 100% positive reaction for raffinose and dulcitol; all strains otherwise were biochemically typical of E. coli. The vast majority (97%) of the strains were susceptible to commonly used antimicrobial agents. All strains produced readily detectable levels of Verotoxin; however, with polymyxin extraction, nearly 50% of the strains showed up to a 10-fold increase in the toxin level. None were found to mediate hemagglutination of human group A erythrocytes with or without D-mannose. The majority (approximately 70%) of the strains showed localized and diffuse adherence to HEp-2 cells and Henle 407 cells, and the adherence patterns were not very different from those observed among other E. coli strains. Twenty phage types were recognized, with phage types 1 and 2 accounting for 65% of the test strains. Plasmid analysis indicated three basic plasmid profiles: profile I was characterized by 68.7- and 4.2-megadalton (MDa) plasmids (62% of strains), profile II was characterized by 66.2- and 1.8-MDa plasmids (20% of strains), and profile III was characterized by a 62.5-MDa plasmid (18% of strains). A small number (19%) of the strains carried at least one additional plasmid over the basic complements, and these could be considered to constitute a miscellaneous category. None of the above-described characteristics of E. coli serotype O157:H7 could be directly correlated with one another, with the nature of infection, or with the geographical distribution of strains.

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Selected References

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  1. Ahmed R., Bopp C., Borczyk A., Kasatiya S. Phage-typing scheme for Escherichia coli O157:H7. J Infect Dis. 1987 Apr;155(4):806–809. doi: 10.1093/infdis/155.4.806. [DOI] [PubMed] [Google Scholar]
  2. Bopp C. A., Greene K. D., Downes F. P., Sowers E. G., Wells J. G., Wachsmuth I. K. Unusual verotoxin-producing Escherichia coli associated with hemorrhagic colitis. J Clin Microbiol. 1987 Aug;25(8):1486–1489. doi: 10.1128/jcm.25.8.1486-1489.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Borczyk A. A., Karmali M. A., Lior H., Duncan L. M. Bovine reservoir for verotoxin-producing Escherichia coli O157:H7. Lancet. 1987 Jan 10;1(8524):98–98. doi: 10.1016/s0140-6736(87)91928-3. [DOI] [PubMed] [Google Scholar]
  4. Cahoon F. E., Thompson J. S. Frequency of Escherichia coli O157:H7 isolation from stool specimens. Can J Microbiol. 1987 Oct;33(10):914–915. doi: 10.1139/m87-158. [DOI] [PubMed] [Google Scholar]
  5. Carter A. O., Borczyk A. A., Carlson J. A., Harvey B., Hockin J. C., Karmali M. A., Krishnan C., Korn D. A., Lior H. A severe outbreak of Escherichia coli O157:H7--associated hemorrhagic colitis in a nursing home. N Engl J Med. 1987 Dec 10;317(24):1496–1500. doi: 10.1056/NEJM198712103172403. [DOI] [PubMed] [Google Scholar]
  6. Doyle M. P., Schoeni J. L. Isolation of Escherichia coli O157:H7 from retail fresh meats and poultry. Appl Environ Microbiol. 1987 Oct;53(10):2394–2396. doi: 10.1128/aem.53.10.2394-2396.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Doyle M. P., Schoeni J. L. Survival and growth characteristics of Escherichia coli associated with hemorrhagic colitis. Appl Environ Microbiol. 1984 Oct;48(4):855–856. doi: 10.1128/aem.48.4.855-856.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Evans D. J., Jr, Evans D. G., Young L. S., Pitt J. Hemagglutination typing of Escherichia coli: definition of seven hemagglutination types. J Clin Microbiol. 1980 Aug;12(2):235–242. doi: 10.1128/jcm.12.2.235-242.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Farmer J. J., 3rd, Davis B. R. H7 antiserum-sorbitol fermentation medium: a single tube screening medium for detecting Escherichia coli O157:H7 associated with hemorrhagic colitis. J Clin Microbiol. 1985 Oct;22(4):620–625. doi: 10.1128/jcm.22.4.620-625.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Feng P. C., Hartman P. A. Fluorogenic assays for immediate confirmation of Escherichia coli. Appl Environ Microbiol. 1982 Jun;43(6):1320–1329. doi: 10.1128/aem.43.6.1320-1329.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Francis D. H., Collins J. E., Duimstra J. R. Infection of gnotobiotic pigs with an Escherichia coli O157:H7 strain associated with an outbreak of hemorrhagic colitis. Infect Immun. 1986 Mar;51(3):953–956. doi: 10.1128/iai.51.3.953-956.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gransden W. R., Damm M. A., Anderson J. D., Carter J. E., Lior H. Further evidence associating hemolytic uremic syndrome with infection by Verotoxin-producing Escherichia coli O157:H7. J Infect Dis. 1986 Sep;154(3):522–524. doi: 10.1093/infdis/154.3.522. [DOI] [PubMed] [Google Scholar]
  13. Johnson W. M., Lior H., Bezanson G. S. Cytotoxic Escherichia coli O157:H7 associated with haemorrhagic colitis in Canada. Lancet. 1983 Jan 1;1(8314-5):76–76. doi: 10.1016/s0140-6736(83)91616-1. [DOI] [PubMed] [Google Scholar]
  14. Karch H., Heesemann J., Laufs R., O'Brien A. D., Tacket C. O., Levine M. M. A plasmid of enterohemorrhagic Escherichia coli O157:H7 is required for expression of a new fimbrial antigen and for adhesion to epithelial cells. Infect Immun. 1987 Feb;55(2):455–461. doi: 10.1128/iai.55.2.455-461.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Karmali M. A., Petric M., Lim C., Cheung R., Arbus G. S. Sensitive method for detecting low numbers of verotoxin-producing Escherichia coli in mixed cultures by use of colony sweeps and polymyxin extraction of verotoxin. J Clin Microbiol. 1985 Oct;22(4):614–619. doi: 10.1128/jcm.22.4.614-619.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Karmali M. A., Petric M., Lim C., Fleming P. C., Arbus G. S., Lior H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. J Infect Dis. 1985 May;151(5):775–782. doi: 10.1093/infdis/151.5.775. [DOI] [PubMed] [Google Scholar]
  17. Krishnan C., Fitzgerald V. A., Dakin S. J., Behme R. J. Laboratory investigation of outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7. J Clin Microbiol. 1987 Jun;25(6):1043–1047. doi: 10.1128/jcm.25.6.1043-1047.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Levine M. M. Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J Infect Dis. 1987 Mar;155(3):377–389. doi: 10.1093/infdis/155.3.377. [DOI] [PubMed] [Google Scholar]
  19. Levine M. M., Xu J. G., Kaper J. B., Lior H., Prado V., Tall B., Nataro J., Karch H., Wachsmuth K. A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome. J Infect Dis. 1987 Jul;156(1):175–182. doi: 10.1093/infdis/156.1.175. [DOI] [PubMed] [Google Scholar]
  20. March S. B., Ratnam S. Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J Clin Microbiol. 1986 May;23(5):869–872. doi: 10.1128/jcm.23.5.869-872.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Marques L. R., Moore M. A., Wells J. G., Wachsmuth I. K., O'Brien A. D. Production of Shiga-like toxin by Escherichia coli. J Infect Dis. 1986 Aug;154(2):338–341. doi: 10.1093/infdis/154.2.338. [DOI] [PubMed] [Google Scholar]
  22. Mathewson J. J., Johnson P. C., DuPont H. L., Morgan D. R., Thornton S. A., Wood L. V., Ericsson C. D. A newly recognized cause of travelers' diarrhea: enteroadherent Escherichia coli. J Infect Dis. 1985 Mar;151(3):471–475. doi: 10.1093/infdis/151.3.471. [DOI] [PubMed] [Google Scholar]
  23. Minami J., Okabe A., Hayashi H. Enzymic detection of adhesion of enteropathogenic Escherichia coli to HEp-2 cells. Microbiol Immunol. 1987;31(9):851–858. doi: 10.1111/j.1348-0421.1987.tb03146.x. [DOI] [PubMed] [Google Scholar]
  24. Pai C. H., Gordon R., Sims H. V., Bryan L. E. Sporadic cases of hemorrhagic colitis associated with Escherichia coli O157:H7. Clinical, epidemiologic, and bacteriologic features. Ann Intern Med. 1984 Dec;101(6):738–742. doi: 10.7326/0003-4819-101-6-738. [DOI] [PubMed] [Google Scholar]
  25. Pai C. H., Kelly J. K., Meyers G. L. Experimental infection of infant rabbits with verotoxin-producing Escherichia coli. Infect Immun. 1986 Jan;51(1):16–23. doi: 10.1128/iai.51.1.16-23.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ratnam S., March S. B. Sporadic occurrence of hemorrhagic colitis associated with Escherichia coli O157:H7 in Newfoundland. CMAJ. 1986 Jan 1;134(1):43-5, 49. [PMC free article] [PubMed] [Google Scholar]
  27. Ratnam S., March S. B., Sprague W. D., Severs D., Sullivan R. M. 3535529Are humans a source of Escherichia coli O157:H7, the agent of hemorrhagic colitis. N Engl J Med. 1986 Dec 18;315(25):1612–1613. doi: 10.1056/NEJM198612183152513. [DOI] [PubMed] [Google Scholar]
  28. Remis R. S., MacDonald K. L., Riley L. W., Puhr N. D., Wells J. G., Davis B. R., Blake P. A., Cohen M. L. Sporadic cases of hemorrhagic colitis associated with Escherichia coli O157:H7. Ann Intern Med. 1984 Nov;101(5):624–626. doi: 10.7326/0003-4819-101-5-624. [DOI] [PubMed] [Google Scholar]
  29. Riley L. W., Remis R. S., Helgerson S. D., McGee H. B., Wells J. G., Davis B. R., Hebert R. J., Olcott E. S., Johnson L. M., Hargrett N. T. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med. 1983 Mar 24;308(12):681–685. doi: 10.1056/NEJM198303243081203. [DOI] [PubMed] [Google Scholar]
  30. Ryan C. A., Tauxe R. V., Hosek G. W., Wells J. G., Stoesz P. A., McFadden H. W., Jr, Smith P. W., Wright G. F., Blake P. A. Escherichia coli O157:H7 diarrhea in a nursing home: clinical, epidemiological, and pathological findings. J Infect Dis. 1986 Oct;154(4):631–638. doi: 10.1093/infdis/154.4.631. [DOI] [PubMed] [Google Scholar]
  31. Scaletsky I. C., Silva M. L., Toledo M. R., Davis B. R., Blake P. A., Trabulsi L. R. Correlation between adherence to HeLa cells and serogroups, serotypes, and bioserotypes of Escherichia coli. Infect Immun. 1985 Sep;49(3):528–532. doi: 10.1128/iai.49.3.528-532.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Scaletsky I. C., Silva M. L., Trabulsi L. R. Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun. 1984 Aug;45(2):534–536. doi: 10.1128/iai.45.2.534-536.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sherman P., Soni R., Petric M., Karmali M. Surface properties of the Vero cytotoxin-producing Escherichia coli O157:H7. Infect Immun. 1987 Aug;55(8):1824–1829. doi: 10.1128/iai.55.8.1824-1829.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Smith H. R., Rowe B., Gross R. J., Fry N. K., Scotland S. M. Haemorrhagic colitis and Vero-cytotoxin-producing Escherichia coli in England and Wales. Lancet. 1987 May 9;1(8541):1062–1065. doi: 10.1016/s0140-6736(87)90485-5. [DOI] [PubMed] [Google Scholar]
  35. Spika J. S., Parsons J. E., Nordenberg D., Wells J. G., Gunn R. A., Blake P. A. Hemolytic uremic syndrome and diarrhea associated with Escherichia coli O157:H7 in a day care center. J Pediatr. 1986 Aug;109(2):287–291. doi: 10.1016/s0022-3476(86)80386-9. [DOI] [PubMed] [Google Scholar]
  36. Tzipori S., Karch H., Wachsmuth K. I., Robins-Browne R. M., O'Brien A. D., Lior H., Cohen M. L., Smithers J., Levine M. M. Role of a 60-megadalton plasmid and Shiga-like toxins in the pathogenesis of infection caused by enterohemorrhagic Escherichia coli O157:H7 in gnotobiotic piglets. Infect Immun. 1987 Dec;55(12):3117–3125. doi: 10.1128/iai.55.12.3117-3125.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Wells J. G., Davis B. R., Wachsmuth I. K., Riley L. W., Remis R. S., Sokolow R., Morris G. K. Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J Clin Microbiol. 1983 Sep;18(3):512–520. doi: 10.1128/jcm.18.3.512-520.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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