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. 1988 Oct;26(10):2101–2104. doi: 10.1128/jcm.26.10.2101-2104.1988

Immunoglobulin A antibodies to Trypanosoma cruzi antigens in digestive forms of Chagas' disease.

K S Primavera 1, S Hoshino-Shimizu 1, E S Umezawa 1, B A Peres 1, D A Manigot 1, M E Camargo 1
PMCID: PMC266824  PMID: 3141458

Abstract

In an attempt to find a serological marker for the diagnosis of chronic digestive forms of Chagas' disease, we compared amastigote and trypomastigote antigens obtained from immunosuppressed mice infected with Trypanosoma cruzi (Y strain) with conventional epimastigote antigens to search for immunoglobulin A (IgA) antibodies. A total of 255 serum samples from patients with acute and chronic (indeterminate, digestive, and cardiac) forms of Chagas' disease and with nonchagasic diseases and from healthy individuals were studied. Amastigote antigens proved to be the most adequate for our purpose, since IgA antibodies could be detected in 23 of 25 serum samples from patients with digestive forms, with relative indices of sensitivity, specificity, and efficiency of 0.920, 0.911, and 0.912, respectively. These antigens also showed high reactivity with IgA antibodies, with a geometric mean titer of 16,635 (12.7 log2). IgA antibodies were detected in 16 of 28 serum samples from patients with the acute form as well, but this clinical form is easily distinguished from the chronic form by the demonstration of IgM antibodies. Poor results were seen with trypomastigote and epimastigote antigens. The finding of IgA antibodies in about 20% of indeterminate forms and 20% of cardiac forms, although in low titers, requires further investigation to ascertain their role as an early signal of gastrointestinal lesions. In addition, the amastigote antigens described here seem more convenient for use in endemic areas than those obtained from cell cultures because of their lower cost.

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Selected References

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  1. Abrahamsohn I. A., Katzin A. M., Milder R. V. A method for isolating Trypanosoma cruzi amastigotes from spleen and liver using two-step discontinuous gradient centrifugation. J Parasitol. 1983 Apr;69(2):437–439. [PubMed] [Google Scholar]
  2. Araujo F. G., Guptill D. Use of antigen preparations of the amastigote stage of Trypanosoma cruzi in the serology of Chagas' disease. Am J Trop Med Hyg. 1984 May;33(3):362–371. doi: 10.4269/ajtmh.1984.33.362. [DOI] [PubMed] [Google Scholar]
  3. Biagioni L., Souza M. J., Chamma L. G., Mendes R. P., Marques S. A., Mota N. G., Franco M. Serology of paracoccidioidomycosis. II. Correlation between class-specific antibodies and clinical forms of the disease. Trans R Soc Trop Med Hyg. 1984;78(5):617–621. doi: 10.1016/0035-9203(84)90220-7. [DOI] [PubMed] [Google Scholar]
  4. Camargo M. E. Fluorescent antibody test for the serodiagnosis of American trypanosomiasis. Technical modification employing preserved culture forms of Trypanosoma cruzi in a slide test. Rev Inst Med Trop Sao Paulo. 1966 Sep-Oct;8(5):227–235. [PubMed] [Google Scholar]
  5. Camargo M. E., Hoshino-Shimizu S., Umezawa E. S. Further evaluation of the "I.M.T.-Chagas flocculation test". A comparison with complement fixation, hemagglutination and immunofluorescence tests. Rev Inst Med Trop Sao Paulo. 1975 Jul-Aug;17(4):230–235. [PubMed] [Google Scholar]
  6. Castro C., de Rezende J. M., Camargo M., Prata A., Macêdo V. Prevalência de esofagopatia chagásica no município de Mambaí, Goiás-Brasil. Rev Soc Bras Med Trop. 1987 Jan-Mar;20(1):13–17. doi: 10.1590/s0037-86821987000100003. [DOI] [PubMed] [Google Scholar]
  7. Cerisola J. A., Alvarez M., Bock M., Wegner D. A comparison of a new antigen from amastigotes of Trypanosoma cruzi and an antigen from epimastigotes for the diagnosis of Chagas' disease by the indirect immunofluorescence test. Rev Inst Med Trop Sao Paulo. 1971 May-Jun;13(3):162–166. [PubMed] [Google Scholar]
  8. Corsini A. C., Braz R., Ciampi D. B., Zucato M. R. Resistance to Trypanosoma cruzi infection in relation to the timing of IgG humoral response. Z Parasitenkd. 1982;68(1):15–25. doi: 10.1007/BF00926653. [DOI] [PubMed] [Google Scholar]
  9. Cossio P. M., Casanova M. B., González Cappa S., Fernández L., Arana R. M. El posible uso de amastigotes tisulares de T. cruzi para pruebas serológicas de inmunofluorescencia. Medicina (B Aires) 1983;43(6 Pt 1):737–738. [PubMed] [Google Scholar]
  10. Guimarães M. C., Ferreira A. W., de Carvalho M. B., Celeste B. J., Cucé L. C., Belda Júnior W. Anti-leishmania IgA immunoenzymatic assay in mucocutaneous leishmaniasis (preliminary report). Rev Inst Med Trop Sao Paulo. 1984 Nov-Dec;26(6):353–356. doi: 10.1590/s0036-46651984000600011. [DOI] [PubMed] [Google Scholar]
  11. Gusmão R. D., Rezende J. M., Rassi A., Gam A. A., Neva F. A. Antibody levels to Trypanosoma cruzi in infected patients with and without evidence of chronic Chagas' disease. Am J Trop Med Hyg. 1982 May;31(3 Pt 1):452–458. doi: 10.4269/ajtmh.1982.31.452. [DOI] [PubMed] [Google Scholar]
  12. Hoshino-Shimizu S., Camargo M. E., Nagasse T. K. A stable polysaccharide-hemagglutination reagent for the diagnosis of acute or recent Trypanosoma cruzi infections. Rev Inst Med Trop Sao Paulo. 1978 Jul-Aug;20(4):208–212. [PubMed] [Google Scholar]
  13. Kloetzel J., Camargo M. E., Giovannini V. L. Antigenic differences among epimastigotes, amastigotes and trypomastigotes of Trypanosoma cruzi. J Protozool. 1975 May;22(2):259–261. doi: 10.1111/j.1550-7408.1975.tb05862.x. [DOI] [PubMed] [Google Scholar]
  14. Krettli A. U., Brener Z. Resistance against Trypanosoma cruzi associated to anti-living trypomastigote antibodies. J Immunol. 1982 May;128(5):2009–2012. [PubMed] [Google Scholar]
  15. Medrado-Faria M. de A., Yasuda M. A., Araujo M. J., Lancarotte I., Catapano E. A., Ruiz Neto P. P. Formas clínicas da doença de Chagas na Grande São Paulo. Arq Bras Cardiol. 1982 Feb;38(2):99–109. [PubMed] [Google Scholar]
  16. Mota E., Todd C. W., Maguire J. H., Portugal D., Santana O., Ribeiro Filho R., Sherlock I. A. Megaesophagus and seroreactivity to Trypanosoma cruzi in a rural community in northeast Brazil. Am J Trop Med Hyg. 1984 Sep;33(5):820–826. doi: 10.4269/ajtmh.1984.33.820. [DOI] [PubMed] [Google Scholar]
  17. Partanen P., Seppänen H., Suni J., Vaheri A. Selective reactivity of antibodies to human immunoglobulins G, M, and A with rubella virus proteins. J Clin Microbiol. 1985 May;21(5):800–802. doi: 10.1128/jcm.21.5.800-802.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pinon J. M., Thoannes H., Pouletty P. H., Poirriez J., Damiens J., Pelletier P. Detection of IgA specific for toxoplasmosis in serum and cerebrospinal fluid using a non-enzymatic IgA-capture assay. Diagn Immunol. 1986;4(5):223–227. [PubMed] [Google Scholar]
  19. Shaw J. J., Lainson R. Leishmaniasis in Brazil. XIV. Leishmanial and trypanosomal IgA antibody in patients with leishmaniasis and Chagas's disease. Trans R Soc Trop Med Hyg. 1981;75(2):254–257. doi: 10.1016/0035-9203(81)90329-1. [DOI] [PubMed] [Google Scholar]
  20. Sá Ferreira J. A., Galvão-Castro B., Macedo W., Castro C. Immunoglobulins and other serological parameters in Chagas' disease: evidence for increased IgA levels in the chronic digestive form. Clin Exp Immunol. 1983 May;52(2):266–270. [PMC free article] [PubMed] [Google Scholar]
  21. Voller A., Draper C., Bidwell D. E., Bartlett A. Microplate enzyme-linked immunosorbent assay for chagas' disease. Lancet. 1975 Feb 22;1(7904):426–428. doi: 10.1016/s0140-6736(75)91492-0. [DOI] [PubMed] [Google Scholar]

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