Abstract
The sensitivities, specificities, and positive and negative predictive values of the Cambridge BioScience Corp. (Worcester, Mass.) human immunodeficiency virus latex agglutination assay were compared by using three different blood preparations. By using the manufacturer's standard test method with diluted sera, the sensitivity of latex agglutination was 100%, the specificity was 99.58%, and the positive and negative predictive values were 99.26 and 100%, respectively. Use of diluted whole blood or undiluted whole blood did not significantly affect the sensitivity (mean, 99.72%), specificity (mean, 99.47%), positive predictive value (mean, 99.07%), or negative predictive value (mean, 99.89%). The latex agglutination assay is a simple, rapid assay for the detection of human immunodeficiency virus that would be useful in Third World countries or other areas where enzyme-linked immunosorbent assays are not available or cannot be used.
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- Barré-Sinoussi F., Chermann J. C., Rey F., Nugeyre M. T., Chamaret S., Gruest J., Dauguet C., Axler-Blin C., Vézinet-Brun F., Rouzioux C. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science. 1983 May 20;220(4599):868–871. doi: 10.1126/science.6189183. [DOI] [PubMed] [Google Scholar]
- Carlson J. R., Mertens S. C., Yee J. L., Gardner M. B., Watson-Williams E. J., Ghrayeb J., Jennings M. B., Biggar R. J. Rapid, easy, and economical screening test for antibodies to human immunodeficiency virus. Lancet. 1987 Feb 14;1(8529):361–362. doi: 10.1016/s0140-6736(87)91730-2. [DOI] [PubMed] [Google Scholar]
- Gallo R. C., Sarin P. S., Gelmann E. P., Robert-Guroff M., Richardson E., Kalyanaraman V. S., Mann D., Sidhu G. D., Stahl R. E., Zolla-Pazner S. Isolation of human T-cell leukemia virus in acquired immune deficiency syndrome (AIDS). Science. 1983 May 20;220(4599):865–867. doi: 10.1126/science.6601823. [DOI] [PubMed] [Google Scholar]
- Ivo dos Santos J., Galvao-Castro B., Mello D. C., Pereira H. G., Pereira M. S. Dot enzyme immunoassay. A simple, cheap and stable test for antibody to human immunodeficiency virus (HIV). J Immunol Methods. 1987 May 20;99(2):191–194. doi: 10.1016/0022-1759(87)90126-8. [DOI] [PubMed] [Google Scholar]
- Mann J. M., Francis H., Quinn T. C., Bila K., Asila P. K., Bosenge N., Nzilambi N., Jansegers L., Piot P., Ruti K. HIV seroprevalence among hospital workers in Kinshasa, Zaire. Lack of association with occupational exposure. JAMA. 1986 Dec 12;256(22):3099–3102. [PubMed] [Google Scholar]
- Petricciani J. C. Licensed tests for antibody to human T-lymphotropic virus type III. Sensitivity and specificity. Ann Intern Med. 1985 Nov;103(5):726–729. doi: 10.7326/0003-4819-103-5-726. [DOI] [PubMed] [Google Scholar]
- Quinn T. C., Mann J. M., Curran J. W., Piot P. AIDS in Africa: an epidemiologic paradigm. Science. 1986 Nov 21;234(4779):955–963. doi: 10.1126/science.3022379. [DOI] [PubMed] [Google Scholar]
- Quinn T. C., Riggin C. H., Kline R. L., Francis H., Mulanga K., Sension M. G., Fauci A. S. Rapid latex agglutination assay using recombinant envelope polypeptide for the detection of antibody to the HIV. JAMA. 1988 Jul 22;260(4):510–513. [PubMed] [Google Scholar]
- Ratner L., Haseltine W., Patarca R., Livak K. J., Starcich B., Josephs S. F., Doran E. R., Rafalski J. A., Whitehorn E. A., Baumeister K. Complete nucleotide sequence of the AIDS virus, HTLV-III. Nature. 1985 Jan 24;313(6000):277–284. doi: 10.1038/313277a0. [DOI] [PubMed] [Google Scholar]
- Riggin C. H., Beltz G. A., Hung C. H., Thorn R. M., Marciani D. J. Detection of antibodies to human immunodeficiency virus by latex agglutination with recombinant antigen. J Clin Microbiol. 1987 Sep;25(9):1772–1773. doi: 10.1128/jcm.25.9.1772-1773.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thorn R. M., Beltz G. A., Hung C. H., Fallis B. F., Winkle S., Cheng K. L., Marciani D. J. Enzyme immunoassay using a novel recombinant polypeptide to detect human immunodeficiency virus env antibody. J Clin Microbiol. 1987 Jul;25(7):1207–1212. doi: 10.1128/jcm.25.7.1207-1212.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]