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. 1988 Dec;26(12):2474–2479. doi: 10.1128/jcm.26.12.2474-2479.1988

Recognition of mycobacterial antigens by sera from patients with leprosy.

F Vega-Lopez 1, N G Stoker 1, M F Locniskar 1, H M Dockrell 1, K A Grant 1, K P McAdam 1
PMCID: PMC266928  PMID: 3068245

Abstract

Mycobacterium leprae sonic extracts prepared from armadillo-derived bacteria were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting) procedures and probed with serum or plasma samples from 20 patients with lepromatous leprosy and 14 healthy endemic controls. Five proteins of 33, 25, 18, 15, and 12 kilodaltons (kDa) were frequently recognized; the 33- and 15-kDa proteins were, respectively, recognized with high intensity by 16 and 13 of the 20 samples from patients with leprosy, whereas only one healthy donor had antibodies that recognized the 15-kDa protein. By the use of M. leprae-specific murine monoclonal antibodies it was demonstrated that the 33-, 25-, and 15-kDa antigens were different from those bound by the available murine monoclonal antibodies. The 18- and 12-kDa proteins detected had molecular masses similar to those detected by the corresponding murine monoclonal antibodies. The serum and plasma samples from patients with leprosy were also used to probe Western blots of a soluble extract of M. tuberculosis. They recognized, among others, antigens with molecular weights similar to those detected in the M. leprae antigenic preparations, although with less intensity and at a lower frequency.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Britton W. J., Garsia R. J., Hellqvist L., Watson J. D., Basten A. The characterization and immunoreactivity of a 70 kD protein common to Mycobacterium leprae and Mycobacterium bovis (BCG). Lepr Rev. 1986 Dec;57 (Suppl 2):67–75. [PubMed] [Google Scholar]
  2. Britton W. J., Hellqvist L., Basten A., Raison R. L. Mycobacterium leprae antigens involved in human immune responses. I. Identification of four antigens by monoclonal antibodies. J Immunol. 1985 Dec;135(6):4171–4177. [PubMed] [Google Scholar]
  3. Britton W. J., Hellqvist L., Garsia R. J., Basten A. Antigens of Mycobacterium leprae identified by immunoprecipitation with sera from leprosy and tuberculosis patients. Clin Exp Immunol. 1988 Mar;71(3):394–398. [PMC free article] [PubMed] [Google Scholar]
  4. Britton W. J., Hellqvist L., Garsia R. J., Basten A. Dominant cell wall proteins of Mycobacterium leprae recognized by monoclonal antibodies. Clin Exp Immunol. 1987 Jan;67(1):31–42. [PMC free article] [PubMed] [Google Scholar]
  5. Buchanan T. M., Nomaguchi H., Anderson D. C., Young R. A., Gillis T. P., Britton W. J., Ivanyi J., Kolk A. H., Closs O., Bloom B. R. Characterization of antibody-reactive epitopes on the 65-kilodalton protein of Mycobacterium leprae. Infect Immun. 1987 Apr;55(4):1000–1003. doi: 10.1128/iai.55.4.1000-1003.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chakrabarty A. K., Maire M. A., Lambert P. H. SDS-PAGE analysis of M. leprae protein antigens reacting with antibodies from sera from lepromatous patients and infected armadillos. Clin Exp Immunol. 1982 Sep;49(3):523–531. [PMC free article] [PubMed] [Google Scholar]
  7. Closs O., Mshana R. N., Harboe M. Antigenic analysis of Mycobacterium leprae. Scand J Immunol. 1979 Mar;9(3):297–302. doi: 10.1111/j.1365-3083.1979.tb02735.x. [DOI] [PubMed] [Google Scholar]
  8. Ehrenberg J. P., Gebre N. Analysis of the antigenic profile of Mycobacterium leprae: cross-reactive and unique specificities of human and rabbit antibodies. Scand J Immunol. 1987 Dec;26(6):673–681. doi: 10.1111/j.1365-3083.1987.tb02303.x. [DOI] [PubMed] [Google Scholar]
  9. Hunter S. W., Gaylord H., Brennan P. J. Structure and antigenicity of the phosphorylated lipopolysaccharide antigens from the leprosy and tubercle bacilli. J Biol Chem. 1986 Sep 15;261(26):12345–12351. [PubMed] [Google Scholar]
  10. Ivanyi J., Sinha S., Aston R., Cussell D., Keen M., Sengupta U. Definition of species specific and cross-reactive antigenic determinants of Mycobacterium leprae using monoclonal antibodies. Clin Exp Immunol. 1983 Jun;52(3):528–536. [PMC free article] [PubMed] [Google Scholar]
  11. Klatser P. R., De Wit M. Y., Kolk A. H. An ELISA-inhibition test using monoclonal antibody for the serology of leprosy. Clin Exp Immunol. 1985 Dec;62(3):468–473. [PMC free article] [PubMed] [Google Scholar]
  12. Klatser P. R., van Rens M. M., Eggelte T. A. Immunochemical characterization of Mycobacterium leprae antigens by the SDS-polyacrylamide gel electrophoresis immunoperoxidase technique (SGIP) using patients' sera. Clin Exp Immunol. 1984 Jun;56(3):537–544. [PMC free article] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Lamb J. R., Ivanyi J., Rees A. D., Rothbard J. B., Howland K., Young R. A., Young D. B. Mapping of T cell epitopes using recombinant antigens and synthetic peptides. EMBO J. 1987 May;6(5):1245–1249. doi: 10.1002/j.1460-2075.1987.tb02360.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ridley D. S., Jopling W. H. Classification of leprosy according to immunity. A five-group system. Int J Lepr Other Mycobact Dis. 1966 Jul-Sep;34(3):255–273. [PubMed] [Google Scholar]
  16. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Young D. B., Buchanan T. M. A serological test for leprosy with a glycolipid specific for Mycobacterium leprae. Science. 1983 Sep 9;221(4615):1057–1059. doi: 10.1126/science.6348948. [DOI] [PubMed] [Google Scholar]
  18. Young D. B., Fohn M. J., Khanolkar S. R., Buchanan T. M. Monoclonal antibodies to a 28,000 mol. wt protein antigen of Mycobacterium leprae. Clin Exp Immunol. 1985 Jun;60(3):546–552. [PMC free article] [PubMed] [Google Scholar]
  19. Young R. A., Mehra V., Sweetser D., Buchanan T., Clark-Curtiss J., Davis R. W., Bloom B. R. Genes for the major protein antigens of the leprosy parasite Mycobacterium leprae. Nature. 1985 Aug 1;316(6027):450–452. doi: 10.1038/316450a0. [DOI] [PubMed] [Google Scholar]

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