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. 1989 Oct;27(10):2204–2207. doi: 10.1128/jcm.27.10.2204-2207.1989

Establishment of titration system for human herpesvirus 6 and evaluation of neutralizing antibody response to the virus.

H Asada 1, S Yalcin 1, K Balachandra 1, K Higashi 1, K Yamanishi 1
PMCID: PMC266994  PMID: 2555389

Abstract

The susceptibilities of seven T-cell lines to human herpesvirus 6 (HHV-6) infection were examined. MT-4 cells were the most susceptible of these lines to infection with this virus. Therefore, chemically adhered MT-4 cell monolayers were used for infectious HHV-6 assay by indirect immunofluorescent-antibody (IFA) staining. When cell monolayers were fixed 30 to 45 h postinfection, the foci stained with IFA were easy to count and a linear relationship was observed between the number of foci and the virus concentration. MT-4 cell monolayers were also used for a focus reduction neutralizing-antibody test. In this test, sera from patients in the convalescent stage of exanthem subitum all showed significant neutralizing activity (1:80 to 1:320), whereas sera from patients in the acute stage of disease showed no detectable neutralizing activity. The titers of neutralizing antibody correlated well with the levels of anti-HHV-6 antibodies detected by IFA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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