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. 1989 Dec;27(12):2842–2845. doi: 10.1128/jcm.27.12.2842-2845.1989

Detection of the 43,000-molecular-weight glycoprotein in sera of patients with paracoccidioidomycosis.

M J Mendes-Giannini 1, J P Bueno 1, M A Shikanai-Yasuda 1, A W Ferreira 1, A Masuda 1
PMCID: PMC267138  PMID: 2592544

Abstract

The 43,000-molecular-weight (43K) soluble glycoprotein was detected in sera of patients with paracoccidioidomycosis by the immunoblot technique by using as the probe rabbit monospecific antisera to this fraction. The 43K antigen was present before treatment in sera of patients with the acute (juvenile) form; it started to disappear from circulation after 10 months of chemotherapy, and it was undetectable after 2 years of treatment. In the chronic cases, the 43K antigen was detected in patients without treatment, and it was absent in the healed cases. The detection of the 43K protein specific to Paracoccidioides brasiliensis may be important for its diagnostic value as well as for modulation of the host immune response.

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Selected References

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  1. Azuma I., Kanetsuna F., Tanaka Y., Yamamura Y., Carbonell L. M. Chemical and immunological properties of galactomannans obtained from Histoplasma duboisii, Histoplasma capsulatum, Paracoccidioides brasiliensis and Blasomyces dermatitidis. Mycopathol Mycol Appl. 1974 Oct 15;54(1):111–125. doi: 10.1007/BF02055979. [DOI] [PubMed] [Google Scholar]
  2. Ellsworth J. H., Reiss E., Bradley R. L., Chmel H., Armstrong D. Comparative serological and cutaneous reactivity of candidal cytoplasmic proteins and mannan separated by affinity for concanavalin A. J Clin Microbiol. 1977 Jan;5(1):91–99. doi: 10.1128/jcm.5.1.91-99.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Galgiani J. N., Dugger K. O., Ito J. I., Wieden M. A. Antigenemia in primary coccidioidomycosis. Am J Trop Med Hyg. 1984 Jul;33(4):645–649. doi: 10.4269/ajtmh.1984.33.645. [DOI] [PubMed] [Google Scholar]
  4. Garcia N. M., Del Negro G. M., Martins H. P., Lacaz C. da S. Detection of paracoccidioidomycosis circulating antigen by the immunoelectroosmophoresis-immunodiffusion technique. Preliminary report. Rev Inst Med Trop Sao Paulo. 1987 Sep-Oct;29(5):327–328. doi: 10.1590/s0036-46651987000500011. [DOI] [PubMed] [Google Scholar]
  5. Goodman J. S., Kaufman L., Koenig M. G. Diagnosis of cryptococcal meningitis. Value of immunologic detection of cryptococcal antigen. N Engl J Med. 1971 Aug 19;285(8):434–436. doi: 10.1056/NEJM197108192850804. [DOI] [PubMed] [Google Scholar]
  6. Jimenez-Finkel B. E., Murphy J. W. Induction of antigen-specific T suppressor cells by soluble Paracoccidioides brasiliensis antigen. Infect Immun. 1988 Apr;56(4):734–743. doi: 10.1128/iai.56.4.734-743.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kanetsuna F., Carbonell L. M., Moreno R. E., Rodriguez J. Cell wall composition of the yeast and mycelial forms of Paracoccidioides brasiliensis. J Bacteriol. 1969 Mar;97(3):1036–1041. doi: 10.1128/jb.97.3.1036-1041.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Matthews R. C., Burnie J. P., Tabaqchali S. Isolation of immunodominant antigens from sera of patients with systemic candidiasis and characterization of serological response to Candida albicans. J Clin Microbiol. 1987 Feb;25(2):230–237. doi: 10.1128/jcm.25.2.230-237.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Matthews R., Burnie J. Diagnosis of systemic candidiasis by an enzyme-linked dot immunobinding assay for a circulating immunodominant 47-kilodalton antigen. J Clin Microbiol. 1988 Mar;26(3):459–463. doi: 10.1128/jcm.26.3.459-463.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Meckstroth K. L., Reiss E., Keller J. W., Kaufman L. Detection of antibodies and antigenemia in leukemic patients with candidiasis by enzyme-linked immunosorbent assay. J Infect Dis. 1981 Jul;144(1):24–32. doi: 10.1093/infdis/144.1.24. [DOI] [PubMed] [Google Scholar]
  12. Mendes-Giannini M. J., Camargo M. E., Lacaz C. S., Ferreira A. W. Immunoenzymatic absorption test for serodiagnosis of paracoccidioidomycosis. J Clin Microbiol. 1984 Jul;20(1):103–108. doi: 10.1128/jcm.20.1.103-108.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Puccia R., Schenkman S., Gorin P. A., Travassos L. R. Exocellular components of Paracoccidioides brasiliensis: identification of a specific antigen. Infect Immun. 1986 Jul;53(1):199–206. doi: 10.1128/iai.53.1.199-206.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reiss E., Lehmann P. F. Galactomannan antigenemia in invasive aspergillosis. Infect Immun. 1979 Jul;25(1):357–365. doi: 10.1128/iai.25.1.357-365.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rodrigues M. C., Cassaguerra C. M., Lacaz C. da S. Antigenemia in paracoccidioidomycosis. Probable demonstration of circulating antigen by counterimmunoelectrophoresis test. Preliminary report. Rev Inst Med Trop Sao Paulo. 1984 Sep-Oct;26(5):285–287. doi: 10.1590/s0036-46651984000500011. [DOI] [PubMed] [Google Scholar]
  16. San-Blas G. Paracoccidioides brasiliensis: cell wall glucans, pathogenicity, and dimorphism. Curr Top Med Mycol. 1985;1:235–257. doi: 10.1007/978-1-4613-9547-8_9. [DOI] [PubMed] [Google Scholar]
  17. Snow R. M., Dismukes W. E. Cryptococcal meningitis: diagnostic value of cryptococcal antigen in cerebrospinal fluid. Arch Intern Med. 1975 Sep;135(9):1155–1157. doi: 10.1001/archinte.135.9.1155. [DOI] [PubMed] [Google Scholar]
  18. Towbin H., Gordon J. Immunoblotting and dot immunobinding--current status and outlook. J Immunol Methods. 1984 Sep 4;72(2):313–340. doi: 10.1016/0022-1759(84)90001-2. [DOI] [PubMed] [Google Scholar]
  19. Weiner M. H. Antigenemia detected in human coccidioidomycosis. J Clin Microbiol. 1983 Jul;18(1):136–142. doi: 10.1128/jcm.18.1.136-142.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weiner M. H., Coats-Stephen M. Immunodiagnosis of systemic aspergillosis. I. Antigenemia detected by radioimmunoassay in experimental infection. J Lab Clin Med. 1979 Jan;93(1):111–119. [PubMed] [Google Scholar]
  21. Weiner M. H., Talbot G. H., Gerson S. L., Filice G., Cassileth P. A. Antigen detection in the diagnosis of invasive aspergillosis. Utility in controlled, blinded trials. Ann Intern Med. 1983 Dec;99(6):777–782. doi: 10.7326/0003-4819-99-6-777. [DOI] [PubMed] [Google Scholar]
  22. Wheat L. J., Kohler R. B., Tewari R. P. Diagnosis of disseminated histoplasmosis by detection of Histoplasma capsulatum antigen in serum and urine specimens. N Engl J Med. 1986 Jan 9;314(2):83–88. doi: 10.1056/NEJM198601093140205. [DOI] [PubMed] [Google Scholar]
  23. Yarzabal L. A., Andrieu S., Bout D., Naquira F. Isolation of a specific antigen with alkaline phosphatase activity from soluble extracts of Paracoccidioides brasiliensis. Sabouraudia. 1976 Nov;14(3):275–280. doi: 10.1080/00362177685190411. [DOI] [PubMed] [Google Scholar]
  24. Yarzabal L. A., Bout D., Naquira F., Fruit J., Andrieu S. Identification and purification of the specific antigen of Paracoccidioides brasiliensis responsible for immunoelectrophoretic band E. Sabouraudia. 1977 Mar;15(1):79–85. [PubMed] [Google Scholar]
  25. de Repentigny L., Reiss E. Current trends in immunodiagnosis of candidiasis and aspergillosis. Rev Infect Dis. 1984 May-Jun;6(3):301–312. doi: 10.1093/clinids/6.3.301. [DOI] [PubMed] [Google Scholar]

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