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. 1994 Apr;32(4):1023–1026. doi: 10.1128/jcm.32.4.1023-1026.1994

Investigation of hospital-acquired infections due to Alcaligenes denitrificans subsp. xylosoxydans by DNA restriction fragment length polymorphism.

M Cheron 1, E Abachin 1, E Guerot 1, M el-Bez 1, M Simonet 1
PMCID: PMC267173  PMID: 7913093

Abstract

We demonstrate that DNA restriction fragment length polymorphism determined by pulsed-field gel electrophoresis is very useful in the investigation of the epidemiology of hospital-acquired infections caused by Alcaligenes denitrificans subsp. xylosoxydans. This approach showed that hospital-acquired infections caused by this opportunistic pathogen over a 6-month period in 10 patients hospitalized in an intensive care unit and a surgical unit were not a true outbreak. In addition, this molecular typing method established that the respiratory therapy equipment was the source of the contamination of two patients.

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Selected References

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