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. 1989 Jan;27(1):13–20. doi: 10.1128/jcm.27.1.13-20.1989

Antigenically variable Borrelia burgdorferi isolated from cottontail rabbits and Ixodes dentatus in rural and urban areas.

J F Anderson 1, L A Magnarelli 1, R B LeFebvre 1, T G Andreadis 1, J B McAninch 1, G C Perng 1, R C Johnson 1
PMCID: PMC267224  PMID: 2913024

Abstract

Spirochetes were isolated from 71 subadult Ixodes dentatus removed from cottontail rabbits captured in Millbrook, N.Y., and in New York, N.Y. Spirochetes were also cultured from kidney tissues of six rabbits. While all isolates reacted with monoclonal antibody H9724, which identifies the spirochetes as borreliae, more than half did not bind with antibody H5332 and even fewer reacted with H3TS, both of which were produced to outer surface protein A of Borrelia burgdorferi. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profiles of three isolates differed from one another and from all previously characterized B. burgdorferi strains from humans, ticks, and wildlife in North America. The 12 periplasmic flagella that originated subterminally from each pointed end of a rabbit Borellia isolate contrasted with the 11 or fewer flagella for B. burgdorferi reported previously from North America. Although DNA homology and restriction endonuclease analysis also revealed differences among a rabbit kidney isolate, an I. dentatus isolate, and B. burgdorferi B31, similarities were sufficient to lead us to conclude that the borreliae in rabbits and I. dentatus are B. burgdorferi. Enzyme-linked immunosorbent assay titers of sera from humans with diagnosed Lyme disease to rabbit tick B. burgdorferi were often similar to one another and to those recorded for a reference B. burgdorferi strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. F., Duray P. H., Magnarelli L. A. Prevalence of Borrelia burgdorferi in white-footed mice and Ixodes dammini at Fort McCoy, Wis. J Clin Microbiol. 1987 Aug;25(8):1495–1497. doi: 10.1128/jcm.25.8.1495-1497.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson J. F., Johnson R. C., Magnarelli L. A., Hyde F. W., Andreadis T. G. New infectious spirochete isolated from short-tailed shrews and white-footed mice. J Clin Microbiol. 1987 Aug;25(8):1490–1494. doi: 10.1128/jcm.25.8.1490-1494.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Anderson J. F., Johnson R. C., Magnarelli L. A., Hyde F. W. Identification of endemic foci of Lyme disease: isolation of Borrelia burgdorferi from feral rodents and ticks (Dermacentor variabilis). J Clin Microbiol. 1985 Jul;22(1):36–38. doi: 10.1128/jcm.22.1.36-38.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Anderson J. F., Johnson R. C., Magnarelli L. A., Hyde F. W. Involvement of birds in the epidemiology of the Lyme disease agent Borrelia burgdorferi. Infect Immun. 1986 Feb;51(2):394–396. doi: 10.1128/iai.51.2.394-396.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Anderson J. F., Johnson R. C., Magnarelli L. A., Hyde F. W., Myers J. E. Peromyscus leucopus and Microtus pennsylvanicus simultaneously infected with Borrelia burgdorferi and Babesia microti. J Clin Microbiol. 1986 Jan;23(1):135–137. doi: 10.1128/jcm.23.1.135-137.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Anderson J. F., Magnarelli L. A., Burgdorfer W., Barbour A. G. Spirochetes in Ixodes dammini and mammals from Connecticut. Am J Trop Med Hyg. 1983 Jul;32(4):818–824. doi: 10.4269/ajtmh.1983.32.818. [DOI] [PubMed] [Google Scholar]
  7. Barbour A. G., Hayes S. F. Biology of Borrelia species. Microbiol Rev. 1986 Dec;50(4):381–400. doi: 10.1128/mr.50.4.381-400.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Barbour A. G., Hayes S. F., Heiland R. A., Schrumpf M. E., Tessier S. L. A Borrelia-specific monoclonal antibody binds to a flagellar epitope. Infect Immun. 1986 May;52(2):549–554. doi: 10.1128/iai.52.2.549-554.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Barbour A. G., Heiland R. A., Howe T. R. Heterogeneity of major proteins in Lyme disease borreliae: a molecular analysis of North American and European isolates. J Infect Dis. 1985 Sep;152(3):478–484. doi: 10.1093/infdis/152.3.478. [DOI] [PubMed] [Google Scholar]
  10. Barbour A. G. Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med. 1984 Jul-Aug;57(4):521–525. [PMC free article] [PubMed] [Google Scholar]
  11. Barbour A. G., Tessier S. L., Hayes S. F. Variation in a major surface protein of Lyme disease spirochetes. Infect Immun. 1984 Jul;45(1):94–100. doi: 10.1128/iai.45.1.94-100.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Barbour A. G., Tessier S. L., Todd W. J. Lyme disease spirochetes and ixodid tick spirochetes share a common surface antigenic determinant defined by a monoclonal antibody. Infect Immun. 1983 Aug;41(2):795–804. doi: 10.1128/iai.41.2.795-804.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Benach J. L., Bosler E. M., Hanrahan J. P., Coleman J. L., Habicht G. S., Bast T. F., Cameron D. J., Ziegler J. L., Barbour A. G., Burgdorfer W. Spirochetes isolated from the blood of two patients with Lyme disease. N Engl J Med. 1983 Mar 31;308(13):740–742. doi: 10.1056/NEJM198303313081302. [DOI] [PubMed] [Google Scholar]
  14. Bissett M. L., Hill W. Characterization of Borrelia burgdorferi strains isolated from Ixodes pacificus ticks in California. J Clin Microbiol. 1987 Dec;25(12):2296–2301. doi: 10.1128/jcm.25.12.2296-2301.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Bosler E. M., Coleman J. L., Benach J. L., Massey D. A., Hanrahan J. P., Burgdorfer W., Barbour A. G. Natural Distribution of the Ixodes dammini spirochete. Science. 1983 Apr 15;220(4594):321–322. doi: 10.1126/science.6836274. [DOI] [PubMed] [Google Scholar]
  16. Burgdorfer W., Barbour A. G., Hayes S. F., Benach J. L., Grunwaldt E., Davis J. P. Lyme disease-a tick-borne spirochetosis? Science. 1982 Jun 18;216(4552):1317–1319. doi: 10.1126/science.7043737. [DOI] [PubMed] [Google Scholar]
  17. Burgdorfer W., Lane R. S., Barbour A. G., Gresbrink R. A., Anderson J. R. The western black-legged tick, Ixodes pacificus: a vector of Borrelia burgdorferi. Am J Trop Med Hyg. 1985 Sep;34(5):925–930. doi: 10.4269/ajtmh.1985.34.925. [DOI] [PubMed] [Google Scholar]
  18. Burgdorfer W. The enlarging spectrum of tick-borne spirochetoses: R. R. Parker Memorial Address. Rev Infect Dis. 1986 Nov-Dec;8(6):932–940. doi: 10.1093/clinids/8.6.932. [DOI] [PubMed] [Google Scholar]
  19. Burgess E. C. Natural exposure of Wisconsin dogs to the Lyme disease spirochete (Borrelia burgdorferi). Lab Anim Sci. 1986 Jun;36(3):288–290. [PubMed] [Google Scholar]
  20. Godsey M. S., Jr, Amundson T. E., Burgess E. C., Schell W., Davis J. P., Kaslow R., Edelman R. Lyme disease ecology in Wisconsin: distribution and host preferences of Ixodes dammini, and prevalence of antibody to Borrelia burgdorferi in small mammals. Am J Trop Med Hyg. 1987 Jul;37(1):180–187. doi: 10.4269/ajtmh.1987.37.180. [DOI] [PubMed] [Google Scholar]
  21. Hovind-Hougen K., Asbrink E., Stiernstedt G., Steere A. C., Hovmark A. Ultrastructural differences among spirochetes isolated from patients with Lyme disease and related disorders, and from Ixodes ricinus. Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):103–111. doi: 10.1016/s0176-6724(86)80109-2. [DOI] [PubMed] [Google Scholar]
  22. Johnson R. C., Marek N., Kodner C. Infection of Syrian hamsters with Lyme disease spirochetes. J Clin Microbiol. 1984 Dec;20(6):1099–1101. doi: 10.1128/jcm.20.6.1099-1101.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Johnson S. E., Klein G. C., Schmid G. P., Bowen G. S., Feeley J. C., Schulze T. Lyme disease: a selective medium for isolation of the suspected etiological agent, a spirochete. J Clin Microbiol. 1984 Jan;19(1):81–82. doi: 10.1128/jcm.19.1.81-82.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Keirans J. E., Clifford C. M. The genus Ixodes in the United States: a scanning electron microscope study and key to the adults. J Med Entomol Suppl. 1978 Jul 20;2:1–149. doi: 10.1093/jmedent/15.suppl2.1. [DOI] [PubMed] [Google Scholar]
  25. Krampitz H. E. In vivo isolation and maintenance of some wild strains of European hard tick spirochetes in mammalian and arthropod hosts. A parasitologist's view. Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):21–28. doi: 10.1016/s0176-6724(86)80097-9. [DOI] [PubMed] [Google Scholar]
  26. Lane R. S., Burgdorfer W. Spirochetes in mammals and ticks (Acari: Ixodidae) from a focus of Lyme borreliosis in California. J Wildl Dis. 1988 Jan;24(1):1–9. doi: 10.7589/0090-3558-24.1.1. [DOI] [PubMed] [Google Scholar]
  27. Le Febvre R. B., Foley J. W., Thiermann A. B. Rapid and simplified protocol for isolation and characterization of leptospiral chromosomal DNA for taxonomy and diagnosis. J Clin Microbiol. 1985 Oct;22(4):606–608. doi: 10.1128/jcm.22.4.606-608.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Levine J. F., Wilson M. L., Spielman A. Mice as reservoirs of the Lyme disease spirochete. Am J Trop Med Hyg. 1985 Mar;34(2):355–360. doi: 10.4269/ajtmh.1985.34.355. [DOI] [PubMed] [Google Scholar]
  29. Loken K. I., Wu C. C., Johnson R. C., Bey R. F. Isolation of the Lyme disease spirochete from mammals in Minnesota. Proc Soc Exp Biol Med. 1985 Jul;179(3):300–302. doi: 10.3181/00379727-179-42100. [DOI] [PubMed] [Google Scholar]
  30. Magnarelli L. A., Anderson J. F., Apperson C. S., Fish D., Johnson R. C., Chappell W. A. Spirochetes in ticks and antibodies to Borrelia burgdorferi in white-tailed deer from Connecticut, New York State, and North Carolina. J Wildl Dis. 1986 Apr;22(2):178–188. doi: 10.7589/0090-3558-22.2.178. [DOI] [PubMed] [Google Scholar]
  31. Magnarelli L. A., Meegan J. M., Anderson J. F., Chappell W. A. Comparison of an indirect fluorescent-antibody test with an enzyme-linked immunosorbent assay for serological studies of Lyme disease. J Clin Microbiol. 1984 Aug;20(2):181–184. doi: 10.1128/jcm.20.2.181-184.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Rawlings J. A. Lyme disease in Texas. Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Feb;263(3):483–487. doi: 10.1016/s0176-6724(87)80115-3. [DOI] [PubMed] [Google Scholar]
  33. Schmid G. P., Steigerwalt A. G., Johnson S. E., Barbour A. G., Steere A. C., Robinson I. M., Brenner D. J. DNA characterization of the spirochete that causes Lyme disease. J Clin Microbiol. 1984 Aug;20(2):155–158. doi: 10.1128/jcm.20.2.155-158.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Schwan T. G., Burgdorfer W. Antigenic changes of Borrelia burgdorferi as a result of in vitro cultivation. J Infect Dis. 1987 Nov;156(5):852–853. doi: 10.1093/infdis/156.5.852-a. [DOI] [PubMed] [Google Scholar]
  35. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  36. Spielman A., Clifford C. M., Piesman J., Corwin M. D. Human babesiosis on Nantucket Island, USA: description of the vector, Ixodes (Ixodes) dammini, n. sp. (Acarina: Ixodidae). J Med Entomol. 1979 Mar 23;15(3):218–234. doi: 10.1093/jmedent/15.3.218. [DOI] [PubMed] [Google Scholar]
  37. Stanek G., Wewalka G., Groh V., Neumann R., Kristoferitsch W. Differences between Lyme disease and European arthropod-borne Borrelia infections. Lancet. 1985 Feb 16;1(8425):401–401. doi: 10.1016/s0140-6736(85)91424-2. [DOI] [PubMed] [Google Scholar]
  38. Steere A. C., Grodzicki R. L., Kornblatt A. N., Craft J. E., Barbour A. G., Burgdorfer W., Schmid G. P., Johnson E., Malawista S. E. The spirochetal etiology of Lyme disease. N Engl J Med. 1983 Mar 31;308(13):733–740. doi: 10.1056/NEJM198303313081301. [DOI] [PubMed] [Google Scholar]
  39. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Wilske B., Preac-Mursic V., Schierz G. Antigenic heterogeneity of European Borrelia burgdorferi strains isolated from patients and ticks. Lancet. 1985 May 11;1(8437):1099–1099. doi: 10.1016/s0140-6736(85)92396-7. [DOI] [PubMed] [Google Scholar]
  41. Wilske B., Preac-Mursic V., Schierz G., Busch K. V. Immunochemical and immunological analysis of European Borrelia burgdorferi strains. Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):92–102. doi: 10.1016/s0176-6724(86)80108-0. [DOI] [PubMed] [Google Scholar]

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