Abstract
We compared urine culturing performed by the calibrated loop method with a screening system (BACTEC). A total of 852 urine specimens were examined by both the conventional loop method and the BACTEC system. With the loop method, 193 (22.6%) urine samples were positive, whereas with the BACTEC system, 185 (21.7%) were positive (sensitivity, 96.01%). At a breakpoint of 10(4) CFU/ml, eight false-negatives were detected (sensitivity, 87.09%), and at a breakpoint of 10(5) CFU/ml, four false-negatives were observed (sensitivity, 97.6%). The specificity of the BACTEC system was 100%. We propose the BACTEC method as an effective alternative to the other growth-dependent screening tests for bacteriuria.
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Selected References
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- Alexander M. K., Khan M. S., Dow C. S. Rapid screening for bacteriuria using a particle counter, pulse-height analyser, and computer. J Clin Pathol. 1981 Feb;34(2):194–198. doi: 10.1136/jcp.34.2.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bixler-Forell E., Bertram M. A., Bruckner D. A. Clinical evaluation of three rapid methods for the detection of significant bacteriuria. J Clin Microbiol. 1985 Jul;22(1):62–67. doi: 10.1128/jcm.22.1.62-67.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cady P., Dufour S. W., Lawless P., Nunke B., Kraeger S. J. Impedimetric screening for bacteriuria. J Clin Microbiol. 1978 Mar;7(3):273–278. doi: 10.1128/jcm.7.3.273-278.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hale D. C., Wright D. N., McKie J. E., Isenberg H. D., Jenkins R. D., Matsen J. M. Rapid screening for bacteriuria by light scatter photometry (Autobac): a collaborative study. J Clin Microbiol. 1981 Jan;13(1):147–150. doi: 10.1128/jcm.13.1.147-150.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lewis J. F., Alexander J. Microscopy of stained urine smears to determine the need for quantitative culture. J Clin Microbiol. 1976 Oct;4(4):372–374. doi: 10.1128/jcm.4.4.372-374.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nicholson D. P., Koepke J. A. The Automicrobic System for urines. J Clin Microbiol. 1979 Dec;10(6):823–833. doi: 10.1128/jcm.10.6.823-833.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scherstén B., Dahlqvist A., Fritz H., Köhler L., Westlund L. Screening for bacteriuria with a tet paper for glucose. JAMA. 1968 Apr 15;204(3):205–208. doi: 10.1001/jama.1968.03140160015004. [DOI] [PubMed] [Google Scholar]
- Shaw S. T., Jr, Hopp M., St Geme J. W., Jr Quantitative urine culture with calibrated bacteriologic loop: office laboratory technique. Minn Med. 1966 May;49(5):749–752. [PubMed] [Google Scholar]
- Smalley D. L., Dittmann A. N. Use of leukocyte esterase-nitrate activity as predictive assays of significant bacteriuria. J Clin Microbiol. 1983 Nov;18(5):1256–1257. doi: 10.1128/jcm.18.5.1256-1257.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wallis C., Melnick J. L., Longoria C. J. Colorimetric method for rapid determination of bacteriuria. J Clin Microbiol. 1981 Sep;14(3):342–346. doi: 10.1128/jcm.14.3.342-346.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wu T. C., Williams E. C., Koo S. Y., MacLowry J. D. Evaluation of three bacteriuria screening methods in a clinical research hospital. J Clin Microbiol. 1985 May;21(5):796–799. doi: 10.1128/jcm.21.5.796-799.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]