Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1989 Mar;27(3):536–539. doi: 10.1128/jcm.27.3.536-539.1989

Taxonomic investigation of Legionella pneumophila using monoclonal antibodies.

R J Brindle 1, T N Bryant 1, P W Draper 1
PMCID: PMC267353  PMID: 2654183

Abstract

A panel of 19 monoclonal antibodies was used to produce patterns of immunofluorescent staining of 468 isolates of Legionella pneumophila. Twelve monoclonal antibodies were selected that divided L. pneumophila into 17 phenons which, in the majority of cases, conform to serogroup divisions. These phenons are more easily defined than the present serogroups, and isolates can be placed in them with little ambiguity. The standardized set of monoclonal antibodies was also used to define the subgroups of serogroup 1.

Full text

PDF
538

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benson R. F., Thacker W. L., Wilkinson H. W., Fallon R. J., Brenner D. J. Legionella pneumophila serogroup 14 isolated from patients with fatal pneumonia. J Clin Microbiol. 1988 Feb;26(2):382–382. doi: 10.1128/jcm.26.2.382-.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bibb W. F., Arnow P. M., Dellinger D. L., Perryman S. R. Isolation and characterization of a seventh serogroup of Legionella pneumophila. J Clin Microbiol. 1983 Feb;17(2):346–348. doi: 10.1128/jcm.17.2.346-348.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bissett M. L., Lee J. O., Lindquist D. S. New serogroup of Legionella pneumophila, serogroup 8. J Clin Microbiol. 1983 May;17(5):887–891. doi: 10.1128/jcm.17.5.887-891.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brindle R. J., Stannett P. J., Tobin J. O. Legionella pneumophila: monoclonal antibody typing of clinical and environmental isolates. Epidemiol Infect. 1987 Oct;99(2):235–239. doi: 10.1017/s0950268800067698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bryant T. N. Programs for evaluating and characterising bacterial taxonomic data. Comput Appl Biosci. 1987 Mar;3(1):45–48. doi: 10.1093/bioinformatics/3.1.45. [DOI] [PubMed] [Google Scholar]
  6. Edelstein P. H., Bibb W. F., Gorman G. W., Thacker W. L., Brenner D. J., Wilkinson H. W., Moss C. W., Buddington R. S., Dunn C. J., Roos P. J. Legionella pneumophila serogroup 9: a cause of human pneumonia. Ann Intern Med. 1984 Aug;101(2):196–198. doi: 10.7326/0003-4819-101-2-196. [DOI] [PubMed] [Google Scholar]
  7. Garrity G. M., Elder E. M., Davis B., Vickers R. M., Brown A. Serological and genotypic diversity among serogroup 5- reacting environmental Legionella isolates. J Clin Microbiol. 1982 Apr;15(4):646–653. doi: 10.1128/jcm.15.4.646-653.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Joly J. R., McKinney R. M., Tobin J. O., Bibb W. F., Watkins I. D., Ramsay D. Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J Clin Microbiol. 1986 Apr;23(4):768–771. doi: 10.1128/jcm.23.4.768-771.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McKinney R. M., Thacker L., Harris P. P., Lewallen K. R., Hebert G. A., Edelstein P. H., Thomason B. M. Four serogroups of Legionnaires' disease bacteria defined by direct immunofluorescence. Ann Intern Med. 1979 Apr;90(4):621–624. doi: 10.7326/0003-4819-90-4-621. [DOI] [PubMed] [Google Scholar]
  10. McKinney R. M., Wilkinson H. W., Sommers H. M., Fikes B. J., Sasseville K. R., Yungbluth M. M., Wolf J. S. Legionella pneumophila serogroup six: isolation from cases of legionellosis, identification by immunofluorescence staining, and immunological response to infection. J Clin Microbiol. 1980 Sep;12(3):395–401. doi: 10.1128/jcm.12.3.395-401.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Selander R. K., McKinney R. M., Whittam T. S., Bibb W. F., Brenner D. J., Nolte F. S., Pattison P. E. Genetic structure of populations of Legionella pneumophila. J Bacteriol. 1985 Sep;163(3):1021–1037. doi: 10.1128/jb.163.3.1021-1037.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Watkins I. D., Tobin J. O., Dennis P. J., Brown W., Newnham R., Kurtz J. B. Legionella pneumophila serogroup 1 subgrouping by monoclonal antibodies--an epidemiological tool. J Hyg (Lond) 1985 Oct;95(2):211–216. doi: 10.1017/s0022172400062641. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES