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. 1989 Apr;27(4):735–737. doi: 10.1128/jcm.27.4.735-737.1989

Characterization of CDC group DF-3 by cellular fatty acid analysis.

P L Wallace 1, D G Hollis 1, R E Weaver 1, C W Moss 1
PMCID: PMC267407  PMID: 2542365

Abstract

Fourteen strains of Centers for Disease Control group DF-3 bacteria were examined for cellular fatty acid composition to evaluate their chemical relatedness to known bacterial species and groups. The fatty acids were liberated from whole cells by base hydrolysis, methylated, and analyzed by capillary gas-liquid chromatography. All group DF-3 strains possessed a distinct fatty acid profile which was characterized by large amounts (24%) of 12-methyltetradecanoate (a-C15:0), moderate amounts of saturated iso-branched-chain acids (i-C14:0 and i-C15:0), and small to moderate amounts of both branched- and straight-chain hydroxy acids (3-OH C15:0, i-3-OH C16:0, 3-OH C16:0, and i-3-OH C17:0). This fatty acid profile was unique as compared with the profiles of other bacteria we have previously tested but was most similar to the profiles of Capnocytophaga species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boon J. J., van de Graaf B., Schuyle P. J., de Lange F., de Leeuw J. W. The mass spectrometry of iso and anteiso monoenoic fatty acids. Lipids. 1977 Sep;12(9):717–721. doi: 10.1007/BF02570901. [DOI] [PubMed] [Google Scholar]
  2. Brenner D. J., Hollis D. G., Fanning G. R., Weaver R. E. Capnocytophaga canimorsus sp. nov. (formerly CDC group DF-2), a cause of septicemia following dog bite, and C. cynodegmi sp. nov., a cause of localized wound infection following dog bite. J Clin Microbiol. 1989 Feb;27(2):231–235. doi: 10.1128/jcm.27.2.231-235.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dees S. B., Karr D. E., Hollis D., Moss C. W. Cellular fatty acids of Capnocytophaga species. J Clin Microbiol. 1982 Nov;16(5):779–783. doi: 10.1128/jcm.16.5.779-783.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dees S. B., Moss C. W., Hollis D. G., Weaver R. E. Chemical characterization of Flavobacterium odoratum, Flavobacterium breve, and Flavobacterium-like groups IIe, IIh, and IIf. J Clin Microbiol. 1986 Feb;23(2):267–273. doi: 10.1128/jcm.23.2.267-273.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dees S. B., Powell J., Moss C. W., Hollis D. G., Weaver R. E. Cellular fatty acid composition of organisms frequently associated with human infections resulting from dog bites: Pasteurella multocida and groups of EF-4, IIj, M-5, and DF-2. J Clin Microbiol. 1981 Dec;14(6):612–616. doi: 10.1128/jcm.14.6.612-616.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Moss C. W., Dees S. B. Cellular fatty acids of Flavobacterium meningosepticum and Flavobacterium species group IIb. J Clin Microbiol. 1978 Dec;8(6):772–774. doi: 10.1128/jcm.8.6.772-774.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moss C. W., Dees S. B. Identification of microorganisms by gas chromatographic-mass spectrometric analysis of cellular fatty acids. J Chromatogr. 1975 Oct 29;112:594–604. doi: 10.1016/s0021-9673(00)99988-6. [DOI] [PubMed] [Google Scholar]
  8. Moss C. W. Gas-liquid chromatography as an analytical tool in microbiology. J Chromatogr. 1981 Jan 9;203:337–347. doi: 10.1016/s0021-9673(00)80305-2. [DOI] [PubMed] [Google Scholar]
  9. Moss C. W., Kai A., Lambert M. A., Patton C. Isoprenoid quinone content and cellular fatty acid composition of Campylobacter species. J Clin Microbiol. 1984 Jun;19(6):772–776. doi: 10.1128/jcm.19.6.772-776.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moss C. W., Wallace P. L., Hollis D. G., Weaver R. E. Cultural and chemical characterization of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis. J Clin Microbiol. 1988 Mar;26(3):484–492. doi: 10.1128/jcm.26.3.484-492.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wallace P. L., Hollis D. G., Weaver R. E., Moss C. W. Cellular fatty acid composition of Kingella species, Cardiobacterium hominis, and Eikenella corrodens. J Clin Microbiol. 1988 Aug;26(8):1592–1594. doi: 10.1128/jcm.26.8.1592-1594.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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