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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1989 Jul;27(7):1522–1526. doi: 10.1128/jcm.27.7.1522-1526.1989

Four-year study of rotavirus electropherotypes from cases of infantile diarrhea in Rome.

F M Ruggeri 1, M L Marziano 1, A Tinari 1, E Salvatori 1, G Donelli 1
PMCID: PMC267608  PMID: 2549088

Abstract

Rotavirus infections were detected in 210 of 675 children with acute diarrhea admitted to a major pediatric hospital in Rome from January 1982 through December 1985. Most of the patients with rotavirus infections were admitted during the winter season in both 1982 and 1985, whereas during the two intermediate years, cases occurred in all months. Among 84 rotavirus samples examined, 14 different electropherotypes were recognized, 2 of which largely predominated over the others. The two electropherotypes were particularly frequent in the 2 epidemic years, altogether accounting for 70.2% of the samples typed, and circulated in distinct periods. None of the viruses showed a short pattern of electrophoretic migration of the genome, indicating a minor involvement of subgroup I rotaviruses in hospitalization-requiring diarrheas occurring in the area surveyed.

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Selected References

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  1. Albert M. J., Bishop R. F., Shann F. A. Epidemiology of rotavirus diarrhea in the Highlands of Papua, New Guinea, in 1979, as revealed by electrophoresis of genome RNA. J Clin Microbiol. 1983 Jan;17(1):162–164. doi: 10.1128/jcm.17.1.162-164.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beards G. M., Flewett T. H. Serological characterisation of human rotaviruses propagated in cell cultures. Brief report. Arch Virol. 1984;80(2-3):231–237. doi: 10.1007/BF01310663. [DOI] [PubMed] [Google Scholar]
  3. Beards G. M. Polymorphism of genomic RNAs within rotavirus serotypes and subgroups. Arch Virol. 1982;74(1):65–70. doi: 10.1007/BF01320783. [DOI] [PubMed] [Google Scholar]
  4. Brandt C. D., Kim H. W., Rodriguez W. J., Arrobio J. O., Jeffries B. C., Stallings E. P., Lewis C., Miles A. J., Chanock R. M., Kapikian A. Z. Pediatric viral gastroenteritis during eight years of study. J Clin Microbiol. 1983 Jul;18(1):71–78. doi: 10.1128/jcm.18.1.71-78.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bridger J. C., Pocock D. H. Variation in virulence of bovine rotaviruses. J Hyg (Lond) 1986 Apr;96(2):257–264. doi: 10.1017/s0022172400066031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chiba S., Yokoyama T., Nakata S., Morita Y., Urasawa T., Taniguchi K., Urasawa S., Nakao T. Protective effect of naturally acquired homotypic and heterotypic rotavirus antibodies. Lancet. 1986 Aug 23;2(8504):417–421. doi: 10.1016/s0140-6736(86)92133-1. [DOI] [PubMed] [Google Scholar]
  7. Chiba Y., Miyazaki C., Makino Y., Mutanda L. N., Kibue A., Lichenga E. O., Tukei P. M. Rotavirus infection of young children in two districts of Kenya from 1982 to 1983 as analyzed by electrophoresis of genomic RNA. J Clin Microbiol. 1984 May;19(5):579–582. doi: 10.1128/jcm.19.5.579-582.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Clark H. F., Dolan K. T., Horton-Slight P., Palmer J., Plotkin S. A. Diverse serologic response to rotavirus infection of infants in a single epidemic. Pediatr Infect Dis. 1985 Nov-Dec;4(6):626–631. doi: 10.1097/00006454-198511000-00006. [DOI] [PubMed] [Google Scholar]
  9. Clark H. F., Hoshino Y., Bell L. M., Groff J., Hess G., Bachman P., Offit P. A. Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol. 1987 Sep;25(9):1757–1762. doi: 10.1128/jcm.25.9.1757-1762.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dimitrov D. H., Graham D. Y., Lopez J., Muchinik G., Velasco G., Stenback W. A., Estes M. K. RNA electropherotypes of human rotaviruses from North and South America. Bull World Health Organ. 1984;62(2):321–329. [PMC free article] [PubMed] [Google Scholar]
  11. Donelli G., Ruggeri F. M., Tinari A., Marziano M. L., Menichella D., Caione D., Concato C., Rocchi G., Vella S. A three-year diagnostic and epidemiological study on viral infantile diarrhoea in Rome. Epidemiol Infect. 1988 Apr;100(2):311–320. doi: 10.1017/s0950268800067443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Espejo R. T., Muñz O., Serafin F., Romero P. Shift in the prevalent human rotavirus detected by ribonucleic acid segment differences. Infect Immun. 1980 Feb;27(2):351–354. doi: 10.1128/iai.27.2.351-354.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Flores J., Midthun K., Hoshino Y., Green K., Gorziglia M., Kapikian A. Z., Chanock R. M. Conservation of the fourth gene among rotaviruses recovered from asymptomatic newborn infants and its possible role in attenuation. J Virol. 1986 Dec;60(3):972–979. doi: 10.1128/jvi.60.3.972-979.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Follett E. A., Sanders R. C., Beards G. M., Hundley F., Desselberger U. Molecular epidemiology of human rotaviruses. Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83. J Hyg (Lond) 1984 Apr;92(2):209–222. doi: 10.1017/s0022172400064238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gerna G., Passarani N., Sarasini A., Battaglia M. Characterization of serotypes of human rotavirus strains by solid-phase immune electron microscopy. J Infect Dis. 1985 Dec;152(6):1143–1151. doi: 10.1093/infdis/152.6.1143. [DOI] [PubMed] [Google Scholar]
  16. Gorziglia M., Hoshino Y., Nishikawa K., Maloy W. L., Jones R. W., Kapikian A. Z., Chanock R. M. Comparative sequence analysis of the genomic segment 6 of four rotaviruses each with a different subgroup specificity. J Gen Virol. 1988 Jul;69(Pt 7):1659–1669. doi: 10.1099/0022-1317-69-7-1659. [DOI] [PubMed] [Google Scholar]
  17. Gómez J. A., Biscotti E. L., Bercovich J. A., Grinstein S. Epidemiology of human rotaviruses in Argentina as determined by RNA genome electrophoresis. Intervirology. 1986;26(3):174–180. doi: 10.1159/000149698. [DOI] [PubMed] [Google Scholar]
  18. Herring A. J., Inglis N. F., Ojeh C. K., Snodgrass D. R., Menzies J. D. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol. 1982 Sep;16(3):473–477. doi: 10.1128/jcm.16.3.473-477.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Hoshino Y., Gorziglia M., Valdesuso J., Askaa J., Glass R. I., Kapikian A. Z. An equine rotavirus (FI-14 strain) which bears both subgroup I and subgroup II specificities on its VP6. Virology. 1987 Apr;157(2):488–496. doi: 10.1016/0042-6822(87)90291-1. [DOI] [PubMed] [Google Scholar]
  20. Hoshino Y., Sereno M. M., Midthun K., Flores J., Chanock R. M., Kapikian A. Z. Analysis by plaque reduction neutralization assay of intertypic rotaviruses suggests that gene reassortment occurs in vivo. J Clin Microbiol. 1987 Feb;25(2):290–294. doi: 10.1128/jcm.25.2.290-294.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Hoshino Y., Sereno M. M., Midthun K., Flores J., Kapikian A. Z., Chanock R. M. Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8701–8704. doi: 10.1073/pnas.82.24.8701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kalica A. R., Greenberg H. B., Espejo R. T., Flores J., Wyatt R. G., Kapikian A. Z., Chanock R. M. Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2. Infect Immun. 1981 Sep;33(3):958–961. doi: 10.1128/iai.33.3.958-961.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kalica A. R., Wyatt R. G., Kapikian A. Z. Detection of differences among human and animal rotaviruses, using analysis of viral RNA. J Am Vet Med Assoc. 1978 Sep 1;173(5 Pt 2):531–537. [PubMed] [Google Scholar]
  24. Kapikian A. Z., Cline W. L., Greenberg H. B., Wyatt R. G., Kalica A. R., Banks C. E., James H. D., Jr, Flores J., Chanock R. M. Antigenic characterization of human and animal rotaviruses by immune adherence hemagglutination assay (IAHA): evidence for distinctness of IAHA and neutralization antigens. Infect Immun. 1981 Aug;33(2):415–425. doi: 10.1128/iai.33.2.415-425.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Konno T., Suzuki H., Imai A., Kutsuzawa T., Ishida N., Katsushima N., Sakamoto M., Kitaoka S., Tsuboi R., Adachi M. A long-term survey of rotavirus infection in Japanese children with acute gastroenteritis. J Infect Dis. 1978 Nov;138(5):569–576. doi: 10.1093/infdis/138.5.569. [DOI] [PubMed] [Google Scholar]
  26. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  27. Matsuno S., Hasegawa A., Mukoyama A., Inouye S. A candidate for a new serotype of human rotavirus. J Virol. 1985 May;54(2):623–624. doi: 10.1128/jvi.54.2.623-624.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Matsuno S., Nagayoshi S. Quantitative estimation of infantile gastroenteritis virus antigens in stools by immune adherence hemagglutination test. J Clin Microbiol. 1978 Mar;7(3):310–311. doi: 10.1128/jcm.7.3.310-311.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Offit P. A., Blavat G. Identification of the two rotavirus genes determining neutralization specificities. J Virol. 1986 Jan;57(1):376–378. doi: 10.1128/jvi.57.1.376-378.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Rodger S. M., Bishop R. F., Birch C., McLean B., Holmes I. H. Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. J Clin Microbiol. 1981 Feb;13(2):272–278. doi: 10.1128/jcm.13.2.272-278.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Schnagl R. D., Rodger S. M., Holmes I. H. Variation in human rotavirus electropherotypes occurring between rotavirus gastroenteritis epidemics in central Australia. Infect Immun. 1981 Jul;33(1):17–21. doi: 10.1128/iai.33.1.17-21.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Shaw R. D., Vo P. T., Offit P. A., Coulson B. S., Greenberg H. B. Antigenic mapping of the surface proteins of rhesus rotavirus. Virology. 1986 Dec;155(2):434–451. doi: 10.1016/0042-6822(86)90205-9. [DOI] [PubMed] [Google Scholar]
  33. Steele A. D., Alexander J. J. Molecular epidemiology of rotavirus in black infants in South Africa. J Clin Microbiol. 1987 Dec;25(12):2384–2387. doi: 10.1128/jcm.25.12.2384-2387.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Tam J. S., Kum W. W., Lam B., Yeung C. Y., Ng M. H. Molecular epidemiology of human rotavirus infection in children in Hong Kong. J Clin Microbiol. 1986 Mar;23(3):660–664. doi: 10.1128/jcm.23.3.660-664.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Taniguchi K., Urasawa S., Urasawa T. Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. J Gen Virol. 1985 May;66(Pt 5):1045–1053. doi: 10.1099/0022-1317-66-5-1045. [DOI] [PubMed] [Google Scholar]
  36. Uhnoo I., Svensson L. Clinical and epidemiological features of acute infantile gastroenteritis associated with human rotavirus subgroups 1 and 2. J Clin Microbiol. 1986 Mar;23(3):551–555. doi: 10.1128/jcm.23.3.551-555.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Vesikari T., Isolauri E., Delem A., d'Hondt E., André F. E., Beards G. M., Flewett T. H. Clinical efficacy of the RIT 4237 live attenuated bovine rotavirus vaccine in infants vaccinated before a rotavirus epidemic. J Pediatr. 1985 Aug;107(2):189–194. doi: 10.1016/s0022-3476(85)80123-2. [DOI] [PubMed] [Google Scholar]
  38. Vesikari T., Sarkkinen H. K., Mäki M. Quantitative aspects of rotavirus excretion in childhood diarrhoea. Acta Paediatr Scand. 1981 Sep;70(5):717–721. doi: 10.1111/j.1651-2227.1981.tb05774.x. [DOI] [PubMed] [Google Scholar]
  39. Wyatt R. G., James H. D., Jr, Pittman A. L., Hoshino Y., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes. J Clin Microbiol. 1983 Aug;18(2):310–317. doi: 10.1128/jcm.18.2.310-317.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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