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. 1989 Aug;27(8):1854–1858. doi: 10.1128/jcm.27.8.1854-1858.1989

Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi.

K E Hechemy 1, H L Harris 1, J A Wethers 1, R W Stevens 1, B R Stock 1, A A Reilly 1, J L Benach 1
PMCID: PMC267683  PMID: 2671034

Abstract

Sodium deoxycholate-solubilized Borrelia burgdorferi antigen was prepared for use in a solid-phase fluoroimmunoassay (FIA-L) to detect antibodies in Lyme disease. Serum specimens were tested by FIA-L and by a microimmunofluorescence test. The FIA-L results are comparable to those of the standard microimmunofluorescence test. The overall agreement was 0.98. Moreover, the FIA-L procedure is simple and rapid; fluorescence is objectively determined and is proportional to antibody titer.

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Selected References

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