Abstract
We compared the growth of Bordetella pertussis strains (n = 32) on antibiotic-free and cephalexin (40 micrograms/ml)-containing charcoal agar supplemented with 10% defibrinated horse blood, defibrinated sheep blood, or anticoagulant-containing human blood. Plates were incubated either in air or in an atmosphere with 5 to 10% CO2. As assessed by mean colony numbers and rapidity of growth, normal air was preferable to CO2 enrichment for incubation. Growth on horse blood agar was more abundant and more rapid than on sheep blood agar, but the difference in general was not statistically significant. Human blood was clearly inferior to both horse and sheep blood.
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Selected References
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- Gilligan P. H., Fisher M. C. Importance of culture in laboratory diagnosis of Bordetella pertussis infections. J Clin Microbiol. 1984 Nov;20(5):891–893. doi: 10.1128/jcm.20.5.891-893.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoppe J. E. Methods for isolation of Bordetella pertussis from patients with whooping cough. Eur J Clin Microbiol Infect Dis. 1988 Oct;7(5):616–620. doi: 10.1007/BF01964238. [DOI] [PubMed] [Google Scholar]
- Marcon M. J., Hamoudi A. C., Cannon H. J., Hribar M. M. Comparison of throat and nasopharyngeal swab specimens for culture diagnosis of Bordetella pertussis infection. J Clin Microbiol. 1987 Jun;25(6):1109–1110. doi: 10.1128/jcm.25.6.1109-1110.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]