Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jun;28(6):1425–1427. doi: 10.1128/jcm.28.6.1425-1427.1990

Semiquantitative technique for estimating Pneumocystis carinii burden in the lung.

R P Baughman 1, S Strohofer 1, G Colangelo 1, P T Frame 1
PMCID: PMC267944  PMID: 2380366

Abstract

We developed a technique to estimate the amount of Pneumocystis carinii found in bronchoalveolar lavage fluid. P. carinii associated with 500 nucleated cells in the bronchoalveolar lavage fluid had little between-observer and within-observer variation. Varying the technique of the lavage did not change the amount of P. carinii recovered. This technique was used in patients treated for P. carinii pneumonia. Those patients who did not respond to treatment had more P. carinii in their bronchoalveolar lavage fluid than those who responded.

Full text

PDF
1425

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allegra C. J., Chabner B. A., Tuazon C. U., Ogata-Arakaki D., Baird B., Drake J. C., Simmons J. T., Lack E. E., Shelhamer J. H., Balis F. Trimetrexate for the treatment of Pneumocystis carinii pneumonia in patients with the acquired immunodeficiency syndrome. N Engl J Med. 1987 Oct 15;317(16):978–985. doi: 10.1056/NEJM198710153171602. [DOI] [PubMed] [Google Scholar]
  2. Barrio J. L., Harcup C., Baier H. J., Pitchenik A. E. Value of repeat fiberoptic bronchoscopies and significance of nondiagnostic bronchoscopic results in patients with the acquired immunodeficiency syndrome. Am Rev Respir Dis. 1987 Feb;135(2):422–425. doi: 10.1164/arrd.1987.135.2.422. [DOI] [PubMed] [Google Scholar]
  3. Baughman R., Strohofer S., Kim C. K. Variation of differential cell counts of bronchoalveolar lavage fluid. Arch Pathol Lab Med. 1986 Apr;110(4):341–343. [PubMed] [Google Scholar]
  4. Broaddus C., Dake M. D., Stulbarg M. S., Blumenfeld W., Hadley W. K., Golden J. A., Hopewell P. C. Bronchoalveolar lavage and transbronchial biopsy for the diagnosis of pulmonary infections in the acquired immunodeficiency syndrome. Ann Intern Med. 1985 Jun;102(6):747–752. doi: 10.7326/0003-4819-102-6-747. [DOI] [PubMed] [Google Scholar]
  5. Dohn M. N., Baughman R. P. Effect of changing instilled volume for bronchoalveolar lavage in patients with interstitial lung disease. Am Rev Respir Dis. 1985 Aug;132(2):390–392. doi: 10.1164/arrd.1985.132.2.390. [DOI] [PubMed] [Google Scholar]
  6. Golden J. A., Hollander H., Stulbarg M. S., Gamsu G. Bronchoalveolar lavage as the exclusive diagnostic modality for Pneumocystis carinii pneumonia. A prospective study among patients with acquired immunodeficiency syndrome. Chest. 1986 Jul;90(1):18–22. doi: 10.1378/chest.90.1.18. [DOI] [PubMed] [Google Scholar]
  7. Haverkos H. W. Assessment of therapy for pneumocystis carinii pneumonia. PCP Therapy Project Group. Am J Med. 1984 Mar;76(3):501–508. doi: 10.1016/0002-9343(84)90670-3. [DOI] [PubMed] [Google Scholar]
  8. Kim C. K., Foy J. M., Cushion M. T., Stanforth D., Linke M. J., Hendrix H. L., Walzer P. D. Comparison of histologic and quantitative techniques in evaluation of therapy for experimental Pneumocystis carinii pneumonia. Antimicrob Agents Chemother. 1987 Feb;31(2):197–201. doi: 10.1128/aac.31.2.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Murray J. F., Felton C. P., Garay S. M., Gottlieb M. S., Hopewell P. C., Stover D. E., Teirstein A. S. Pulmonary complications of the acquired immunodeficiency syndrome. Report of a National Heart, Lung, and Blood Institute workshop. N Engl J Med. 1984 Jun 21;310(25):1682–1688. doi: 10.1056/NEJM198406213102529. [DOI] [PubMed] [Google Scholar]
  10. Murray J. F., Garay S. M., Hopewell P. C., Mills J., Snider G. L., Stover D. E. NHLBI workshop summary. Pulmonary complications of the acquired immunodeficiency syndrome: an update. Report of the second National Heart, Lung and Blood Institute workshop. Am Rev Respir Dis. 1987 Feb;135(2):504–509. doi: 10.1164/arrd.1987.135.2.504. [DOI] [PubMed] [Google Scholar]
  11. Queener S. F., Bartlett M. S., Richardson J. D., Durkin M. M., Jay M. A., Smith J. W. Activity of clindamycin with primaquine against Pneumocystis carinii in vitro and in vivo. Antimicrob Agents Chemother. 1988 Jun;32(6):807–813. doi: 10.1128/aac.32.6.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sattler F. R., Cowan R., Nielsen D. M., Ruskin J. Trimethoprim-sulfamethoxazole compared with pentamidine for treatment of Pneumocystis carinii pneumonia in the acquired immunodeficiency syndrome. A prospective, noncrossover study. Ann Intern Med. 1988 Aug 15;109(4):280–287. doi: 10.7326/0003-4819-109-4-280. [DOI] [PubMed] [Google Scholar]
  13. Shelhamer J. H., Ognibene F. P., Macher A. M., Tuazon C., Steiss R., Longo D., Kovacs J. A., Parker M. M., Natanson C., Lane H. C. Persistence of Pneumocystis carinii in lung tissue of acquired immunodeficiency syndrome patients treated for pneumocystis pneumonia. Am Rev Respir Dis. 1984 Dec;130(6):1161–1165. doi: 10.1164/arrd.1984.130.6.1161. [DOI] [PubMed] [Google Scholar]
  14. Stover D. E., Zaman M. B., Hajdu S. I., Lange M., Gold J., Armstrong D. Bronchoalveolar lavage in the diagnosis of diffuse pulmonary infiltrates in the immunosuppressed host. Ann Intern Med. 1984 Jul;101(1):1–7. doi: 10.7326/0003-4819-101-1-1. [DOI] [PubMed] [Google Scholar]
  15. Thorpe J. E., Baughman R. P., Frame P. T., Wesseler T. A., Staneck J. L. Bronchoalveolar lavage for diagnosing acute bacterial pneumonia. J Infect Dis. 1987 May;155(5):855–861. doi: 10.1093/infdis/155.5.855. [DOI] [PubMed] [Google Scholar]
  16. Tollerud D. J., Wesseler T. A., Kim C. K., Baughman R. P. Use of a rapid differential stain for identifying Pneumocystis carinii in bronchoalveolar lavage fluid. Diagnostic efficacy in patients with AIDS. Chest. 1989 Mar;95(3):494–497. doi: 10.1378/chest.95.3.494. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES