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. 1990 Aug;28(8):1766–1769. doi: 10.1128/jcm.28.8.1766-1769.1990

Preparation of species-specific murine monoclonal antibodies against the yeast phase of Paracoccidioides brasiliensis.

J I Figueroa 1, A J Hamilton 1, M A Bartholomew 1, T Harada 1, L Fenelon 1, R J Hay 1
PMCID: PMC268044  PMID: 2394802

Abstract

A panel of four murine monoclonal antibodies showing species specificity for the yeast phase of the pathogenic dimorphic fungus Paracoccidioides brasiliensis was produced by using a modification of the standard monoclonal antibody technology. This involved the use of the immunosuppressive drug cyclophosphamide to suppress the immune response of test animals to fungi showing cross-reactivity, i.e., to Histoplasma capsulatum. One monoclonal antibody, P4, which had a high titer by enzyme-linked immunosorbent assay, was shown to recognize a linear antigenic epitope of P. brasiliensis at a molecular size of 70,000 to 75,000 daltons by Western blot (immunoblot) analysis. The potential use of these monoclonal antibodies, which are the first species-specific probes to P. brasiliensis that have been produced, in the field of serodiagnosis is discussed.

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Selected References

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  1. Biagioni L., Souza M. J., Chamma L. G., Mendes R. P., Marques S. A., Mota N. G., Franco M. Serology of paracoccidioidomycosis. II. Correlation between class-specific antibodies and clinical forms of the disease. Trans R Soc Trop Med Hyg. 1984;78(5):617–621. doi: 10.1016/0035-9203(84)90220-7. [DOI] [PubMed] [Google Scholar]
  2. Blumer S. O., Jalbert M., Kaufman L. Rapid and reliable method for production of a specific Paracoccidioides brasiliensis immunodiffusion test antigen. J Clin Microbiol. 1984 Mar;19(3):404–407. doi: 10.1128/jcm.19.3.404-407.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burkot T. R., Zavala F., Gwadz R. W., Collins F. H., Nussenzweig R. S., Roberts D. R. Identification of malaria-infected mosquitoes by a two-site enzyme-linked immunosorbent assay. Am J Trop Med Hyg. 1984 Mar;33(2):227–231. doi: 10.4269/ajtmh.1984.33.227. [DOI] [PubMed] [Google Scholar]
  4. Cano L. E., Brummer E., Stevens D. A., Restrepo A. An evaluation of the enzyme-linked immunoabsorbent assay (ELISA) for quantitation of antibodies to Paracoccidioides brasiliensis. J Med Vet Mycol. 1986 Dec;24(6):467–475. doi: 10.1080/02681218680000741. [DOI] [PubMed] [Google Scholar]
  5. Cano L. E., Restrepo A. Predictive value of serologic tests in the diagnosis and follow-up of patients with paracoccidioidomycosis. Rev Inst Med Trop Sao Paulo. 1987 Sep-Oct;29(5):276–283. doi: 10.1590/s0036-46651987000500003. [DOI] [PubMed] [Google Scholar]
  6. Castaneda E., Brummer E., Pappagianis D., Stevens D. A. Regulation of immune responses by T suppressor cells and by serum in chronic paracoccidioidomycosis. Cell Immunol. 1988 Nov;117(1):1–11. doi: 10.1016/0008-8749(88)90071-8. [DOI] [PubMed] [Google Scholar]
  7. De Camargo Z., Unterkircher C., Campoy S. P., Travassos L. R. Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests. J Clin Microbiol. 1988 Oct;26(10):2147–2151. doi: 10.1128/jcm.26.10.2147-2151.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fava Netto C. Imunologia da paracoccidioidomicose. Rev Inst Med Trop Sao Paulo. 1976 Jan-Feb;18(1):42–53. [PubMed] [Google Scholar]
  9. Franco M., Montenegro M. R., Mendes R. P., Marques S. A., Dillon N. L., Mota N. G. Paracoccidioidomycosis: a recently proposed classification of its clinical forms. Rev Soc Bras Med Trop. 1987 Apr-Jun;20(2):129–132. doi: 10.1590/s0037-86821987000200012. [DOI] [PubMed] [Google Scholar]
  10. Hamilton A. J., Bartholomew M. A., Fenelon L. E., Figueroa J., Hay R. J. A murine monoclonal antibody exhibiting high species specificity for Histoplasma capsulatum var. capsulatum. J Gen Microbiol. 1990 Feb;136(2):331–335. doi: 10.1099/00221287-136-2-331. [DOI] [PubMed] [Google Scholar]
  11. Hay R. J., Rose P., Jones T. R. Paracoccidioidin sensitization in Guyana--a preliminary skin test survey in hospitalized patients and laboratory workers. Trans R Soc Trop Med Hyg. 1987;81(1):46–48. doi: 10.1016/0035-9203(87)90277-x. [DOI] [PubMed] [Google Scholar]
  12. Kashino S. S., Calich V. L., Singer-Vermes L. M., Abrahamsohn P. A., Burger E. Growth curves, morphology and ultrastructure of ten Paracoccidioides brasiliensis isolates. Mycopathologia. 1987 Aug;99(2):119–128. doi: 10.1007/BF00436916. [DOI] [PubMed] [Google Scholar]
  13. Lal R. B., Paranjape R. S., Briles D. E., Nutman T. B., Ottesen E. A. Circulating parasite antigen(s) in lymphatic filariasis: use of monoclonal antibodies to phosphocholine for immunodiagnosis. J Immunol. 1987 May 15;138(10):3454–3460. [PubMed] [Google Scholar]
  14. Matthew W. D., Sandrock A. W., Jr Cyclophosphamide treatment used to manipulate the immune response for the production of monoclonal antibodies. J Immunol Methods. 1987 Jun 26;100(1-2):73–82. doi: 10.1016/0022-1759(87)90174-8. [DOI] [PubMed] [Google Scholar]
  15. Mistreta T., Souza M. J., Chamma L. G., Pinho S. Z., Franco M. Serology of paracoccidioidomycosis. I. Evaluation of the indirect immunofluorescent test. Mycopathologia. 1985 Jan;89(1):13–17. doi: 10.1007/BF00437127. [DOI] [PubMed] [Google Scholar]
  16. Moscardi-Bacchi M., Soares A., Mendes R., Marques S., Franco M. In situ localization of T lymphocyte subsets in human paracoccidioidomycosis. J Med Vet Mycol. 1989;27(3):149–158. [PubMed] [Google Scholar]
  17. Negroni R., De Flores C. I., Robles A. M. Estudio de las reacciones serologicas cruzadas entre antigenos de Paracoccidioides brasiliensis e Histoplasma capsulatum. Rev Asoc Argent Microbiol. 1976 May-Aug;8(2):68–73. [PubMed] [Google Scholar]
  18. Read S. M., Northcote D. H. Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal Biochem. 1981 Sep 1;116(1):53–64. doi: 10.1016/0003-2697(81)90321-3. [DOI] [PubMed] [Google Scholar]
  19. Restrepo A., Moncada L. H. Indirect fluorescent-antibody and quantitative agar-gel immunodiffusion tests for the serological diagnosis of paracoccidioidomycosis. Appl Microbiol. 1972 Jul;24(1):132–137. doi: 10.1128/am.24.1.132-137.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Restrepo A. The ecology of Paracoccidioides brasiliensis: a puzzle still unsolved. Sabouraudia. 1985 Oct;23(5):323–334. [PubMed] [Google Scholar]
  21. Salazar M. E., Restrepo A., Stevens D. A. Inhibition by estrogens of conidium-to-yeast conversion in the fungus Paracoccidioides brasiliensis. Infect Immun. 1988 Mar;56(3):711–713. doi: 10.1128/iai.56.3.711-713.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stambuk B. U., Puccia R., de Almeida M. L., Travassos L. R., Schenkman S. Secretion of the 43 kDa glycoprotein antigen by Paracoccidioides brasiliensis. J Med Vet Mycol. 1988;26(6):367–373. doi: 10.1080/02681218880000521. [DOI] [PubMed] [Google Scholar]
  23. Thomas W. A. Production of monoclonal antibodies selective for aggregation-competent chick neural retina cells. An immunosuppressive approach. J Immunol Methods. 1987 Mar 12;97(2):237–243. doi: 10.1016/0022-1759(87)90465-0. [DOI] [PubMed] [Google Scholar]
  24. Tsang V. C., Peralta J. M., Simons A. R. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol. 1983;92:377–391. doi: 10.1016/0076-6879(83)92032-3. [DOI] [PubMed] [Google Scholar]
  25. Weil G. J., Jain D. C., Santhanam S., Malhotra A., Kumar H., Sethumadhavan K. V., Liftis F., Ghosh T. K. A monoclonal antibody-based enzyme immunoassay for detecting parasite antigenemia in bancroftian filariasis. J Infect Dis. 1987 Aug;156(2):350–355. doi: 10.1093/infdis/156.2.350. [DOI] [PubMed] [Google Scholar]
  26. de Camargo Z. P., Guesdon J. L., Drouhet E., Improvisi L. Enzyme-linked immunosorbent assay (ELISA) in the paracoccidioidomycosis. Comparison with counterimmunoelectrophoresis and erythro-immunoassay. Mycopathologia. 1984 Oct 30;88(1):31–37. doi: 10.1007/BF00439292. [DOI] [PubMed] [Google Scholar]
  27. de Camargo Z. P., Guesdon J. L., Drouhet E., Improvisi L. Magnetic enzyme-linked immunosorbent assay (MELISA) for determination of specific IgG in paracoccidioidomycosis. Sabouraudia. 1984;22(4):291–299. doi: 10.1080/00362178485380491. [DOI] [PubMed] [Google Scholar]

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