Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Dec;28(12):2632–2636. doi: 10.1128/jcm.28.12.2632-2636.1990

Toxoplasma antigens recognized by human immunoglobulin A antibodies.

J Huskinson 1, P Thulliez 1, J S Remington 1
PMCID: PMC268247  PMID: 2279993

Abstract

The immunoglobulin A (IgA) antibody response to antigens of Toxoplasma gondii during the acute and chronic stages of T. gondii infection was studied by immunoblotting with a nonreduced antigen (NRA) preparation. Serum samples were from individuals with acute or chronic T. gondii infection, and sequential samples were from women who seroconverted during gestation or congenitally infected infants. IgA antibodies to a variety of antigens were present in sera from each of the groups, irrespective of the titer in an IgA enzyme-linked immunosorbent assay. The predominant NRA recognized by IgA antibodies in sera from each of the four groups had an approximate molecular weight of 30,000. Other prominent NRAs had approximate molecular weights of 35,000 and 5,000; however, the latter NRA was not present in sera of congenitally infected infants.

Full text

PDF
2633

Images in this article

2634Image
on p.2634
2634Image
on p.2634
2635Image
on p.2635
2635Image
on p.2635

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Decoster A., Darcy F., Caron A., Capron A. IgA antibodies against P30 as markers of congenital and acute toxoplasmosis. Lancet. 1988 Nov 12;2(8620):1104–1107. doi: 10.1016/s0140-6736(88)90523-5. [DOI] [PubMed] [Google Scholar]
  2. Derouin F., Sulcebe G., Ballet J. J. Sequential determination of IgG subclasses and IgA specific antibodies in primary and reactivating toxoplasmosis. Biomed Pharmacother. 1987;41(8):429–433. [PubMed] [Google Scholar]
  3. Desmonts G., Naot Y., Remington J. S. Immunoglobulin M-immunosorbent agglutination assay for diagnosis of infectious diseases: diagnosis of acute congenital and acquired Toxoplasma infections. J Clin Microbiol. 1981 Nov;14(5):486–491. doi: 10.1128/jcm.14.5.486-491.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Huskinson J., Stepick-Biek P. N., Araujo F. G., Thulliez P., Suzuki Y., Remington J. S. Toxoplasma antigens recognized by immunoglobulin G subclasses during acute and chronic infection. J Clin Microbiol. 1989 Sep;27(9):2031–2038. doi: 10.1128/jcm.27.9.2031-2038.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Le Fichoux Y., Marty P., Chan H. Les IgA sériques spécifiques dans le diagnostic de la toxoplasmose. Ann Pediatr (Paris) 1987 May;34(5):375–379. [PubMed] [Google Scholar]
  7. Partanen P., Turunen H. J., Paasivuo R. T., Leinikki P. O. Immunoblot analysis of Toxoplasma gondii antigens by human immunoglobulins G, M, and A antibodies at different stages of infection. J Clin Microbiol. 1984 Jul;20(1):133–135. doi: 10.1128/jcm.20.1.133-135.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pinon J. M., Thoannes H., Pouletty P. H., Poirriez J., Damiens J., Pelletier P. Detection of IgA specific for toxoplasmosis in serum and cerebrospinal fluid using a non-enzymatic IgA-capture assay. Diagn Immunol. 1986;4(5):223–227. [PubMed] [Google Scholar]
  9. Potasman I., Araujo F. G., Desmonts G., Remington J. S. Analysis of Toxoplasma gondii antigens recognized by human sera obtained before and after acute infection. J Infect Dis. 1986 Oct;154(4):650–657. doi: 10.1093/infdis/154.4.650. [DOI] [PubMed] [Google Scholar]
  10. Sabin A. B., Feldman H. A. Dyes as Microchemical Indicators of a New Immunity Phenomenon Affecting a Protozoon Parasite (Toxoplasma). Science. 1948 Dec 10;108(2815):660–663. doi: 10.1126/science.108.2815.660. [DOI] [PubMed] [Google Scholar]
  11. Siegel J. P., Remington J. S. Comparison of methods for quantitating antigen-specific immunoglobulin M antibody with a reverse enzyme-linked immunosorbent assay. J Clin Microbiol. 1983 Jul;18(1):63–70. doi: 10.1128/jcm.18.1.63-70.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stepick-Biek P., Thulliez P., Araujo F. G., Remington J. S. IgA antibodies for diagnosis of acute congenital and acquired toxoplasmosis. J Infect Dis. 1990 Jul;162(1):270–273. doi: 10.1093/infdis/162.1.270. [DOI] [PubMed] [Google Scholar]
  13. Wilson C. B., Tsai V., Remington J. S. Failure to trigger the oxidative metabolic burst by normal macrophages: possible mechanism for survival of intracellular pathogens. J Exp Med. 1980 Feb 1;151(2):328–346. doi: 10.1084/jem.151.2.328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. van Loon A. M., van der Logt J. T., Heessen F. W., van der Veen J. Enzyme-linked immunosorbent assay that uses labeled antigen for detection of immunoglobulin M and A antibodies in toxoplasmosis: comparison with indirect immunofluorescence and double-sandwich enzyme-linked immunosorbent assay. J Clin Microbiol. 1983 Jun;17(6):997–1004. doi: 10.1128/jcm.17.6.997-1004.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES