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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Dec;28(12):2842–2844. doi: 10.1128/jcm.28.12.2842-2844.1990

Oligonucleotide probe for detection of the enteropathogenic Escherichia coli (EPEC) adherence factor of localized adherent EPEC.

A E Jerse 1, W C Martin 1, J E Galen 1, J B Kaper 1
PMCID: PMC268291  PMID: 1980682

Abstract

The enteropathogenic Escherichia coli adherence factor (EAF) probe detects isolates of enteropathogenic E. coli that exhibit localized adherence to HEp-2 cells. A 21-base oligonucleotide probe was constructed on the basis of a sequence from within the 1-kb EAF probe and was shown to have greater sensitivity and specificity than the EAF fragment probe in detecting localized adherent E. coli.

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Selected References

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