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. 1985 Jul;22(1):26–31. doi: 10.1128/jcm.22.1.26-31.1985

Competitive, enzyme-linked immunosorbent assay for toxic shock syndrome toxin 1.

J Parsonnet, J T Mills, Z A Gillis, G B Pier
PMCID: PMC268314  PMID: 3926815

Abstract

We developed a competitive, enzyme-linked immunosorbent assay for the quantitation of toxic shock syndrome toxin 1 (TSST-1). Polyvalent immunoglobulin G from immunized rabbits was used as the capture antibody, and alkaline phosphatase conjugated to purified toxin served as the indicator enzyme. A standard curve was generated with each experiment, from which the concentration of toxin in culture supernatants was extrapolated. The assay was useful for determining toxin concentrations of 0.03 to 0.5 micrograms/ml, which is a substantial, practical improvement over immunodiffusion methods. Staphylococcal enterotoxins A through E were not significantly cross-reactive in the assay, and staphylococcal protein A did not interfere with quantitation of TSST-1. By testing a variety of staphylococcal strains, we found 100% concordance between toxin determinations made with our assay and those made by the investigators from whom the strains were obtained. The competitive, enzyme-linked immunosorbent assay is a highly reproducible, inexpensive means of determining TSST-1 concentrations and may have broad applicability in the field of toxic shock research.

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Selected References

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  1. Berdal B. P., Olsvik O., Omland T. A sandwich ELISA method for detection of Staphylococcus aureus enterotoxins. Acta Pathol Microbiol Scand B. 1981 Dec;89(6):411–415. [PubMed] [Google Scholar]
  2. Bergdoll M. S., Crass B. A., Reiser R. F., Robbins R. N., Davis J. P. A new staphylococcal enterotoxin, enterotoxin F, associated with toxic-shock-syndrome Staphylococcus aureus isolates. Lancet. 1981 May 9;1(8228):1017–1021. doi: 10.1016/s0140-6736(81)92186-3. [DOI] [PubMed] [Google Scholar]
  3. Bonventre P. F., Weckbach L., Staneck J., Schlievert P. M., Thompson M. Production of staphylococcal enterotoxin F and pyrogenic exotoxin C by Staphylococcus aureus isolates from toxic shock syndrome-associated sources. Infect Immun. 1983 Jun;40(3):1023–1029. doi: 10.1128/iai.40.3.1023-1029.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Donnelly C. B., Leslie J. E., Black L. A., Lewis K. H. Serological identification of enterotoxigenic staphylococci from cheese. Appl Microbiol. 1967 Nov;15(6):1382–1387. doi: 10.1128/am.15.6.1382-1387.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fey H., Burkhard G. Measurement of staphylococcal protein A and detection of protein A-carrying staphylococcus strains by a competitive ELISA method. J Immunol Methods. 1981;47(1):99–107. doi: 10.1016/0022-1759(81)90260-x. [DOI] [PubMed] [Google Scholar]
  6. Fey H., Pfister H., Rüegg O. Comparative evaluation of different enzyme-linked immunosorbent assay systems for the detection of staphylococcal enterotoxins A, B, C, and D. J Clin Microbiol. 1984 Jan;19(1):34–38. doi: 10.1128/jcm.19.1.34-38.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Freed R. C., Evenson M. L., Reiser R. F., Bergdoll M. S. Enzyme-linked immunosorbent assay for detection of staphylococcal enterotoxins in foods. Appl Environ Microbiol. 1982 Dec;44(6):1349–1355. doi: 10.1128/aem.44.6.1349-1355.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Garbe P. L., Arko R. J., Reingold A. L., Graves L. M., Hayes P. S., Hightower A. W., Chandler F. W., Broome C. V. Staphylococcus aureus isolates from patients with nonmenstrual toxic shock syndrome. Evidence for additional toxins. JAMA. 1985 May 3;253(17):2538–2542. [PubMed] [Google Scholar]
  9. HALLANDER H. O. PRODUCTION OF LARGE QUANTITIES OF ENTEROTOXIN B AND OTHER STAPHYLOCOCCAL TOXINS ON SOLID MEDIA. Acta Pathol Microbiol Scand. 1965;63:299–305. doi: 10.1111/apm.1965.63.2.299. [DOI] [PubMed] [Google Scholar]
  10. Igarashi H., Fujikawa H., Usami H., Kawabata S., Morita T. Purification and characterization of Staphylococcus aureus FRI 1169 and 587 toxic shock syndrome exotoxins. Infect Immun. 1984 Apr;44(1):175–181. doi: 10.1128/iai.44.1.175-181.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ikejima T., Dinarello C. A., Gill D. M., Wolff S. M. Induction of human interleukin-1 by a product of Staphylococcus aureus associated with toxic shock syndrome. J Clin Invest. 1984 May;73(5):1312–1320. doi: 10.1172/JCI111334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kauffman P. E. Enzyme immunoassay for staphylococcal enterotoxin A. J Assoc Off Anal Chem. 1980 Sep;63(5):1138–1143. [PubMed] [Google Scholar]
  13. Notermans S., Dufrenne J. B. A simple purification method for enterotoxin F produced by Staphylococcus aureus and some properties of the toxin. Antonie Van Leeuwenhoek. 1982 Dec;48(5):447–455. doi: 10.1007/BF00448416. [DOI] [PubMed] [Google Scholar]
  14. Notermans S., Timmermans P., Nagel J. Interaction of staphylococcal protein A in enzyme-linked immunosorbent assays for detecting staphylococcal antigens. J Immunol Methods. 1982 Nov 26;55(1):35–41. doi: 10.1016/0022-1759(82)90074-6. [DOI] [PubMed] [Google Scholar]
  15. Parsonnet J., Hickman R. K., Eardley D. D., Pier G. B. Induction of human interleukin-1 by toxic-shock-syndrome toxin-1. J Infect Dis. 1985 Mar;151(3):514–522. doi: 10.1093/infdis/151.3.514. [DOI] [PubMed] [Google Scholar]
  16. Reiser R. F., Robbins R. N., Khoe G. P., Bergdoll M. S. Purification and some physicochemical properties of toxic-shock toxin. Biochemistry. 1983 Aug 2;22(16):3907–3912. doi: 10.1021/bi00285a028. [DOI] [PubMed] [Google Scholar]
  17. Ritz H. L., Kirkland J. J., Bond G. G., Warner E. K., Petty G. P. Association of high levels of serum antibody to staphylococcal toxic shock antigen with nasal carriage of toxic shock antigen-producing strains of Staphylococcus aureus. Infect Immun. 1984 Mar;43(3):954–958. doi: 10.1128/iai.43.3.954-958.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Robbins R., Gould S., Bergdoll M. Detecting the enterotoxigenicity of Staphylococcus aureus strains. Appl Microbiol. 1974 Dec;28(6):946–950. doi: 10.1128/am.28.6.946-950.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Saunders G. C., Bartlett M. L. Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A. Appl Environ Microbiol. 1977 Nov;34(5):518–522. doi: 10.1128/aem.34.5.518-522.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schlievert P. M. Enhancement of host susceptibility to lethal endotoxin shock by staphylococcal pyrogenic exotoxin type C. Infect Immun. 1982 Apr;36(1):123–128. doi: 10.1128/iai.36.1.123-128.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schlievert P. M., Shands K. N., Dan B. B., Schmid G. P., Nishimura R. D. Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic-shock syndrome. J Infect Dis. 1981 Apr;143(4):509–516. doi: 10.1093/infdis/143.4.509. [DOI] [PubMed] [Google Scholar]
  22. Stiffler-Rosenberg G., Fey H. Simple assay for staphylococcal enterotoxins A, B, and C: modification of enzyme-linked immunosorbent assay. J Clin Microbiol. 1978 Nov;8(5):473–479. doi: 10.1128/jcm.8.5.473-479.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Surujballi O. P., Fackrell H. B. Enzyme-linked immunosorbent assay for detection of Staphylococcus aureus alpha-toxin. J Clin Microbiol. 1984 Mar;19(3):394–398. doi: 10.1128/jcm.19.3.394-398.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Van Miert A. S., Van Duin C. T., Schotman A. J. Comparative observations of fever and associated clinical hematological and blood biochemical changes after intravenous administration of staphylococcal enterotoxins B and F (toxic shock syndrome toxin-1) in goats. Infect Immun. 1984 Nov;46(2):354–360. doi: 10.1128/iai.46.2.354-360.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. de Azavedo J. C., Arbuthnott J. P. Toxicity of staphylococcal toxic shock syndrome toxin 1 in rabbits. Infect Immun. 1984 Nov;46(2):314–317. doi: 10.1128/iai.46.2.314-317.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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