Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1985 Oct;22(4):656–658. doi: 10.1128/jcm.22.4.656-658.1985

Rapid detection of herpes simplex virus DNA in human brain tissue by in situ hybridization.

B Forghani, K W Dupuis, N J Schmidt
PMCID: PMC268487  PMID: 3001137

Abstract

A commercial system for detection of herpes simplex virus (HSV) DNA by in situ hybridization gave positive results on 16 of 17 stored human brain specimens that were positive for HSV on initial testing by virus isolation and immunofluorescence staining, and the hybridization system gave negative results on 13 brain specimens that showed no evidence of HSV by isolation or immunofluorescence staining.

Full text

PDF
657

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brigati D. J., Myerson D., Leary J. J., Spalholz B., Travis S. Z., Fong C. K., Hsiung G. D., Ward D. C. Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. Virology. 1983 Apr 15;126(1):32–50. doi: 10.1016/0042-6822(83)90460-9. [DOI] [PubMed] [Google Scholar]
  2. Langer P. R., Waldrop A. A., Ward D. C. Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6633–6637. doi: 10.1073/pnas.78.11.6633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Leary J. J., Brigati D. J., Ward D. C. Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Proc Natl Acad Sci U S A. 1983 Jul;80(13):4045–4049. doi: 10.1073/pnas.80.13.4045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Richman D. D., Cleveland P. H., Redfield D. C., Oxman M. N., Wahl G. M. Rapid viral diagnosis. J Infect Dis. 1984 Mar;149(3):298–310. doi: 10.1093/infdis/149.3.298. [DOI] [PubMed] [Google Scholar]
  5. Richman D., Schmidt N., Plotkin S., Yolken R., Cherensky M., McIntosh K., Mattheis M. Summary of a workshop on new and useful methods in rapid viral diagnosis. J Infect Dis. 1984 Dec;150(6):941–951. doi: 10.1093/infdis/150.6.941. [DOI] [PubMed] [Google Scholar]
  6. Schmidt N. J., Gallo D., Devlin V., Woodie J. D., Emmons R. W. Direct immunofluorescence staining for detection of herpes simplex and varicella-zoster virus antigens in vesicular lesions and certain tissue specimens. J Clin Microbiol. 1980 Nov;12(5):651–655. doi: 10.1128/jcm.12.5.651-655.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES