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. 1985 Nov;22(5):740–744. doi: 10.1128/jcm.22.5.740-744.1985

Rapid diagnosis of LaCrosse encephalitis: detection of specific immunoglobulin M in cerebrospinal fluid.

T I Dykers, K L Brown, C B Gundersen, B J Beaty
PMCID: PMC268517  PMID: 3902876

Abstract

An immunoglobulin M (IgM) antibody capture enzyme immunoassay (MAC-EIA) was developed for the rapid and early diagnosis of LaCrosse (LAC) virus infections. The MAC-EIA was a sensitive and specific technique for the detection of IgM antibodies to LAC virus in cerebrospinal fluid specimens and in acute-phase serum specimens. In a retrospective study, cerebrospinal fluid and acute-phase serum paired samples from 108 patients were tested by the MAC-EIA and by an IgM immunofluorescence assay. The results were compared with the original diagnosis, which was made by using a variety of classical serological tests including serum neutralization, hemagglutination inhibition, and complement fixation. Thirty patients were confirmed as having LAC virus infections; of these, 30 (100%) were diagnosed as positive by serum MAC-EIA, and 27 (90%) were positive by cerebrospinal fluid MAC-EIA. The MAC-EIA was more sensitive than the IgM immunofluorescence assay. Two patients who were not previously confirmed as positive cases were diagnosed as having LAC virus infections by the MAC-EIA. One patient who was subsequently diagnosed as having a Jamestown Canyon virus infection and two patients who were previously infected with Jamestown Canyon virus were not falsely identified as having LAC virus infections by the MAC-EIA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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