Abstract
A halophilic gram-negative rod was isolated from blood and cerebrospinal fluid collected from a 70-year-old male having no known contact with seafood or salt water. Positive biochemical tests included oxidase, sensitivity to 0/129, O-nitrophenyl-beta-D-galactopyranoside, lysine decarboxylase and fermentation of glucose, salicin, n-inositol, sucrose, L-mannose, L-arabinose, and arbutin. Negative tests included indole, ornithine decarboxylase, arginine dihydrolase fermentation of lactose, and production of gelatinase and urease. The DNA base composition was 45.0 mol% guanine plus cytosine. Numerical taxonomy indicated 70% similarity with known reference Vibrio sp. strains. The 5S rRNA sequence for this strain has been determined: 5'-U G C C U G G C G A C C A U A G C G U U U U G G A C C C A C C U G A U U C C A U G C C G A A C U C A G U A G U G A A A C G A A A C A G C G U C G A U G G U A G U G U G G G G U C U C C C C A U G U G A G A G U A G A A C A U C G C C A G G C A U-3'. Based on the phenetic, molecular genetic, and nucleic acid sequencing data, it is concluded that Vibrio cincinnatiensis represents a new species of the genus Vibrio sensu strictu (as defined by 5S rRNA sequencing results). On a basis of 5S rRNA comparative sequence analysis, the organism appears to share a recent common ancestor with V. gazogenes (98% homology) and close ancestry with V. mimicus, V. fluvialis, and V. metschnikovii.
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- Billy C. Etude d'une bactérie alginolytique anaérobie: Clostridium alginolyticum n. sp. Ann Inst Pasteur (Paris) 1965 Jul;109(1):147–151. [PubMed] [Google Scholar]
- Deming J. W., Colwell R. R. Barophilic bacteria associated with digestive tracts of abyssal holothurians. Appl Environ Microbiol. 1982 Nov;44(5):1222–1230. doi: 10.1128/aem.44.5.1222-1230.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fitch W. M., Margoliash E. Construction of phylogenetic trees. Science. 1967 Jan 20;155(3760):279–284. doi: 10.1126/science.155.3760.279. [DOI] [PubMed] [Google Scholar]
- Guerinot M. L., Patriquin D. G. N2-fixing vibrios isolated from the gastrointestinal tract of sea urchins. Can J Microbiol. 1981 Mar;27(3):311–317. doi: 10.1139/m81-048. [DOI] [PubMed] [Google Scholar]
- Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980 Dec;16(2):111–120. doi: 10.1007/BF01731581. [DOI] [PubMed] [Google Scholar]
- Kodaka H., Armfield A. Y., Lombard G. L., Dowell V. R., Jr Practical procedure for demonstrating bacterial flagella. J Clin Microbiol. 1982 Nov;16(5):948–952. doi: 10.1128/jcm.16.5.948-952.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWE G. H. The rapid detection of lactose fermentation in paracolon organisms by the demonstration of beta-D-galactosidase. J Med Lab Technol. 1962 Jan;19:21–25. [PubMed] [Google Scholar]
- MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
- MacDonell M. T., Colwell R. R. Identical 5S rRNA nucleotide sequence of Vibrio cholerae strains representing temporal, geographical, and ecological diversity. Appl Environ Microbiol. 1984 Jul;48(1):119–121. doi: 10.1128/aem.48.1.119-121.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MacDonell M. T., Colwell R. R. Nucleotide base sequence of vibrionaceae 5 S rRNA. FEBS Lett. 1984 Sep 17;175(1):183–188. doi: 10.1016/0014-5793(84)80595-5. [DOI] [PubMed] [Google Scholar]