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. 1986 May;23(5):881–886. doi: 10.1128/jcm.23.5.881-886.1986

Production and characterization of agglutinating monoclonal antibodies against predominant antigenic factors for Candida albicans.

Y Miyakawa, K Kagaya, Y Fukazawa, G Soe
PMCID: PMC268742  PMID: 3519660

Abstract

Two clones, CA4-2 and CA5-4, which produced agglutinating monoclonal immunoglobulin M (IgM) antibodies (MAbs) against mannan antigens of Candida albicans serotype A, were established. The specificity of each MAb was determined by slide agglutination tests for cross-reactivity patterns against the homologous and six other strains of Candida and a strain of Torulopsis: C. albicans serotype B, C. tropicalis, C. guilliermondii, C. krusei, C. parapsilosis, C. pseudotropicalis, and Torulopsis glabrata. The MAb produced by CA4-2 reacted with the homologous, C. tropicalis, and T. glabrata strains, whereas the MAb produced by CA5-4 reacted with the homologous, C. albicans serotype B, and C. tropicalis strains. These results are consistent with results obtained by comparative experiments with several strains of each serotype or species. Specificity of these two MAbs by agglutination was also consistent with the cross-reactivity patterns demonstrated by indirect immunofluorescence staining. The competitive binding experiments by immunofluorescence staining with two MAbs and polyclonal factor sera (PAb factors) 5 and 6 suggested that the MAb from clone CA4-2 did not completely correspond to PAb factor 6 and that the MAb from CA5-4 was distinct from PAb factor 5 in its manner of binding to determinants (the latter was designated 5b), Cross-reactivity patterns, however, furnished evidence that these two MAbs could replace the known PAb factors 6 and 5, respectively, as reagents for aid in the identification of the strains of C. albicans and their serotypes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashall F., Bramwell M. E., Harris H. A new marker for human cancer cells. 1 The Ca antigen and the Ca1 antibody. Lancet. 1982 Jul 3;2(8288):1–6. doi: 10.1016/s0140-6736(82)91150-3. [DOI] [PubMed] [Google Scholar]
  2. Ballou C. E., Raschke W. C. Polymorphism of the somatic antigen of yeast. Science. 1974 Apr 12;184(4133):127–134. doi: 10.1126/science.184.4133.127. [DOI] [PubMed] [Google Scholar]
  3. Brawner D. L., Cutler J. E. Variability in expression of a cell surface determinant on Candida albicans as evidenced by an agglutinating monoclonal antibody. Infect Immun. 1984 Mar;43(3):966–972. doi: 10.1128/iai.43.3.966-972.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fukazawa Y., Shinoda T., Tsuchiya T. Response and specificity of antibodies for Candida albicans. J Bacteriol. 1968 Mar;95(3):754–763. doi: 10.1128/jb.95.3.754-763.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fukazawa Y., Tsuchiya T. Immunological structure and specificity of the cell wall of yeasts. Antonie Van Leeuwenhoek. 1969 Jun;35(Suppl):E7–E8. [PubMed] [Google Scholar]
  6. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  7. Gustafsson B., Rosén A., Holme T. Monoclonal antibodies against Vibrio cholerae lipopolysaccharide. Infect Immun. 1982 Nov;38(2):449–454. doi: 10.1128/iai.38.2.449-454.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HASENCLEVER H. F., MITCHELL W. O. Antigenic studies of Candida. I. Observation of two antigenic groups in Candida albicans. J Bacteriol. 1961 Oct;82:570–573. doi: 10.1128/jb.82.4.570-573.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kirkland T. N., Ziegler E. J. An immunoprotective monoclonal antibody to lipopolysaccharide. J Immunol. 1984 May;132(5):2590–2592. [PubMed] [Google Scholar]
  10. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  11. Lehmann P. F., Reiss E. Comparison by ELISA of serum anti-Candida albicans mannan IgG levels of a normal population and in diseased patients. Mycopathologia. 1980 Mar 17;70(2):89–93. doi: 10.1007/BF00443073. [DOI] [PubMed] [Google Scholar]
  12. Makinodan T., Peterson W. J. Further studies on the secondary antibody-forming potential of juvenile, young adult, adult, and aged mice. Dev Biol. 1966 Aug;14(1):112–129. doi: 10.1016/0012-1606(66)90008-x. [DOI] [PubMed] [Google Scholar]
  13. Nelles M. J., Niswander C. A., Karakawa W. W., Vann W. F., Arbeit R. D. Reactivity of type-specific monoclonal antibodies with Staphylococcus aureus clinical isolates and purified capsular polysaccharide. Infect Immun. 1985 Jul;49(1):14–18. doi: 10.1128/iai.49.1.14-18.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reiss E., Huppert M., Cherniak R. Characterization of protein and mannan polysaccharide antigens of yeasts, moulds, and actinomycetes. Curr Top Med Mycol. 1985;1:172–207. doi: 10.1007/978-1-4613-9547-8_7. [DOI] [PubMed] [Google Scholar]
  15. Sciortino C. V., Yang Z. S., Finkelstein R. A. Monoclonal antibodies to outer membrane antigens of Vibrio cholerae. Infect Immun. 1985 Jul;49(1):122–131. doi: 10.1128/iai.49.1.122-131.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shinoda T., Kaufman L., Padhye A. A. Comparative evaluation of the Iatron serological Candida check kit and the API 20C kit for identification of medically important Candida species. J Clin Microbiol. 1981 Mar;13(3):513–518. doi: 10.1128/jcm.13.3.513-518.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Springer T., Galfré G., Secher D. S., Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979 Apr;9(4):301–306. doi: 10.1002/eji.1830090410. [DOI] [PubMed] [Google Scholar]
  18. Suzuki M., Fukazawa Y. Immunochemical characterization of Candida albicans cell wall antigens: specific determinant of Candida albicans serotype A mannan. Microbiol Immunol. 1982;26(5):387–402. doi: 10.1111/j.1348-0421.1982.tb00189.x. [DOI] [PubMed] [Google Scholar]
  19. Tsuchiya T., Fukazawa Y., Kawakita S. Significance of serological studies on yeasts. Mycopathol Mycol Appl. 1965 Jun 15;26(1):1–15. doi: 10.1007/BF02098585. [DOI] [PubMed] [Google Scholar]
  20. Winterrowd G. E., Cutler J. E. Candida albicans-induced agglutinin and immunoglobulin E responses in mice. Infect Immun. 1983 Jul;41(1):33–38. doi: 10.1128/iai.41.1.33-38.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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