Abstract
The activities of cefotaxime (CTX) and desacetyl cefotaxime (des-CTX) were tested both singly and in combination against 173 anaerobic clinical isolates. The MIC of CTX for 50% of 60 Bacteroides fragilis isolates was 22.4 micrograms/ml in broth, compared with 47.4 micrograms/ml in agar. This reduced efficacy in agar was seen with all species tested and is in apparent conflict with reported clinical efficacy of the drug. Synergy between CTX and des-CTX was observed with 70 to 100% of the isolates, including 60% of all Bacteroides spp. tested. The susceptibility results in a synergy system correlated well with those noted in a broth-disk elution method incorporating 32 micrograms of CTX and 8 micrograms of des-CTX per ml. The correlation was poorer when the broth-disk method contained 16 micrograms of CTX and 8 micrograms of des-CTX per ml.
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Selected References
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- Doluisio J. T. Clinical pharmacokinetics of cefotaxime in patients with normal and reduced renal function. Rev Infect Dis. 1982 Sep-Oct;4 (Suppl):S333–S345. doi: 10.1093/clinids/4.supplement_2.s333. [DOI] [PubMed] [Google Scholar]
- ELION G. B., SINGER S., HITCHINGS G. H. Antagonists of nucleic acid derivatives. VIII. Synergism in combinations of biochemically related antimetabolites. J Biol Chem. 1954 Jun;208(2):477–488. [PubMed] [Google Scholar]
- GARROD L. P., WATERWORTH P. M. Methods of testing combined antibiotic bactericidal action and the significance of the results. J Clin Pathol. 1962 Jul;15:328–338. doi: 10.1136/jcp.15.4.328. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones R. N., Barry A. L., Thornsberry C. Antimicrobial activity of desacetylcefotaxime alone and in combination with cefotaxime: evidence of synergy. Rev Infect Dis. 1982 Sep-Oct;4 (Suppl):S366–S373. doi: 10.1093/clinids/4.supplement_2.s366. [DOI] [PubMed] [Google Scholar]
- Neu H. C. Antibacterial activity of desacetylcefotaxime alone and in combination with cefotaxime. Rev Infect Dis. 1982 Sep-Oct;4 (Suppl):S374–S378. doi: 10.1093/clinids/4.supplement_2.s374. [DOI] [PubMed] [Google Scholar]
- Novick W. J., Jr Levels of cefotaxime in body fluids and tissues: a review. Rev Infect Dis. 1982 Sep-Oct;4 (Suppl):S346–S353. doi: 10.1093/clinids/4.supplement_2.s346. [DOI] [PubMed] [Google Scholar]
- Stone H. H., Morris E. S., Geheber C. E., Kolb L. D., Dunlop W. E. Clinical comparison of cefotaxime with gentamicin plus clindamycin in the treatment of peritonitis and other soft-tissue infections. Rev Infect Dis. 1982 Sep-Oct;4 (Suppl):S439–S443. doi: 10.1093/clinids/4.supplement_2.s439. [DOI] [PubMed] [Google Scholar]
- Stone H. H., Morris E. S., Geheber C. E., Kolb L. D., Dunlop W. E. Clinical comparison of cefotaxime with gentamicin plus clindamycin in the treatment of peritonitis and other soft-tissue infections. Rev Infect Dis. 1982 Sep-Oct;4 (Suppl):S439–S443. doi: 10.1093/clinids/4.supplement_2.s439. [DOI] [PubMed] [Google Scholar]
- Wilkins T. D., Thiel T. Modified broth-disk method for testing the antibiotic susceptibility of anaerobic bacteria. Antimicrob Agents Chemother. 1973 Mar;3(3):350–356. doi: 10.1128/aac.3.3.350. [DOI] [PMC free article] [PubMed] [Google Scholar]