Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Sep;24(3):377–379. doi: 10.1128/jcm.24.3.377-379.1986

Isolation of Mycoplasma species and Ureaplasma urealyticum from obstetrical and gynecological patients by using commercially available medium formulations.

L E Phillips, K H Goodrich, R M Turner, S Faro
PMCID: PMC268917  PMID: 3760133

Abstract

One hundred duplicate endocervical specimens from obstetrical and gynecological patients were cultured for Mycoplasma spp. and Ureaplasma urealyticum. Rates of recovery of these organisms from commercially prepared A7 medium and the Mycotrim-GU system were compared. We detected 14 (93%) of the total 15 isolates of Mycoplasma spp. on A7 plates and 11 (73%) in the Mycotrim-GU system. We detected 34 (89%) of the total 38 isolates of U. urealyticum on A7 plates and 32 (84%) in the Mycotrim-GU system. The times of detection for both types of organism were similar in the two systems. We conclude that cultivation on A7 medium as described is a more cost-effective method of recovery of Mycoplasma spp. and U. urealyticum than the Mycotrim-GU system.

Full text

PDF
378

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blanco J. D., Gibbs R. S., Malherbe H., Strickland-Cholmley M., St Clair P. J., Castaneda Y. S. A controlled study of genital mycoplasmas in amniotic fluid from patients with intra-amniotic infection. J Infect Dis. 1983 Apr;147(4):650–653. doi: 10.1093/infdis/147.4.650. [DOI] [PubMed] [Google Scholar]
  2. Cassell G. H., Cole B. C. Mycoplasmas as agents of human disease. N Engl J Med. 1981 Jan 8;304(2):80–89. doi: 10.1056/NEJM198101083040204. [DOI] [PubMed] [Google Scholar]
  3. Fiacco V., Miller M. J., Carney E., Martin W. J. Comparison of media for isolation of Ureaplasma urealyticum and genital Mycoplasma species. J Clin Microbiol. 1984 Nov;20(5):862–865. doi: 10.1128/jcm.20.5.862-865.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gibbs R. S., Blanco J. D., St Clair P. J., Castaneda Y. S. Mycoplasma hominis and intrauterine infection in late pregnancy. Sex Transm Dis. 1983 Oct-Dec;10(4 Suppl):303–306. [PubMed] [Google Scholar]
  5. Kundsin R. B., Driscoll S. G., Monson R. R., Yeh C., Biano S. A., Cochran W. D. Association of Ureaplasma urealyticum in the placenta with perinatal morbidity and mortality. N Engl J Med. 1984 Apr 12;310(15):941–945. doi: 10.1056/NEJM198404123101502. [DOI] [PubMed] [Google Scholar]
  6. Kundsin R. B., Driscoll S. G., Pelletier P. A. Ureaplasma urealyticum incriminated in perinatal morbidity and mortality. Science. 1981 Jul 24;213(4506):474–475. doi: 10.1126/science.7244646. [DOI] [PubMed] [Google Scholar]
  7. Kundsin R. B., Parreno A., Poulin S. Significance of appropriate techniques and media for isolation and identification of Ureaplasma urealyticum from clinical specimens. J Clin Microbiol. 1978 Oct;8(4):445–453. doi: 10.1128/jcm.8.4.445-453.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Leland D. S., Lapworth M. A., Jones R. B., French M. L. Comparative evaluation of media for isolation of ureaplasma urealyticum and genital mycoplasma species. J Clin Microbiol. 1982 Oct;16(4):709–714. doi: 10.1128/jcm.16.4.709-714.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McCormack W. M., Lee Y. H., Lin J. S., Rankin J. S. Genital mycoplasmas in postpartum fever. J Infect Dis. 1973 Feb;127(2):193–196. doi: 10.1093/infdis/127.2.193. [DOI] [PubMed] [Google Scholar]
  10. McCormack W. M., Rosner B., Lee Y. H., Rankin J. S., Lin J. S. Isolation of genital mycoplasmas from blood obtained shortly after vaginal delivery. Lancet. 1975 Mar 15;1(7907):596–599. doi: 10.1016/s0140-6736(75)91881-4. [DOI] [PubMed] [Google Scholar]
  11. Mårdh P. A., Weström L. Antibodies to Mycoplasma hominis in patients with genital infections and in healthy controls. Br J Vener Dis. 1970 Oct;46(5):390–397. [PMC free article] [PubMed] [Google Scholar]
  12. Møller B. R. The role of mycoplasmas in the upper genital tract of women. Sex Transm Dis. 1983 Oct-Dec;10(4 Suppl):281–284. [PubMed] [Google Scholar]
  13. Shepard M. C., Lunceford C. D. Differential agar medium (A7) for identification of Ureaplasma urealyticum (human T mycoplasmas) in primary cultures of clinical material. J Clin Microbiol. 1976 Jun;3(6):613–625. doi: 10.1128/jcm.3.6.613-625.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stamm W. E., Running K., Hale J., Holmes K. K. Etiologic role of Mycoplasma hominis and Ureaplasma urealyticum in women with the acute urethral syndrome. Sex Transm Dis. 1983 Oct-Dec;10(4 Suppl):318–322. [PubMed] [Google Scholar]
  15. Taylor-Robinson D., McCormack W. M. The genital mycoplasmas (first of two parts). N Engl J Med. 1980 May 1;302(18):1003–1010. doi: 10.1056/NEJM198005013021805. [DOI] [PubMed] [Google Scholar]
  16. Taylor-Robinson D., McCormack W. M. The genital mycoplasmas (second of two parts). N Engl J Med. 1980 May 8;302(19):1063–1067. doi: 10.1056/NEJM198005083021905. [DOI] [PubMed] [Google Scholar]
  17. Wallace R. J., Jr, Alpert S., Browne K., Lin J. S., McCormack W. M. Isolation of Mycoplasma hominis from blood cultures in patients with postpartum fever. Obstet Gynecol. 1978 Feb;51(2):181–185. [PubMed] [Google Scholar]
  18. Wood J. C., Lu R. M., Peterson E. M., de la Maza L. M. Evaluation of Mycotrim-GU for isolation of Mycoplasma species and Ureaplasma urealyticum. J Clin Microbiol. 1985 Nov;22(5):789–792. doi: 10.1128/jcm.22.5.789-792.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yajko D. M., Balston E., Wood D., Sweet R. L., Hadley W. K. Evaluation of PPLO, A7B, E, and NYC agar media for the isolation of Ureaplasma urealyticum and Mycoplasma species from the genital tract. J Clin Microbiol. 1984 Jan;19(1):73–76. doi: 10.1128/jcm.19.1.73-76.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES