Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Sep;24(3):487–489. doi: 10.1128/jcm.24.3.487-489.1986

Viral isolation versus immune staining of infected cell cultures for the laboratory diagnosis of herpes simplex virus infections.

J H Hughes, D R Mann, V V Hamparian
PMCID: PMC268946  PMID: 3020087

Abstract

The Difco Laboratories Cellmatics kit, an immune staining method for the detection of herpes simplex virus after amplification in culture, was found to be 94.6% (138 of 146) sensitive and 100% (302 of 302) specific. However, conventional culturing detected 10 (6.4%) additional viruses that were not herpes simplex. For a full-service diagnostic laboratory, viral isolation is important.

Full text

PDF
488

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benjamin D. R., Ray C. G. Use of immunoperoxidase on brain tissue for the rapid diagnosis of herpes encephalitis. Am J Clin Pathol. 1975 Oct;64(4):472–476. doi: 10.1093/ajcp/64.4.472. [DOI] [PubMed] [Google Scholar]
  2. Benjamin D. R. Use of immunoperoxidase for rapid diagnosis of mucocutaneous herpes simplex virus infection. J Clin Microbiol. 1977 Dec;6(6):571–573. doi: 10.1128/jcm.6.6.571-573.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Callihan D. R., Menegus M. A. Rapid detection of herpes simplex virus in clinical specimens with human embryonic lung fibroblast and primary rabbit kidney cell cultures. J Clin Microbiol. 1984 Apr;19(4):563–565. doi: 10.1128/jcm.19.4.563-565.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fayram S. L., Aarnaes S., de la Maza L. M. Comparison of cultureset to a conventional tissue culture-fluorescent-antibody technique for isolation and identification of herpes simplex virus. J Clin Microbiol. 1983 Jul;18(1):215–216. doi: 10.1128/jcm.18.1.215-216.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hayden F. G., Sorensen A. S., Bateman J. A. Comparison of the Immulok cultureset kit and virus isolation for detection of herpes simplex virus in clinical specimens. J Clin Microbiol. 1983 Jul;18(1):222–224. doi: 10.1128/jcm.18.1.222-224.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Herrmann E. C., Jr Experiences in laboratory diagnosis of herpes simplex, varicella-zoster, and vaccinia virus infections in routine medical practice. Mayo Clin Proc. 1967 Nov;42(11):744–753. [PubMed] [Google Scholar]
  7. Johnson F. B., Leavitt R. W., Richards D. F. Comparison of the Scott Selecticult-HSV kit with conventional culture and direct immunoperoxidase staining for detection of herpes simplex virus in cultures of clinical specimens. J Clin Microbiol. 1985 Mar;21(3):438–441. doi: 10.1128/jcm.21.3.438-441.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Johnson F. B., Leavitt R. W., Richards D. F. Evaluation of the virocult transport tube for isolation of herpes simplex virus from clinical specimens. J Clin Microbiol. 1984 Jul;20(1):120–122. doi: 10.1128/jcm.20.1.120-122.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Landry M. L., Mayo D. R., Hsiung G. D. Comparison of guinea pig embryo cells, rabbit kidney cells, and human embryonic lung fibroblast cell strains for isolation of herpes simplex virus. J Clin Microbiol. 1982 May;15(5):842–847. doi: 10.1128/jcm.15.5.842-847.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mayo D. R., Brennan T., Egbertson S. H., Moore D. F. Rapid herpes simplex virus detection in clinical samples submitted to a state virology laboratory. J Clin Microbiol. 1985 May;21(5):768–771. doi: 10.1128/jcm.21.5.768-771.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Miller M. J., Howell C. L. Rapid detection and identification of herpes simplex virus in cell culture by a direct immunoperoxidase staining procedure. J Clin Microbiol. 1983 Sep;18(3):550–553. doi: 10.1128/jcm.18.3.550-553.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Morgan M. A., Smith T. F. Evaluation of an enzyme-linked immunosorbent assay for the detection of herpes simplex virus antigen. J Clin Microbiol. 1984 Jun;19(6):730–732. doi: 10.1128/jcm.19.6.730-732.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moseley R. C., Corey L., Benjamin D., Winter C., Remington M. L. Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection. J Clin Microbiol. 1981 May;13(5):913–918. doi: 10.1128/jcm.13.5.913-918.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pearson N. S., Fleagle G., Docherty J. J. Detection of herpes simplex virus infection of female genitalia by the peroxidase-antiperoxidase method alone or in conjunction with the Papanicolaou stain. J Clin Microbiol. 1979 Nov;10(5):737–746. doi: 10.1128/jcm.10.5.737-746.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Phillips L. E., Magliolo R. A., Stehlik M. L., Whiteman P. A., Faro S., Rogers T. E. Retrospective evaluation of the isolation and identification of herpes simplex virus with Cultureset and human fibroblasts. J Clin Microbiol. 1985 Aug;22(2):255–258. doi: 10.1128/jcm.22.2.255-258.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rubin S. J., Rogers S. Comparison of Cultureset and primary rabbit kidney cell culture for the detection of herpes simplex virus. J Clin Microbiol. 1984 Jun;19(6):920–922. doi: 10.1128/jcm.19.6.920-922.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schmidt N. J., Dennis J., Devlin V., Gallo D., Mills J. Comparison of direct immunofluorescence and direct immunoperoxidase procedures for detection of herpes simplex virus antigen in lesion specimens. J Clin Microbiol. 1983 Aug;18(2):445–448. doi: 10.1128/jcm.18.2.445-448.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sewell D. L., Horn S. A., Dilbeck P. W. Comparison of Cultureset and Bartels Immunodiagnostics with conventional tissue culture for isolation and identification of herpes simplex virus. J Clin Microbiol. 1984 May;19(5):705–706. doi: 10.1128/jcm.19.5.705-706.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES