Abstract
The small asaccharolytic, nonpigmenting gram-negative rods of the human oral cavity are difficult to differentiate from each other. Protein profiles of sonicated cells of Wolinella species, Campylobacter concisus, Bacteroides gracilis, and Eikenella corrodens were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized with a silver stain. The gels were scanned with a laser densitometer, and the similarity of strains was computed by determining correlation coefficients of normalized densities along the gels. The strains were grouped by cluster analysis of the correlation coefficients. All species were distinct from each other. Several groups were found within E. corrodens. A colored silver stain was found to highlight species differences and appears to be useful in the rapid identification of fresh isolates.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dzink J. L., Tanner A. C., Haffajee A. D., Socransky S. S. Gram negative species associated with active destructive periodontal lesions. J Clin Periodontol. 1985 Sep;12(8):648–659. doi: 10.1111/j.1600-051x.1985.tb00936.x. [DOI] [PubMed] [Google Scholar]
- Johnson C. C., Reinhardt J. F., Edelstein M. A., Mulligan M. E., George W. L., Finegold S. M. Bacteroides gracilis, an important anaerobic bacterial pathogen. J Clin Microbiol. 1985 Nov;22(5):799–802. doi: 10.1128/jcm.22.5.799-802.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kersters K., De Ley J. Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patterns. J Gen Microbiol. 1975 Apr;87(2):333–342. doi: 10.1099/00221287-87-2-333. [DOI] [PubMed] [Google Scholar]
- Moore W. E., Hash D. E., Holdeman L. V., Cato E. P. Polyacrylamide slab gel electrophoresis of soluble proteins for studies of bacterial floras. Appl Environ Microbiol. 1980 Apr;39(4):900–907. doi: 10.1128/aem.39.4.900-907.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schoenle E. J., Adams L. D., Sammons D. W. Insulin-induced rapid decrease of a major protein in fat cell plasma membranes. J Biol Chem. 1984 Oct 10;259(19):12112–12116. [PubMed] [Google Scholar]
- Tanner A. C., Socransky S. S., Goodson J. M. Microbiota of periodontal pockets losing crestal alveolar bone. J Periodontal Res. 1984 May;19(3):279–291. doi: 10.1111/j.1600-0765.1984.tb00819.x. [DOI] [PubMed] [Google Scholar]