Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Dec;24(6):1038–1040. doi: 10.1128/jcm.24.6.1038-1040.1986

Rapid diagnosis of respiratory syncytial virus infection by antigen immunofluorescence detection with monoclonal antibodies and immunoglobulin M immunofluorescence test.

Z Kadi, S Dali, S Bakouri, A Bouguermouh
PMCID: PMC269094  PMID: 3536995

Abstract

During a respiratory syncytial virus (RSV) infection outbreak in a pediatric hospital, diagnosis was made by immunofluorescence on smears by using an anti-RSV monoclonal antibody (IFm). Immunoglobulins M and G were titrated by indirect immunofluorescence on HEp-2 cells infected with an RSV strain. The IFm was sensitive (89%) and specific (75%) when compared with the cell culture method. We showed that the specimens which were found positive by IFm and negative by cell culture were truly positive. Under these conditions, the IFm test appears more sensitive and more specific than cell culture, particularly when no care is taken to maintain the specimens in the cold during transport. In this study the immunoglobulin M immunofluorescence test had a low sensitivity (34%), especially on serum samples taken on days 0 to 4 after the onset of illness.

Full text

PDF
1038

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell D. M., Walsh E. E., Hruska J. F., Schnabel K. C., Hall C. B. Rapid detection of respiratory syncytial virus with a monoclonal antibody. J Clin Microbiol. 1983 Jun;17(6):1099–1101. doi: 10.1128/jcm.17.6.1099-1101.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fulton R. E., Middleton P. J. Comparison of immunofluorescence and isolation techniques in the diagnosis of respiratory viral infections of children. Infect Immun. 1974 Jul;10(1):92–101. doi: 10.1128/iai.10.1.92-101.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gallo D., Riggs J. L., Schachter J., Emmons R. W. Multiple-antigen slide test for detection of immunoglobulin M antibodies in newborn and infant sera by immunofluorescence. J Clin Microbiol. 1981 Apr;13(4):631–636. doi: 10.1128/jcm.13.4.631-636.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gardner P. S., McQuillin J., McGuckin R. The late detection of respiratory syncytial virus in cells of respiratory tract by immunofluorescence. J Hyg (Lond) 1970 Dec;68(4):575–580. doi: 10.1017/s0022172400042509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gould E. A., Allan G. M., Logan E. F., McFerran J. B. Detection of antibody to bovine syncytial virus and respiratory syncytial virus in bovine fetal serum. J Clin Microbiol. 1978 Aug;8(2):233–237. doi: 10.1128/jcm.8.2.233-237.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hornsleth A., Friis B., Grauballe P. C., Krasilnikof P. A. Detection by ELISA of IgA and IgM antibodies in secretion and IgM antibodies in serum in primary lower respiratory syncytial virus infection. J Med Virol. 1984;13(2):149–161. doi: 10.1002/jmv.1890130205. [DOI] [PubMed] [Google Scholar]
  7. Kaul T. N., Welliver R. C., Ogra P. L. Comparison of fluorescent-antibody, neutralizing-antibody, and complement-enhanced neutralizing-antibody assays for detection of serum antibody to respiratory syncytial virus. J Clin Microbiol. 1981 May;13(5):957–962. doi: 10.1128/jcm.13.5.957-962.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kim H. W., Wyatt R. G., Fernie B. F., Brandt C. D., Arrobio J. O., Jeffries B. C., Parrott R. H. Respiratory syncytial virus detection by immunofluorescence in nasal secretions with monoclonal antibodies against selected surface and internal proteins. J Clin Microbiol. 1983 Dec;18(6):1399–1404. doi: 10.1128/jcm.18.6.1399-1404.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Minnich L. L., Ray C. G. Comparison of direct and indirect immunofluorescence staining of clinical specimens for detection of respiratory syncytial virus antigen. J Clin Microbiol. 1982 May;15(5):969–970. doi: 10.1128/jcm.15.5.969-970.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Minnich L., Ray C. G. Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques. J Clin Microbiol. 1980 Sep;12(3):391–394. doi: 10.1128/jcm.12.3.391-394.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Orstavik I., Grandien M., Halonen P., Arstila P., Mordhorst C. H., Hornsleth A., Popow-Kraupp T., McQuillin J., Gardner P. S., Almeida J. Viral diagnoses using the rapid immunofluorescence technique and epidemiological implications of acute respiratory infections among children in different European countries. Bull World Health Organ. 1984;62(2):307–313. [PMC free article] [PubMed] [Google Scholar]
  12. Pothier P., Nicolas J. C., de Saint Maur G. P., Ghim S., Kazmierczak A., Bricout F. Monoclonal antibodies against respiratory syncytial virus and their use for rapid detection of virus in nasopharyngeal secretions. J Clin Microbiol. 1985 Feb;21(2):286–287. doi: 10.1128/jcm.21.2.286-287.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Treuhaft M. W., Soukup J. M., Sullivan B. J. Practical recommendations for the detection of pediatric respiratory syncytial virus infections. J Clin Microbiol. 1985 Aug;22(2):270–273. doi: 10.1128/jcm.22.2.270-273.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Welliver R. C., Kaul T. N., Putnam T. I., Sun M., Riddlesberger K., Ogra P. L. The antibody response to primary and secondary infection with respiratory syncytial virus: kinetics of class-specific responses. J Pediatr. 1980 May;96(5):808–813. doi: 10.1016/s0022-3476(80)80547-6. [DOI] [PubMed] [Google Scholar]
  15. Wong D. T., Welliver R. C., Riddlesberger K. R., Sun M. S., Ogra P. L. Rapid diagnosis of parainfluenza virus infection in children. J Clin Microbiol. 1982 Jul;16(1):164–167. doi: 10.1128/jcm.16.1.164-167.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES