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. 1987 Nov;25(11):2094–2097. doi: 10.1128/jcm.25.11.2094-2097.1987

Genetic and antigenic differences of serologically indistinguishable leptospires of serovar hardjo.

R B LeFebvre 1, A B Thiermann 1, J Foley 1
PMCID: PMC269418  PMID: 2447118

Abstract

Pathogenic leptospires of serovar hardjo isolated from North American cattle were compared genetically and antigenically to reference strain hardjoprajitno of the Sejroe serogroup. Guanine-plus-cytosine (G+C) contents were determined for the genomes, and microscopic agglutination, Western blotting (immunoblotting), and immunoprecipitation were used to characterize antigenic relatedness. Major differences were demonstrated between the isolates and the reference strain. The G+C content of the reference strain was calculated to be approximately 34 +/- 1%, and those of the isolates were calculated at 39 +/- 1%. Antigenic differences between the isolates and the reference strain were identified by using rabbit immune serum raised against a hardjo isolate exhaustively adsorbed with hardjoprajitno whole and sonicated cells. Western blot analysis and immunoprecipitation using this adsorbed serum revealed antigens apparently unique for the hardjo isolates. Microscopic agglutination with the adsorbed rabbit serum did not agglutinate hardjoprajitno when diluted 1:2 but agglutinated bovine isolates to a 1:32 dilution. Bovine antiserum raised against the isolates was also used to identify antigens by immunoprecipitation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  2. Cole J. R., Jr, Sulzer C. R., Pursell A. R. Improved microtechnique for the leptospiral microscopic agglutination test. Appl Microbiol. 1973 Jun;25(6):976–980. doi: 10.1128/am.25.6.976-980.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  4. Le Febvre R. B., Thiermann A. B. DNA homology studies of leptospires of serogroups Sejroe and Pomona from cattle and swine. Am J Vet Res. 1986 Apr;47(4):959–963. [PubMed] [Google Scholar]
  5. Marshall R. B., Wilton B. E., Robinson A. J. Identification of Leptospira serovars by restriction-endonuclease analysis. J Med Microbiol. 1981 Feb;14(1):163–166. doi: 10.1099/00222615-14-1-163. [DOI] [PubMed] [Google Scholar]
  6. Nunes-Edwards P. L., Thiermann A. B., Bassford P. J., Jr, Stamm L. V. Identification and characterization of the protein antigens of Leptospira interrogans serovar hardjo. Infect Immun. 1985 May;48(2):492–497. doi: 10.1128/iai.48.2.492-497.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Schmerr M. J., Patterson J. M., Van der Maaten M. J., Miller J. M. Conditions for binding bovine IgG1 to protein A-Sepharose. Mol Immunol. 1985 May;22(5):613–616. doi: 10.1016/0161-5890(85)90186-5. [DOI] [PubMed] [Google Scholar]
  8. Terpstra W. J., Korver H., van Leeuwen J., Klatser P. R., Kolk A. H. The classification of Sejroe group serovars of Leptospira interrogans with monoclonal antibodies. Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Jul;259(4):498–506. doi: 10.1016/s0176-6724(85)80082-1. [DOI] [PubMed] [Google Scholar]
  9. Thiermann A. B., Garrett L. A. Enzyme-linked immunosorbent assay for the detection of antibodies to Leptospira interrogans serovars hardjo and pomona in cattle. Am J Vet Res. 1983 May;44(5):884–887. [PubMed] [Google Scholar]
  10. Thiermann A. B., Handsaker A. L., Foley J. W., White F. H., Kingscote B. F. Reclassification of North American leptospiral isolates belonging to serogroups Mini and Sejroe by restriction endonuclease analysis. Am J Vet Res. 1986 Jan;47(1):61–66. [PubMed] [Google Scholar]
  11. Thiermann A. B., Handsaker A. L., Moseley S. L., Kingscote B. New method for classification of leptospiral isolates belonging to serogroup pomona by restriction endonuclease analysis: serovar kennewicki. J Clin Microbiol. 1985 Apr;21(4):585–587. doi: 10.1128/jcm.21.4.585-587.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

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