Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Nov;25(11):2223–2224. doi: 10.1128/jcm.25.11.2223-2224.1987

Rapid identification of pathogenic Neisseria species and Branhamella catarrhalis.

B Hughes 1, M T Pezzlo 1, L M de la Maza 1, E M Peterson 1
PMCID: PMC269449  PMID: 3121668

Abstract

Two systems, the Identicult-Neisseria (IDN; Scott Laboratories, Inc., Fiskeville, R.I.) strip and the Neisseria/Haemophilus Identification Test Kit (NHI; Vitek Systems, Inc., Hazelwood, Mo.) card, were compared with the 4-h Minitek system (BBL Microbiology Systems, Cockeysville, Md.) for their ability to rapidly identify 157 pathogenic Neisseria and Branhamella catarrhalis isolates. IDN, limited in its identification to four species, when incubated at 35 degrees C for 10 min identified 99% of the isolates. However, when IDN was incubated at 22 degrees C for 20 min, it identified only 92% of the isolates. The NHI card, a rapid semiautomated system with the ability to identify 25 organisms to the species level, correctly identified all of the isolates. A test for beta-lactamase production included in the NHI card identified the 12 Neisseria gonorrhoeae and 10 B. catarrhalis beta-lactamase-positive isolates included in the study. The IDN strip (35 degrees C) and the NHI card compared favorably with the Minitek system.

Full text

PDF
2224

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Hosmer M. E., Cohenford M. A., Ellner P. D. Preliminary evaluation of a rapid colorimetric method for identification of pathogenic Neisseria. J Clin Microbiol. 1986 Jul;24(1):141–142. doi: 10.1128/jcm.24.1.141-142.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Philip A., Garton G. C. Comparative evaluation of five commercial systems for the rapid identification of pathogenic Neisseria species. J Clin Microbiol. 1985 Jul;22(1):101–104. doi: 10.1128/jcm.22.1.101-104.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Yajko D. M., Chu A., Hadley W. K. Rapid confirmatory identification of Neisseria gonorrhoeae with lectins and chromogenic substrates. J Clin Microbiol. 1984 Mar;19(3):380–382. doi: 10.1128/jcm.19.3.380-382.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES