Abstract
We evaluated a monoclonal fluorescent-antibody (FA) reagent (Neisseria gonorrhoeae Culture Confirmation Test; Syva Co., Palo Alto, Calif.) for confirmation of N. gonorrhoeae isolates obtained from clinics for sexually transmitted diseases in four cities. The FA test was performed in parallel with established confirmation procedures on all organisms growing on 773 primary culture plates of modified Thayer-Martin agar. All N. gonorrhoeae isolates reacted with the FA reagent and produced a bright, easily interpretable fluorescence. The FA test correctly identified 533 N. gonorrhoeae isolates from 474 patients and did not react with 90 N. meningitidis or with 213 non-Neisseria isolates. In one city (Baltimore), Gonochek II (Du Pont Co., Wilmington, Del.) failed to identify four N. gonorrhoeae isolates reactive with the FA reagent and confirmed as N. gonorrhoeae by Phadebact (Pharmacia Inc., Piscataway, N.J.) and acid production from sugars. The FA test was rapid and specific and could be performed directly from primary isolation plates. The test requires 1 h to perform and is applicable to mixed-flora cultures.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arko R. J., Finley-Price K. G., Wong K. H., Johnson S. R., Reising G. Identification of problem Neisseria gonorrhoeae cultures by standard and experimental tests. J Clin Microbiol. 1982 Mar;15(3):435–438. doi: 10.1128/jcm.15.3.435-438.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dossett J. H., Appelbaum P. C., Knapp J. S., Totten P. A. Proctitis associated with Neisseria cinerea misidentified as Neisseria gonorrhoeae in a child. J Clin Microbiol. 1985 Apr;21(4):575–577. doi: 10.1128/jcm.21.4.575-577.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Griffin C. W., 3rd, Mehaffey M. A., Cook E. C. Five years of experience with a national external quality control program for the culture and identification of Neisseria gonorrhoeae. J Clin Microbiol. 1983 Nov;18(5):1150–1159. doi: 10.1128/jcm.18.5.1150-1159.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janda W. M., Zigler K. L., Bradna J. J. API QuadFERM+ with rapid DNase for identification of Neisseria spp. and Branhamella catarrhalis. J Clin Microbiol. 1987 Feb;25(2):203–206. doi: 10.1128/jcm.25.2.203-206.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morello J. A., Lerner S. A., Bohnhoff M. Characteristics of atypical Neisseria gonorrhoeae from disseminated and localized infections. Infect Immun. 1976 May;13(5):1510–1516. doi: 10.1128/iai.13.5.1510-1516.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Philip A., Garton G. C. Comparative evaluation of five commercial systems for the rapid identification of pathogenic Neisseria species. J Clin Microbiol. 1985 Jul;22(1):101–104. doi: 10.1128/jcm.22.1.101-104.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pollock H. M. Evaluation of methods for the rapid identification of Neisseria gonorrhoeae in a routine clinical laboratory. J Clin Microbiol. 1976 Jul;4(1):19–21. doi: 10.1128/jcm.4.1.19-21.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tam M. R., Buchanan T. M., Sandström E. G., Holmes K. K., Knapp J. S., Siadak A. W., Nowinski R. C. Serological classification of Neisseria gonorrhoeae with monoclonal antibodies. Infect Immun. 1982 Jun;36(3):1042–1053. doi: 10.1128/iai.36.3.1042-1053.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vera H. D. A Simple Medium for Identification and Maintenance of the Gonococcus and Other Bacteria. J Bacteriol. 1948 Apr;55(4):531–536. doi: 10.1128/jb.55.4.531-536.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]