Abstract
Four hundred thirty-two lactose-negative colonies isolated from human feces on stool differential agar media were flooded with one drop of MUCAP Test reagent (Biolife Italiana S.r.I., Milan, Italy) and observed under a Wood lamp for the development of a blue fluorescence over or around the colony. On the basis of manual and automated conventional tests for the screening of Salmonella spp., the MUCAP Test yielded the following results: 79 true-positives, 314 true-negatives, 35 false-positives, and 4 false-negatives (sensitivity, 95%; specificity, 90%; positive predictive value, 69%; negative predictive value, 99%). The specificity of the test performed on colonies isolated on MacConkey agar (95%) was higher than that performed on colonies isolated on SS agar (88%; P less than 0.03). The MUCAP Test is an easy, rapid, and sensitive method for the screening of colonies suspected of being Salmonella spp., reducing the number of biochemical tests needed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Godsey J. H., Matteo M. R., Shen D., Tolman G., Gohlke J. R. Rapid identification of Enterobacteriaceae with microbial enzyme activity profiles. J Clin Microbiol. 1981 Mar;13(3):483–490. doi: 10.1128/jcm.13.3.483-490.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greene L. C., Appelbaum P. C., Kellogg J. A. Evaluation of a two-hour method for screening pathogens from stool specimens. J Clin Microbiol. 1984 Aug;20(2):285–287. doi: 10.1128/jcm.20.2.285-287.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Littel K. J., Hartman P. A. Fluorogenic selective and differential medium for isolation of fecal streptococci. Appl Environ Microbiol. 1983 Feb;45(2):622–627. doi: 10.1128/aem.45.2.622-627.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maddocks J. L., Greenan M. J. A rapid method for identifying bacterial enzymes. J Clin Pathol. 1975 Aug;28(8):686–687. doi: 10.1136/jcp.28.8.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Villasante P. A., Agulla A., Merino F. J., Pérez T., Ladrón de Guevara C., Velasco A. C. Rapid automated method for screening of enteric pathogens from stool specimens. J Clin Microbiol. 1987 Mar;25(3):584–585. doi: 10.1128/jcm.25.3.584-585.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]