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. 1991 Jan;29(1):59–61. doi: 10.1128/jcm.29.1.59-61.1991

Evaluation of the Staph-Zym system with staphylococci isolated from bovine intramammary infections.

J L Watts 1, P J Washburn 1
PMCID: PMC269703  PMID: 1993769

Abstract

A total of 148 staphylococci isolated from bovine intramammary infections were used to evaluate the Staph-Zym system (ROSCO, Taastrup, Denmark). The overall accuracy of the system was 91.9%. The system correctly identified all strains of Staphylococcus aureus, Staphylococcus simulans, and Staphylococcus xylosus and 95% of Staphylococcus intermedius strains. Of 33 Staphylococcus hyicus strains, 31 (93.9%) were classified correctly by the Staph-Zym system, as well as 8 (80%) of 10 Staphylococcus chromogenes strains. All 11 Staphylococcus epidermidis strains and the 1 Staphylococcus haemolyticus strain included in the study were identified, but the Staph-Zym system had difficulty distinguishing strains of Staphylococcus warneri and Staphylococcus hominis from other species in the S. epidermidis group. The Staph-Zym system correctly identified all six S. xylosus strains and two of three Staphyloccus sciuri strains. The Staph-Zym system was considered an acceptable alternative to conventional methods for identification of bovine mammary gland isolates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cox H. U., Newman S. S., Roy A. F., Hoskins J. D. Species of Staphylococcus isolated from animal infections. Cornell Vet. 1984 Apr;74(2):124–135. [PubMed] [Google Scholar]
  2. Devriese L. A., De Keyser H. Prevalence of different species of coagulase-negative staphylococci on teats and in milk samples from dairy cows. J Dairy Res. 1980 Feb;47(1):155–158. doi: 10.1017/s0022029900020999. [DOI] [PubMed] [Google Scholar]
  3. Devriese L. A. Identification of clumping-factor-negative staphylococci isolated from cows' udders. Res Vet Sci. 1979 Nov;27(3):313–320. [PubMed] [Google Scholar]
  4. Devriese L. A., Schleifer K. H., Adegoke G. O. Identification of coagulase-negative staphylococci from farm animals. J Appl Bacteriol. 1985 Jan;58(1):45–55. doi: 10.1111/j.1365-2672.1985.tb01428.x. [DOI] [PubMed] [Google Scholar]
  5. Hogan J. S., White D. G., Pankey J. W. Effects of teat dipping on intramammary infections by staphylococci other than Staphylococcus aureus. J Dairy Sci. 1987 Apr;70(4):873–879. doi: 10.3168/jds.S0022-0302(87)80086-3. [DOI] [PubMed] [Google Scholar]
  6. Kloos W. E. Natural populations of the genus Staphylococcus. Annu Rev Microbiol. 1980;34:559–592. doi: 10.1146/annurev.mi.34.100180.003015. [DOI] [PubMed] [Google Scholar]
  7. Kloos W. E., Schleifer K. H. Simplified scheme for routine identification of human Staphylococcus species. J Clin Microbiol. 1975 Jan;1(1):82–88. doi: 10.1128/jcm.1.1.82-88.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Langlois B. E., Harmon R. J., Akers K. Identification of Staphylococcus species of bovine origin with the API Staph-Ident system. J Clin Microbiol. 1983 Nov;18(5):1212–1219. doi: 10.1128/jcm.18.5.1212-1219.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Langlois B. E., Harmon R. J., Akers K. Identification of Staphylococcus species of bovine origin with the DMS Staph-Trac system. J Clin Microbiol. 1984 Aug;20(2):227–230. doi: 10.1128/jcm.20.2.227-230.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lämmler C. Evaluation of the Staph-Zym-system for identification of Staphylococcus hyicus and Staphylococcus intermedius. Zentralbl Veterinarmed B. 1989 May;36(3):180–184. doi: 10.1111/j.1439-0450.1989.tb00589.x. [DOI] [PubMed] [Google Scholar]
  11. Namavar F., de Graaff J., MacLaren D. M. Taxonomy of coagulase-negative staphylococci: a comparison of two widely used classification schemes. Antonie Van Leeuwenhoek. 1978;44(3-4):425–434. doi: 10.1007/BF00394318. [DOI] [PubMed] [Google Scholar]
  12. Watts J. L., Nickerson S. C. A comparison of the STAPH-Ident and STAPH-Trac systems to conventional methods in the identification of staphylococci isolated from bovine udders. Vet Microbiol. 1986 Jul;12(2):179–187. doi: 10.1016/0378-1135(86)90079-9. [DOI] [PubMed] [Google Scholar]
  13. Watts J. L., Owens W. E., Nickerson S. C. Evaluation of the Minitek gram-positive set for identification of staphylococci isolated from the bovine mammary gland. J Clin Microbiol. 1986 May;23(5):873–875. doi: 10.1128/jcm.23.5.873-875.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Watts J. L., Owens W. E., Nickerson S. C. Identification of staphylococci from bovine udders: evaluation of the API 20GP system. Can J Microbiol. 1986 Apr;32(4):359–361. doi: 10.1139/m86-069. [DOI] [PubMed] [Google Scholar]
  15. Watts J. L., Owens W. E. Prevalence of staphylococcal species in four dairy herds. Res Vet Sci. 1989 Jan;46(1):1–4. [PubMed] [Google Scholar]
  16. Watts J. L., Pankey J. W., Nickerson S. C. Evaluation of the Staph-Ident and STAPHase systems for identification of staphylococci from bovine intramammary infections. J Clin Microbiol. 1984 Sep;20(3):448–452. doi: 10.1128/jcm.20.3.448-452.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. White D. G., Harmon R. J., Langlois B. E. Fluorogenic assay for differentiating Staphylococcus warneri and Staphylococcus hominis strains of bovine origin. J Clin Microbiol. 1990 Mar;28(3):602–602. doi: 10.1128/jcm.28.3.602-.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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