Abstract
The methods and sampling procedures used in the diagnosis of Helicobacter pylori infection and chronic active gastritis were evaluated. Five biopsy specimens for bacteriological cultivation and three specimens for histological examination were obtained endoscopically from a defined area of the gastric antral mucosae of 83 patients. An increase in the number of biopsy specimens for cultivation from one to five revealed only one more H. pylori-infected patient. H. pylori was isolated from 31 of 83 patients. Three technically adequate samples for histological examination were obtained from each of 74 patients. Of these 74 patients, chronic active gastritis was diagnosed by demonstration of typical histological changes in all three specimens from each of 20 patients, in two of three specimens from each of 3 patients, and in one of three specimens from 1 patient. The results indicate that one biopsy specimen is sufficient for the isolation of H. pylori, whereas several specimens may be necessary for the histological diagnosis. Chronic active gastritis was found in four patients not infected with H. pylori; on the other hand, H. pylori was isolated from nine patients who showed no signs of chronic active gastritis in any of three samples.
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- Aase S., Elgjo K., Fausa O., Bjørneklett A., Nedenskov-Sørensen P., Bukholm G. Bacteria of the gastric antrum and their relation to chronic gastritis. APMIS. 1988 Mar;96(3):273–279. doi: 10.1111/j.1699-0463.1988.tb05301.x. [DOI] [PubMed] [Google Scholar]
- Goodwin C. S., Blincow E. D., Warren J. R., Waters T. E., Sanderson C. R., Easton L. Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa. J Clin Pathol. 1985 Oct;38(10):1127–1131. doi: 10.1136/jcp.38.10.1127. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones D. M., Lessells A. M., Eldridge J. Campylobacter like organisms on the gastric mucosa: culture, histological, and serological studies. J Clin Pathol. 1984 Sep;37(9):1002–1006. doi: 10.1136/jcp.37.9.1002. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris A., Ali M. R., Brown P., Lane M., Patton K. Campylobacter pylori infection in biopsy specimens of gastric antrum: laboratory diagnosis and estimation of sampling error. J Clin Pathol. 1989 Jul;42(7):727–732. doi: 10.1136/jcp.42.7.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nedenskov-Sørensen P., Bjørneklett A., Fausa O., Bukholm G., Aase S., Jantzen E. Campylobacter pylori infection and its relation to chronic gastritis. An endoscopic, bacteriologic, and histomorphologic study. Scand J Gastroenterol. 1988 Sep;23(7):867–874. doi: 10.3109/00365528809090775. [DOI] [PubMed] [Google Scholar]
- Taylor D. E., Hargreaves J. A., Ng L. K., Sherbaniuk R. W., Jewell L. D. Isolation and characterization of Campylobacter pyloridis from gastric biopsies. Am J Clin Pathol. 1987 Jan;87(1):49–54. doi: 10.1093/ajcp/87.1.49. [DOI] [PubMed] [Google Scholar]
- Whitehead R., Truelove S. C., Gear M. W. The histological diagnosis of chronic gastritis in fibreoptic gastroscope biopsy specimens. J Clin Pathol. 1972 Jan;25(1):1–11. doi: 10.1136/jcp.25.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]