Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 May;29(5):901–905. doi: 10.1128/jcm.29.5.901-905.1991

Clinical comparison of the recoveries of bloodstream pathogens in Septi-Chek brain heart infusion broth with saponin, Septi-Chek tryptic soy broth, and the isolator lysis-centrifugation system.

P R Murray 1, A W Spizzo 1, A C Niles 1
PMCID: PMC269905  PMID: 2056056

Abstract

The recoveries of microorganisms in the Septi-Chek biphasic tryptic soy broth (TSB) and Isolator blood culturing systems were compared with the recovery in Septi-Chek biphasic brain heart infusion broth supplemented with the lytic agent saponin (L-BHIB; Roche Diagnostics, Division of Hoffmann-La Roche Inc.). A total of 5,096 TSB-L-BHIB blood culture sets and 1,778 Isolator-L-BHIB sets were evaluated. There were 435 pathogenic organisms recovered in 413 (8.1%) of the TSB-L-BHIB cultures, with 348 organisms in the TSB bottles and 370 in the L-BHIB bottles (P less than 0.05). Significantly more yeast isolates were recovered in the L-BHIB system (P less than 0.01). Terminal subculture of the L-BHIB system was required to detect 13 clinically significant organisms. Significantly more organisms were recovered in the L-BHIB bottles than with the Isolator system (P less than 0.001), including Torulopsis glabrata, coagulase-negative staphylococci, and Pseudomonas species. The time required for detection of all major groups of organisms was the same in both comparisons. However, detection of microbial growth in the L-BHIB bottles required macroscopic inspection of the attached agar slide unit and the use of terminal subcultures after 7 days of incubation. The primary advantage of the biphasic L-BHIB system when compared with either the biphasic TSB system or the Isolator system was improved recovery of fungi.

Full text

PDF
902

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bille J., Edson R. S., Roberts G. D. Clinical evaluation of the lysis-centrifugation blood culture system for the detection of fungemia and comparison with a conventional biphasic broth blood culture system. J Clin Microbiol. 1984 Feb;19(2):126–128. doi: 10.1128/jcm.19.2.126-128.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brannon P., Kiehn T. E. Large-scale clinical comparison of the lysis-centrifugation and radiometric systems for blood culture. J Clin Microbiol. 1985 Dec;22(6):951–954. doi: 10.1128/jcm.22.6.951-954.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carey R. B. Clinical comparison of the Isolator 1.5 microbial tube and the BACTEC radiometric system for detection of bacteremia in children. J Clin Microbiol. 1984 May;19(5):634–638. doi: 10.1128/jcm.19.5.634-638.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DeLand F. H., Wagner H. N., Jr Early detection of bacterial growth, with carbon-14-labeled glucose. Radiology. 1969 Jan;92(1):154–155. doi: 10.1148/92.1.154. [DOI] [PubMed] [Google Scholar]
  5. Durbin W. A., Szymczak E. G., Goldmann D. A. Quantitative blood cultures in childhood bacteremia. J Pediatr. 1978 May;92(5):778–780. doi: 10.1016/s0022-3476(78)80151-6. [DOI] [PubMed] [Google Scholar]
  6. Hansen S. L., Hetmanski J., Stewart B. J. Resin-process methods for improved isolation of organisms from blood and other body fluids. Am J Med. 1983 Jul 28;75(1B):31–36. doi: 10.1016/0002-9343(83)90070-0. [DOI] [PubMed] [Google Scholar]
  7. Hawkins B. L., Peterson E. M., de la Maza L. M. Improvement of positive blood culture detection by agitation. Diagn Microbiol Infect Dis. 1986 Sep;5(3):207–213. doi: 10.1016/0732-8893(86)90003-9. [DOI] [PubMed] [Google Scholar]
  8. Henry N. K., Grewell C. M., Van Grevenhof P. E., Ilstrup D. M., Washington J. A., 2nd Comparison of lysis-centrifugation with a biphasic blood culture medium for the recovery of aerobic and facultatively anaerobic bacteria. J Clin Microbiol. 1984 Sep;20(3):413–416. doi: 10.1128/jcm.20.3.413-416.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Henry N. K., McLimans C. A., Wright A. J., Thompson R. L., Wilson W. R., Washington J. A., 2nd Microbiological and clinical evaluation of the isolator lysis-centrifugation blood culture tube. J Clin Microbiol. 1983 May;17(5):864–869. doi: 10.1128/jcm.17.5.864-869.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hopfer R. L., Moore D. G., Fainstein V., Watkins S., Wenglar M. Routine use of BACTEC 16B bottles to remove antibacterial and antitumor agents from blood cultures of cancer patients. J Clin Microbiol. 1983 Oct;18(4):759–764. doi: 10.1128/jcm.18.4.759-764.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ilstrup D. M., Washington J. A., 2nd The importance of volume of blood cultured in the detection of bacteremia and fungemia. Diagn Microbiol Infect Dis. 1983 Jun;1(2):107–110. doi: 10.1016/0732-8893(83)90039-1. [DOI] [PubMed] [Google Scholar]
  12. Kelly M. T., Fojtasek M. F., Abbott T. M., Hale D. C., Dizikes J. R., Boshard R., Buck G. E., Martin W. J., Matsen J. M. Clinical evaluation of a lysis-centrifugation technique for the detection of septicemia. JAMA. 1983 Oct 28;250(16):2185–2188. [PubMed] [Google Scholar]
  13. Kiehn T. E., Wong B., Edwards F. F., Armstrong D. Comparative recovery of bacteria and yeasts from lysis-centrifugation and a conventional blood culture system. J Clin Microbiol. 1983 Aug;18(2):300–304. doi: 10.1128/jcm.18.2.300-304.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kim M. J., Gottschall R. L., Schwabe L. D., Randall E. L. Effect of agitation and frequent subculturing on recovery of aerobic and facultative pathogens by Roche Septi-Chek and BACTEC blood culture systems. J Clin Microbiol. 1987 Feb;25(2):312–315. doi: 10.1128/jcm.25.2.312-315.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McLaughlin J. C., Hamilton P., Scholes J. V., Bartlett R. C. Clinical laboratory comparison of lysis-centrifugation and BACTEC radiometric blood culture techniques. J Clin Microbiol. 1983 Nov;18(5):1027–1031. doi: 10.1128/jcm.18.5.1027-1031.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Murray P. R. Comparison of the lysis-centrifugation and agitated biphasic blood culture systems for detection of fungemia. J Clin Microbiol. 1991 Jan;29(1):96–98. doi: 10.1128/jcm.29.1.96-98.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pfaller M. A., Sibley T. K., Westfall L. M., Hoppe-Bauer J. E., Keating M. A., Murray P. R. Clinical laboratory comparison of a slide blood culture system with a conventional broth system. J Clin Microbiol. 1982 Sep;16(3):525–530. doi: 10.1128/jcm.16.3.525-530.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Plorde J. J., Tenover F. C., Carlson L. G. Specimen volume versus yield in the BACTEC blood culture system. J Clin Microbiol. 1985 Aug;22(2):292–295. doi: 10.1128/jcm.22.2.292-295.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Roberts G. D., Washington J. A., 2nd Detection of fungi in blood cultures. J Clin Microbiol. 1975 Mar;1(3):309–310. doi: 10.1128/jcm.1.3.309-310.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rubin L. G., Baranowski J. Comparison of the Du Pont Isolator 1.5 Microbial Tube and Trypticase soy broth for the recovery of Haemophilus influenzae type b in experimental bacteremia. J Clin Microbiol. 1985 Nov;22(5):815–818. doi: 10.1128/jcm.22.5.815-818.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sullivan N. M., Sutter V. L., Finegold S. M. Practical aerobic membrane filtration blood culture technique: clinical blood culture trial. J Clin Microbiol. 1975 Jan;1(1):37–43. doi: 10.1128/jcm.1.1.37-43.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Thorpe T. C., Wilson M. L., Turner J. E., DiGuiseppi J. L., Willert M., Mirrett S., Reller L. B. BacT/Alert: an automated colorimetric microbial detection system. J Clin Microbiol. 1990 Jul;28(7):1608–1612. doi: 10.1128/jcm.28.7.1608-1612.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weinstein M. P., Mirrett S., Reimer L. G., Reller L. B. Effect of agitation and terminal subcultures on yield and speed of detection of the Oxoid Signal blood culture system versus the BACTEC radiometric system. J Clin Microbiol. 1989 Mar;27(3):427–430. doi: 10.1128/jcm.27.3.427-430.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Weinstein M. P., Reller L. B., Mirrett S., Wang W. L., Alcid D. V. Controlled evaluation of Trypticase soy broth in agar slide and conventional blood culture systems. J Clin Microbiol. 1985 Apr;21(4):626–629. doi: 10.1128/jcm.21.4.626-629.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Welch D. F., Scribner R. K., Hensel D. Evaluation of a lysis direct plating method for pediatric blood cultures. J Clin Microbiol. 1985 Jun;21(6):955–958. doi: 10.1128/jcm.21.6.955-958.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wright A. J., Thompson R. L., McLimans C. A., Wilson W. R., Washington J. A., 2nd The antimicrobial removal device. A microbiological and clinical evaluation. Am J Clin Pathol. 1982 Aug;78(2):173–177. doi: 10.1093/ajcp/78.2.173. [DOI] [PubMed] [Google Scholar]
  27. Zierdt C. H., Peterson D. L., Swan J. C., MacLowry J. D. Lysis-filtration blood culture versus conventional blood culture in a bacteremic rabbit model. J Clin Microbiol. 1982 Jan;15(1):74–77. doi: 10.1128/jcm.15.1.74-77.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES