Abstract
Oocysts of Cryptosporidium parvum were used to infect Madin-Darby bovine kidney cells. Cultures were incubated in a reduced-oxygen atmosphere in candle jars or in a 5% CO2-95% air atmosphere. At 72 h, parasites were quantitated microscopically and found to be enhanced 5.5-fold in the reduced-oxygen atmosphere. Using candle jars, we then determined that C. parvum was amenable to [3H]uracil incorporation assays and easily quantitated with this method.
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- Current W. L., Haynes T. B. Complete development of Cryptosporidium in cell culture. Science. 1984 May 11;224(4649):603–605. doi: 10.1126/science.6710159. [DOI] [PubMed] [Google Scholar]
- Current W. L., Reese N. C. A comparison of endogenous development of three isolates of Cryptosporidium in suckling mice. J Protozool. 1986 Feb;33(1):98–108. doi: 10.1111/j.1550-7408.1986.tb05567.x. [DOI] [PubMed] [Google Scholar]
- Flanigan T. P., Aji T., Marshall R., Soave R., Aikawa M., Kaetzel C. Asexual development of Cryptosporidium parvum within a differentiated human enterocyte cell line. Infect Immun. 1991 Jan;59(1):234–239. doi: 10.1128/iai.59.1.234-239.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jensen J. B., Trager W. Plasmodium falciparum in culture: use of outdated erthrocytes and description of the candle jar method. J Parasitol. 1977 Oct;63(5):883–886. [PubMed] [Google Scholar]
- Kilani R. T., Sekla L. Purification of Cryptosporidium oocysts and sporozoites by cesium chloride and Percoll gradients. Am J Trop Med Hyg. 1987 May;36(3):505–508. doi: 10.4269/ajtmh.1987.36.505. [DOI] [PubMed] [Google Scholar]
- Lumb R., Smith K., O'Donoghue P. J., Lanser J. A. Ultrastructure of the attachment of Cryptosporidium sporozoites to tissue culture cells. Parasitol Res. 1988;74(6):531–536. doi: 10.1007/BF00531630. [DOI] [PubMed] [Google Scholar]
- McDonald V., Stables R., Warhurst D. C., Barer M. R., Blewett D. A., Chapman H. D., Connolly G. M., Chiodini P. L., McAdam K. P. In vitro cultivation of Cryptosporidium parvum and screening for anticryptosporidial drugs. Antimicrob Agents Chemother. 1990 Aug;34(8):1498–1500. doi: 10.1128/aac.34.8.1498. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pfefferkorn E. R., Pfefferkorn L. C. Specific labeling of intracellular Toxoplasma gondii with uracil. J Protozool. 1977 Aug;24(3):449–453. doi: 10.1111/j.1550-7408.1977.tb04774.x. [DOI] [PubMed] [Google Scholar]
- Schmatz D. M., Crane M. S., Murray P. K. Eimeria tenella: parasite-specific incorporation of 3H-uracil as a quantitative measure of intracellular development. J Protozool. 1986 Feb;33(1):109–114. doi: 10.1111/j.1550-7408.1986.tb05568.x. [DOI] [PubMed] [Google Scholar]
- Tilley M., Upton S. J. A comparative study of the development of Eimeria nieschulzi in vitro under aerobic and reducing conditions. J Parasitol. 1988 Dec;74(6):1042–1045. [PubMed] [Google Scholar]
- Tilley M., Upton S. J., Blagburn B. L., Anderson B. C. Identification of outer oocyst wall proteins of three Cryptosporidium (Apicomplexa: Cryptosporidiidae) species by 125I surface labeling. Infect Immun. 1990 Jan;58(1):252–253. doi: 10.1128/iai.58.1.252-253.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trager W., Jensen J. B. Human malaria parasites in continuous culture. Science. 1976 Aug 20;193(4254):673–675. doi: 10.1126/science.781840. [DOI] [PubMed] [Google Scholar]
- Ungar B. L., Gilman R. H., Lanata C. F., Perez-Schael I. Seroepidemiology of Cryptosporidium infection in two Latin American populations. J Infect Dis. 1988 Mar;157(3):551–556. doi: 10.1093/infdis/157.3.551. [DOI] [PubMed] [Google Scholar]
- Ungar B. L., Mulligan M., Nutman T. B. Serologic evidence of Cryptosporidium infection in US volunteers before and during Peace Corps service in Africa. Arch Intern Med. 1989 Apr;149(4):894–897. [PubMed] [Google Scholar]