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. 1991 Jul;29(7):1290–1294. doi: 10.1128/jcm.29.7.1290-1294.1991

Expression in Escherichia coli and sequencing of the coding region for the capsid protein of Dutch maedi-visna virus strain ZZV 1050: application of recombinant protein in enzyme-linked immunosorbent assay for the detection of caprine and ovine lentiviruses.

R G Zanoni 1, I M Nauta 1, U Pauli 1, E Peterhans 1
PMCID: PMC270102  PMID: 1653261

Abstract

Maedi-visna in sheep and caprine arthritis-encephalitis in goats are caused by two closely related and widespread lentiviruses. The infections are characterized by life-long virus persistence and slow induction of antiviral antibodies. The diagnosis is based on the detection of antiviral antibodies. We have used the polymerase chain reaction (PCR) to amplify a part of the gag gene coding for the entire capsid protein and for parts of the matrix and nucleocapsid proteins. Sequencing of the PCR fragment of the Dutch maedi-visna virus strain ZZV 1050 revealed 85 and 92% homology to the DNA and deduced amino acid sequences, respectively, of the distantly related Icelandic visna virus strain 1514. The respective homologies with caprine arthritis-encephalitis virus strain CO were 76 and 80%. The PCR fragment was cloned into pGEX-2T and expressed as a glutathione S-transferase fusion protein. The recombinant protein could be detected on immunoblots by using a monoclonal antibody and polyclonal antisera and was further purified by glutathione-based affinity chromatography. Enzyme-linked immunosorbent assay with purified recombinant fusion protein is shown to be a sensitive and specific diagnostic tool for the detection of lentiviral infection in goats and sheep.

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Selected References

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  1. Adams D. S., Gogolewski R. P., Barbet A. F., Cheevers W. P. Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines. J Gen Virol. 1985 May;66(Pt 5):1139–1143. doi: 10.1099/0022-1317-66-5-1139. [DOI] [PubMed] [Google Scholar]
  2. Adams D. S., Gorham J. R. The gp135 of caprine arthritis encephalitis virus affords greater sensitivity than the p28 in immunodiffusion serology. Res Vet Sci. 1986 Mar;40(2):157–160. [PubMed] [Google Scholar]
  3. Almond N., Page M., Mills K., Jenkins A., Ling C., Thorpe R., Kitchin P., Williams M. The production and purification of PCR-derived recombinant simian immunodeficiency virus p27 gag protein; its use in detecting serological and T-cell responses in macaques. J Virol Methods. 1990 Jun;28(3):305–319. doi: 10.1016/0166-0934(90)90124-x. [DOI] [PubMed] [Google Scholar]
  4. Archambault D., Wang Z. M., Lacal J. C., Gazit A., Yaniv A., Dahlberg J. E., Tronick S. R. Development of an enzyme-linked immunosorbent assay for equine infectious anemia virus detection using recombinant Pr55gag. J Clin Microbiol. 1989 Jun;27(6):1167–1173. doi: 10.1128/jcm.27.6.1167-1173.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burke D. S., Redfield R. R., Putman P., Alexander S. S. Variations in Western blot banding patterns of human T-cell lymphotropic virus type III/lymphadenopathy-associated virus. J Clin Microbiol. 1987 Jan;25(1):81–84. doi: 10.1128/jcm.25.1.81-84.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cheevers W. P., Knowles D. P., McGuire T. C., Cunningham D. R., Adams D. S., Gorham J. R. Chronic disease in goats orally infected with two isolates of the caprine arthritis-encephalitis lentivirus. Lab Invest. 1988 May;58(5):510–517. [PubMed] [Google Scholar]
  7. Cheevers W. P., McGuire T. C. The lentiviruses: maedi/visna, caprine arthritis-encephalitis, and equine infectious anemia. Adv Virus Res. 1988;34:189–215. doi: 10.1016/s0065-3527(08)60518-7. [DOI] [PubMed] [Google Scholar]
  8. Chiang C. S., Grove T., Cooper M., Cuan J., Kowalski A., Parcells K., Tsunokawa M., Rosenberg M., Arcuri E., Franklin S. Development of a confirmatory enzyme-linked immunosorbent assay for HIV-1 antibodies. Clin Chem. 1989 Jun;35(6):946–952. [PubMed] [Google Scholar]
  9. Coackley W., Smith V. W., Houwers D. J. Preparation and evaluation of antigens used in serological tests for caprine syncytial retrovirus antibody in sheep and goat sera. Vet Microbiol. 1984 Oct;9(6):581–586. doi: 10.1016/0378-1135(84)90020-8. [DOI] [PubMed] [Google Scholar]
  10. Crawford T. B., Adams D. S. Caprine arthritis-encephalitis: clinical features and presence of antibody in selected goat populations. J Am Vet Med Assoc. 1981 Apr 1;178(7):713–719. [PubMed] [Google Scholar]
  11. Erlich H. A. Polymerase chain reaction. J Clin Immunol. 1989 Nov;9(6):437–447. doi: 10.1007/BF00918012. [DOI] [PubMed] [Google Scholar]
  12. Gogolewski R. P., Adams D. S., McGuire T. C., Banks K. L., Cheevers W. P. Antigenic cross-reactivity between caprine arthritis-encephalitis, visna and progressive pneumonia viruses involves all virion-associated proteins and glycoproteins. J Gen Virol. 1985 Jun;66(Pt 6):1233–1240. doi: 10.1099/0022-1317-66-6-1233. [DOI] [PubMed] [Google Scholar]
  13. Guatelli J. C., Gingeras T. R., Richman D. D. Nucleic acid amplification in vitro: detection of sequences with low copy numbers and application to diagnosis of human immunodeficiency virus type 1 infection. Clin Microbiol Rev. 1989 Apr;2(2):217–226. doi: 10.1128/cmr.2.2.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Houwers D. J., Nauta I. M. Immunoblot analysis of the antibody response to ovine lentivirus infections. Vet Microbiol. 1989 Feb;19(2):127–139. doi: 10.1016/0378-1135(89)90078-3. [DOI] [PubMed] [Google Scholar]
  15. Houwers D. J., Schaake J., Jr An improved ELISA for the detection of antibodies to ovine and caprine lentiviruses, employing monoclonal antibodies in a one-step assay. J Immunol Methods. 1987 Apr 2;98(1):151–154. doi: 10.1016/0022-1759(87)90449-2. [DOI] [PubMed] [Google Scholar]
  16. Kajikawa O., Lairmore M. D., DeMartini J. C. Analysis of antibody responses to phenotypically distinct lentiviruses. J Clin Microbiol. 1990 Apr;28(4):764–770. doi: 10.1128/jcm.28.4.764-770.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Keohavong P., Thilly W. G. Fidelity of DNA polymerases in DNA amplification. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9253–9257. doi: 10.1073/pnas.86.23.9253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Klein J. R., Martin J., Griffing S., Nathanson N., Gorham J., Shen D. T., Petursson G., Georgsson G., Palsson P. A., Lutley R. Precipitating antibodies in experimental visna and natural progressive pneumonia of sheep. Res Vet Sci. 1985 Mar;38(2):129–133. [PubMed] [Google Scholar]
  19. Knowles D., Jr, Cheevers W., McGuire T., Stem T., Gorham J. Severity of arthritis is predicted by antibody response to gp135 in chronic infection with caprine arthritis-encephalitis virus. J Virol. 1990 May;64(5):2396–2398. doi: 10.1128/jvi.64.5.2396-2398.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Krieg A., Peterhans E. Die caprine Arthritis-Encephalitis in der Schweiz: Epidemiologische und klinische Untersuchungen. Schweiz Arch Tierheilkd. 1990;132(7):345–352. [PubMed] [Google Scholar]
  21. Lecomte C., Pin J. J., De Moerlooze L., Vandenbergh D., Lambert A. F., Pastoret P. P., Chappuis G. ELISA detection of bovine viral diarrhoea virus specific antibodies using recombinant antigen and monoclonal antibodies. Vet Microbiol. 1990 Jun;23(1-4):193–201. doi: 10.1016/0378-1135(90)90149-p. [DOI] [PubMed] [Google Scholar]
  22. Leis J., Baltimore D., Bishop J. M., Coffin J., Fleissner E., Goff S. P., Oroszlan S., Robinson H., Skalka A. M., Temin H. M. Standardized and simplified nomenclature for proteins common to all retroviruses. J Virol. 1988 May;62(5):1808–1809. doi: 10.1128/jvi.62.5.1808-1809.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lepine D. G., Neumann P. W., Frenette S. L., O'Shaughnessy M. V. Evaluation of a human immunodeficiency virus test algorithm utilizing a recombinant protein enzyme immunoassay. J Clin Microbiol. 1990 Jun;28(6):1169–1171. doi: 10.1128/jcm.28.6.1169-1171.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Li W. H., Tanimura M., Sharp P. M. Rates and dates of divergence between AIDS virus nucleotide sequences. Mol Biol Evol. 1988 Jul;5(4):313–330. doi: 10.1093/oxfordjournals.molbev.a040503. [DOI] [PubMed] [Google Scholar]
  25. Lindhardt B. O., Gerstoft J., Hofmann B., Pallesen G., Mathiesen L., Dickmeiss E., Ulrich K. Antibodies against the major core protein p24 of human immunodeficiency virus: relation to immunological, clinical and prognostic findings. Eur J Clin Microbiol Infect Dis. 1989 Jul;8(7):614–619. doi: 10.1007/BF01968139. [DOI] [PubMed] [Google Scholar]
  26. McGuire T. C., Brassfield A. L., Davis W. C., Cheevers W. P. Antigenic and structural variation of the p28 core polypeptide of goat and sheep retroviruses. J Gen Virol. 1987 Aug;68(Pt 8):2259–2263. doi: 10.1099/0022-1317-68-8-2259. [DOI] [PubMed] [Google Scholar]
  27. Narayan O., Cork L. C. Lentiviral diseases of sheep and goats: chronic pneumonia leukoencephalomyelitis and arthritis. Rev Infect Dis. 1985 Jan-Feb;7(1):89–98. doi: 10.1093/clinids/7.1.89. [DOI] [PubMed] [Google Scholar]
  28. Ng V. L., Chiang C. S., Debouck C., McGrath M. S., Grove T. H., Mills J. Reliable confirmation of antibodies to human immunodeficiency virus type 1 (HIV-1) with an enzyme-linked immunoassay using recombinant antigens derived from the HIV-1 gag, pol, and env genes. J Clin Microbiol. 1989 May;27(5):977–982. doi: 10.1128/jcm.27.5.977-982.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Pearson W. R., Lipman D. J. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. doi: 10.1073/pnas.85.8.2444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Pyper J. M., Clements J. E., Gonda M. A., Narayan O. Sequence homology between cloned caprine arthritis encephalitis virus and visna virus, two neurotropic lentiviruses. J Virol. 1986 May;58(2):665–670. doi: 10.1128/jvi.58.2.665-670.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Pyper J. M., Clements J. E., Molineaux S. M., Narayan O. Genetic variation among lentiviruses: homology between visna virus and caprine arthritis-encephalitis virus is confined to the 5' gag-pol region and a small portion of the env gene. J Virol. 1984 Sep;51(3):713–721. doi: 10.1128/jvi.51.3.713-721.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Pétursson G., Pálsson P. A., Georgsson G. Maedi-visna in sheep: host-virus interactions and utilization as a model. Intervirology. 1989;30 (Suppl 1):36–44. doi: 10.1159/000150122. [DOI] [PubMed] [Google Scholar]
  33. Querat G., Audoly G., Sonigo P., Vigne R. Nucleotide sequence analysis of SA-OMVV, a visna-related ovine lentivirus: phylogenetic history of lentiviruses. Virology. 1990 Apr;175(2):434–447. doi: 10.1016/0042-6822(90)90428-t. [DOI] [PubMed] [Google Scholar]
  34. Reid-Sanden F. L., Sumner J. W., Smith J. S., Fekadu M., Shaddock J. H., Bellini W. J. Rabies diagnostic reagents prepared from a rabies N gene recombinant expressed in baculovirus. J Clin Microbiol. 1990 May;28(5):858–863. doi: 10.1128/jcm.28.5.858-863.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Saltarelli M., Querat G., Konings D. A., Vigne R., Clements J. E. Nucleotide sequence and transcriptional analysis of molecular clones of CAEV which generate infectious virus. Virology. 1990 Nov;179(1):347–364. doi: 10.1016/0042-6822(90)90303-9. [DOI] [PubMed] [Google Scholar]
  36. Schmidt G., Amiraian K., Frey H., Stevens R. W., Berns D. S. Densitometric analysis of Western blot (immunoblot) assays for human immunodeficiency virus antibodies and correlation with clinical status. J Clin Microbiol. 1987 Oct;25(10):1993–1998. doi: 10.1128/jcm.25.10.1993-1998.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Siitari H., Turunen P., Schrimsher J., Nunn M. New sensitive and specific assay for human immunodeficiency virus antibodies using labeled recombinant fusion protein and time-resolved fluoroimmunoassay. J Clin Microbiol. 1990 Sep;28(9):2022–2029. doi: 10.1128/jcm.28.9.2022-2029.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Smith D. B., Johnson K. S. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988 Jul 15;67(1):31–40. doi: 10.1016/0378-1119(88)90005-4. [DOI] [PubMed] [Google Scholar]
  39. Sonigo P., Alizon M., Staskus K., Klatzmann D., Cole S., Danos O., Retzel E., Tiollais P., Haase A., Wain-Hobson S. Nucleotide sequence of the visna lentivirus: relationship to the AIDS virus. Cell. 1985 Aug;42(1):369–382. doi: 10.1016/s0092-8674(85)80132-x. [DOI] [PubMed] [Google Scholar]
  40. Zanoni R., Krieg A., Peterhans E. Detection of antibodies to caprine arthritis-encephalitis virus by protein G enzyme-linked immunosorbent assay and immunoblotting. J Clin Microbiol. 1989 Mar;27(3):580–582. doi: 10.1128/jcm.27.3.580-582.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Zanoni R., Pauli U., Peterhans E. Detection of caprine arthritis-encephalitis- and maedi-visna viruses using the polymerase chain reaction. Experientia. 1990 Mar 15;46(3):316–319. doi: 10.1007/BF01951776. [DOI] [PubMed] [Google Scholar]
  42. Zhang H., Scholl R., Browse J., Somerville C. Double stranded DNA sequencing as a choice for DNA sequencing. Nucleic Acids Res. 1988 Feb 11;16(3):1220–1220. doi: 10.1093/nar/16.3.1220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Zwahlen R., Aeschbacher M., Balcer T., Stucki M., Wyder-Walther M., Weiss M., Steck F. Lentivirusinfektionen bei Ziegen mit Carpitis und interstitieller Mastitis. Schweiz Arch Tierheilkd. 1983 May;125(5):281–299. [PubMed] [Google Scholar]

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