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. 1991 Jul;29(7):1328–1332. doi: 10.1128/jcm.29.7.1328-1332.1991

Detection and enumeration of toxin-producing Pasteurella multocida with a colony-blot assay.

T Magyar 1, R B Rimler 1
PMCID: PMC270110  PMID: 1885729

Abstract

Colonies of toxin-producing Pasteurella multocida were detected with peroxidase-labeled monoclonal antibodies by a membrane assay. Examination of the specificity of the assay with 29 P. multocida cultures representing various geographic origins, hosts, and serotypes indicated that the test was specific for toxin-producing strains. No cross-reactions were observed with Bordetella species that can be associated with P. multocida in producing diseases in animals. A single membrane could be used to assay several isolated strains for toxin production or to enumerate toxin-producing colonies in mixed cultures. Toxin-producing P. multocida colonies were detected in primary cultures; hence, the assay appears to have good potential for widespread application with clinical samples.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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