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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Aug;29(8):1677–1683. doi: 10.1128/jcm.29.8.1677-1683.1991

Distinction of deep versus superficial clinical and nonclinical isolates of Trichosporon beigelii by isoenzymes and restriction fragment length polymorphisms of rDNA generated by polymerase chain reaction.

B J Kemker 1, P F Lehmann 1, J W Lee 1, T J Walsh 1
PMCID: PMC270183  PMID: 1684798

Abstract

Fifteen clinical and environmental strains of Trichosporon beigelii were analyzed for similarities by using morphological features, biochemical profiles based on carbon compound assimilation and uric acid utilization, isoenzyme electrophoresis, and restriction fragment length polymorphisms of a segment of genes coding for rRNA expanded with the polymerase chain reaction. The findings suggest that strains that cause invasive disease are distinct from the superficial and the nonclinical isolates and that isolates from the skin and mucosae represent a number of different organisms, including some environmental forms. The study shows that T. beigelii is a complex of genetically distinct organisms and that more than one type is found in clinical samples.

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Selected References

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