Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Aug;29(8):1684–1688. doi: 10.1128/jcm.29.8.1684-1688.1991

Identification of Vibrio vulnificus O serovars with antilipopolysaccharide monoclonal antibody.

S J Martin 1, R J Siebeling 1
PMCID: PMC270184  PMID: 1761690

Abstract

A serotyping scheme for Vibrio vulnificus predicated on the detection of lipopolysaccharide (LPS) antigens is proposed. The serovar O typing scheme used to type V. vulnificus employs polyclonal antisera raised in rabbits immunized with heat-killed whole-cell vaccines. Polyclonal typing sera produced in this manner cross-react with heterologous strains. Affinity purification of polyclonal antisera with LPS affinity columns resolved some of these cross-reactions; however, affinity-purified polyclonal antisera still showed cross-reactions that were nonreciprocal. On the basis of the serological patterns that were obtained with affinity-purified polyclonal antisera, V. vulnificus strains were selected as vaccine strains for production of monoclonal antibody. Spleen cells harvested from BALB/c mice immunized with formalin-killed V. vulnificus cells were fused with SP2/O-Ag 14 myeloma cells. Hybridomas were screened by using LPS and whole-cell enzyme-linked immunosorbent assay to identify clones secreting LPS-specific antibodies. Monoclonal antibodies identified five LPS serological varieties of V. vulnificus and a single serovar each for Vibrio damsela and Vibrio hollisae. No cross-reactions between V. vulnificus and V. hollisae or V. damsela were observed.

Full text

PDF
1688

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams L. B., Henk M. C., Siebeling R. J. Detection of Vibrio cholerae with monoclonal antibodies specific for serovar O1 lipopolysaccharide. J Clin Microbiol. 1988 Sep;26(9):1801–1809. doi: 10.1128/jcm.26.9.1801-1809.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adams L. B., Siebeling R. J. Production of Vibrio cholerae O1 and non-O1 typing sera in rabbits immunized with polysaccharide-protein carrier conjugates. J Clin Microbiol. 1984 Feb;19(2):181–186. doi: 10.1128/jcm.19.2.181-186.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Amako K., Okada K., Miake S. Evidence for the presence of a capsule in Vibrio vulnificus. J Gen Microbiol. 1984 Oct;130(10):2741–2743. doi: 10.1099/00221287-130-10-2741. [DOI] [PubMed] [Google Scholar]
  4. Blake P. A., Merson M. H., Weaver R. E., Hollis D. G., Heublein P. C. Disease caused by a marine Vibrio. Clinical characteristics and epidemiology. N Engl J Med. 1979 Jan 4;300(1):1–5. doi: 10.1056/NEJM197901043000101. [DOI] [PubMed] [Google Scholar]
  5. Blake P. A., Weaver R. E., Hollis D. G. Diseases of humans (other than cholera) caused by vibrios. Annu Rev Microbiol. 1980;34:341–367. doi: 10.1146/annurev.mi.34.100180.002013. [DOI] [PubMed] [Google Scholar]
  6. Ghosh H. K., Bowen T. E. Halophilic vibrios from human tissue infections on the pacific coast of Australia. Pathology. 1980 Jul;12(3):397–402. doi: 10.3109/00313028009077102. [DOI] [PubMed] [Google Scholar]
  7. Kaysner C. A., Abeyta C., Jr, Wekell M. M., DePaola A., Jr, Stott R. F., Leitch J. M. Virulent strains of Vibrio vulnificus isolated from estuaries of the United States West Coast. Appl Environ Microbiol. 1987 Jun;53(6):1349–1351. doi: 10.1128/aem.53.6.1349-1351.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kelly M. T. Effect of temperature and salinity on Vibrio (Beneckea) vulnificus occurrence in a Gulf Coast environment. Appl Environ Microbiol. 1982 Oct;44(4):820–824. doi: 10.1128/aem.44.4.820-824.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kreger A. S., Gray L. D., Testa J. Protection of mice against Vibrio vulnificus disease by vaccination with surface antigen preparations and anti-surface antigen antisera. Infect Immun. 1984 Sep;45(3):537–543. doi: 10.1128/iai.45.3.537-543.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kreger A., DeChatelet L., Shirley P. Interaction of Vibrio vulnificus with human polymorphonuclear leukocytes: association of virulence with resistance to phagocytosis. J Infect Dis. 1981 Sep;144(3):244–248. doi: 10.1093/infdis/144.3.244. [DOI] [PubMed] [Google Scholar]
  11. Matsuo T., Kohno S., Ikeda T., Saruwatari K., Ninomiya H. Fulminating lactose-positive Vibrio septicemia. Acta Pathol Jpn. 1978 Nov;28(6):937–948. doi: 10.1111/j.1440-1827.1978.tb01282.x. [DOI] [PubMed] [Google Scholar]
  12. Mertens A., Nagler J., Hansen W., Gepts-Friedenreich E. Halophilic, lactose-positive Vibrio in a case of fatal septicemia. J Clin Microbiol. 1979 Feb;9(2):233–235. doi: 10.1128/jcm.9.2.233-235.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Oliver J. D., Warner R. A., Cleland D. R. Distribution and ecology of Vibrio vulnificus and other lactose-fermenting marine vibrios in coastal waters of the southeastern United States. Appl Environ Microbiol. 1982 Dec;44(6):1404–1414. doi: 10.1128/aem.44.6.1404-1414.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oliver J. D., Warner R. A., Cleland D. R. Distribution of Vibrio vulnificus and other lactose-fermenting vibrios in the marine environment. Appl Environ Microbiol. 1983 Mar;45(3):985–998. doi: 10.1128/aem.45.3.985-998.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shimada T., Sakazaki R. On the serology of Vibrio vulnificus. Jpn J Med Sci Biol. 1984 Oct-Dec;37(5-6):241–246. doi: 10.7883/yoken1952.37.241. [DOI] [PubMed] [Google Scholar]
  16. Simonson J. G., Siebeling R. J. Coagglutination of Vibrio cholerae, Vibrio mimicus, and Vibrio vulnificus with anti-flagellar monoclonal antibody. J Clin Microbiol. 1988 Oct;26(10):1962–1966. doi: 10.1128/jcm.26.10.1962-1966.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Simonson J., Siebeling R. J. Rapid serological identification of Vibrio vulnificus by anti-H coagglutination. Appl Environ Microbiol. 1986 Dec;52(6):1299–1304. doi: 10.1128/aem.52.6.1299-1304.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tassin M. G., Siebeling R. J., Roberts N. C., Larson A. D. Presumptive identification of Vibrio species with H antiserum. J Clin Microbiol. 1983 Aug;18(2):400–407. doi: 10.1128/jcm.18.2.400-407.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wright A. C., Simpson L. M., Oliver J. D., Morris J. G., Jr Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus. Infect Immun. 1990 Jun;58(6):1769–1773. doi: 10.1128/iai.58.6.1769-1773.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yoshida S., Ogawa M., Mizuguchi Y. Relation of capsular materials and colony opacity to virulence of Vibrio vulnificus. Infect Immun. 1985 Feb;47(2):446–451. doi: 10.1128/iai.47.2.446-451.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES