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. 1991 Sep;29(9):2047–2050. doi: 10.1128/jcm.29.9.2047-2050.1991

Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera.

M Musiani 1, M Zerbini 1, D Gibellini 1, G Gentilomi 1, S Venturoli 1, G Gallinella 1, E Ferri 1, S Girotti 1
PMCID: PMC270258  PMID: 1774333

Abstract

A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane substrate. The emitted photons were detected with instant photographic films. In the search for B19 parvovirus DNA, 2,808 serum samples were analyzed.

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Selected References

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  1. Anand A., Gray E. S., Brown T., Clewley J. P., Cohen B. J. Human parvovirus infection in pregnancy and hydrops fetalis. N Engl J Med. 1987 Jan 22;316(4):183–186. doi: 10.1056/NEJM198701223160403. [DOI] [PubMed] [Google Scholar]
  2. Anderson M. J., Jones S. E., Minson A. C. Diagnosis of human parvovirus infection by dot-blot hybridization using cloned viral DNA. J Med Virol. 1985 Feb;15(2):163–172. doi: 10.1002/jmv.1890150209. [DOI] [PubMed] [Google Scholar]
  3. Anderson M. J., Khousam M. N., Maxwell D. J., Gould S. J., Happerfield L. C., Smith W. J. Human parvovirus B19 and hydrops fetalis. Lancet. 1988 Mar 5;1(8584):535–535. doi: 10.1016/s0140-6736(88)91331-1. [DOI] [PubMed] [Google Scholar]
  4. Azzi A., Zakrzewska K., Gentilomi G., Musiani M., Zerbini M. Detection of B19 parvovirus infections by a dot-blot hybridization assay using a digoxigenin-labelled probe. J Virol Methods. 1990 Feb;27(2):125–133. doi: 10.1016/0166-0934(90)90129-4. [DOI] [PubMed] [Google Scholar]
  5. Banerjee D., Pettit S. Endogenous avidin-binding activity in human lymphoid tissue. J Clin Pathol. 1984 Feb;37(2):223–225. doi: 10.1136/jcp.37.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bronstein I., Edwards B., Voyta J. C. 1,2-dioxetanes: novel chemiluminescent enzyme substrates. Applications to immunoassays. J Biolumin Chemilumin. 1989 Jul;4(1):99–111. doi: 10.1002/bio.1170040116. [DOI] [PubMed] [Google Scholar]
  7. Bronstein I., Voyta J. C., Edwards B. A comparison of chemiluminescent and colorimetric substrates in a hepatitis B virus DNA hybridization assay. Anal Biochem. 1989 Jul;180(1):95–98. doi: 10.1016/0003-2697(89)90093-6. [DOI] [PubMed] [Google Scholar]
  8. Bronstein I., Voyta J. C., Thorpe G. H., Kricka L. J., Armstrong G. Chemiluminescent assay of alkaline phosphatase applied in an ultrasensitive enzyme immunoassay of thyrotropin. Clin Chem. 1989 Jul;35(7):1441–1446. [PubMed] [Google Scholar]
  9. Brown T., Anand A., Ritchie L. D., Clewley J. P., Reid T. M. Intrauterine parvovirus infection associated with hydrops fetalis. Lancet. 1984 Nov 3;2(8410):1033–1034. doi: 10.1016/s0140-6736(84)91126-7. [DOI] [PubMed] [Google Scholar]
  10. Chrystie I. L., Almeida J. D., Welch J. Electron microscopic detection of human parvovirus (B19) in a patient with HIV infection. J Med Virol. 1990 Apr;30(4):249–252. doi: 10.1002/jmv.1890300404. [DOI] [PubMed] [Google Scholar]
  11. Clewley J. P. Detection of human parvovirus using a molecularly cloned probe. J Med Virol. 1985 Feb;15(2):173–181. doi: 10.1002/jmv.1890150210. [DOI] [PubMed] [Google Scholar]
  12. Cohen B. J., Buckley M. M., Clewley J. P., Jones V. E., Puttick A. H., Jacoby R. K. Human parvovirus infection in early rheumatoid and inflammatory arthritis. Ann Rheum Dis. 1986 Oct;45(10):832–838. doi: 10.1136/ard.45.10.832. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Cohen B. J., Mortimer P. P., Pereira M. S. Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV). J Hyg (Lond) 1983 Aug;91(1):113–130. doi: 10.1017/s0022172400060095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Cossart Y. E., Field A. M., Cant B., Widdows D. Parvovirus-like particles in human sera. Lancet. 1975 Jan 11;1(7898):72–73. doi: 10.1016/s0140-6736(75)91074-0. [DOI] [PubMed] [Google Scholar]
  15. Cunningham D. A., Pattison J. R., Craig R. K. Detection of parvovirus DNA in human serum using biotinylated RNA hybridisation probes. J Virol Methods. 1988 Mar-Apr;19(3-4):279–288. doi: 10.1016/0166-0934(88)90022-5. [DOI] [PubMed] [Google Scholar]
  16. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  17. Gentilomi G., Musiani M., Zerbini M., Gallinella G., Gibellini D., La Placa M. A hybrido-immunocytochemical assay for the in situ detection of cytomegalovirus DNA using digoxigenin-labeled probes. J Immunol Methods. 1989 Dec 20;125(1-2):177–183. doi: 10.1016/0022-1759(89)90091-4. [DOI] [PubMed] [Google Scholar]
  18. Girotti S., Grigolo B., Ferri E., Ghini S., Carrea G., Bovara R., Roda A., Motta R., Petilino R. Bioluminescent flow sensor for the determination of L-(+)-lactate. Analyst. 1990 Jul;115(7):889–894. doi: 10.1039/an9901500889. [DOI] [PubMed] [Google Scholar]
  19. Heggeness M. H. Avidin binds to condensed chromatin. Stain Technol. 1977 May;52(3):165–169. doi: 10.3109/10520297709116769. [DOI] [PubMed] [Google Scholar]
  20. Kimpton C. P., Corbitt G., Morris D. J. Detection of cytomegalovirus DNA using probes labelled with digoxigenin. J Virol Methods. 1989 Jun;24(3):335–346. doi: 10.1016/0166-0934(89)90046-3. [DOI] [PubMed] [Google Scholar]
  21. Kurtzman G. J., Cohen B., Meyers P., Amunullah A., Young N. S. Persistent B19 parvovirus infection as a cause of severe chronic anaemia in children with acute lymphocytic leukaemia. Lancet. 1988 Nov 19;2(8621):1159–1162. doi: 10.1016/s0140-6736(88)90233-4. [DOI] [PubMed] [Google Scholar]
  22. Mori J., Field A. M., Clewley J. P., Cohen B. J. Dot blot hybridization assay of B19 virus DNA in clinical specimens. J Clin Microbiol. 1989 Mar;27(3):459–464. doi: 10.1128/jcm.27.3.459-464.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Musiani M., Gentilomi G., Zerbini M., Gibellini D., Gallinella G., Pileri S., Baglioni P., La Placa M. In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay. Histochemistry. 1990;94(1):21–25. doi: 10.1007/BF00266785. [DOI] [PubMed] [Google Scholar]
  24. Musiani M., Zerbini M., Gentilomi G., Gallinella G., Venturoli S., Gibellini D., La Placa M. Rapid detection of cytomegalovirus DNA in urine samples with a dot blot hybridization immunoenzymatic assay. J Clin Microbiol. 1990 Sep;28(9):2101–2103. doi: 10.1128/jcm.28.9.2101-2103.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Plummer F. A., Hammond G. W., Forward K., Sekla L., Thompson L. M., Jones S. E., Kidd I. M., Anderson M. J. An erythema infectiosum-like illness caused by human parvovirus infection. N Engl J Med. 1985 Jul 11;313(2):74–79. doi: 10.1056/NEJM198507113130203. [DOI] [PubMed] [Google Scholar]
  26. Roda A., Girotti S., Ghini S., Carrea G. Coupled reactions for the determination of analytes and enzymes based on the use of luminescence. J Biolumin Chemilumin. 1989 Jul;4(1):423–435. doi: 10.1002/bio.1170040157. [DOI] [PubMed] [Google Scholar]
  27. Saarinen U. M., Chorba T. L., Tattersall P., Young N. S., Anderson L. J., Palmer E., Coccia P. F. Human parvovirus B19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia. Blood. 1986 May;67(5):1411–1417. [PubMed] [Google Scholar]
  28. Schaap A. P., Akhavan H., Romano L. J. Chemiluminescent substrates for alkaline phosphatase: application to ultrasensitive enzyme-linked immunoassays and DNA probes. Clin Chem. 1989 Sep;35(9):1863–1864. [PubMed] [Google Scholar]
  29. Serjeant G. R., Topley J. M., Mason K., Serjeant B. E., Pattison J. R., Jones S. E., Mohamed R. Outbreak of aplastic crises in sickle cell anaemia associated with parvovirus-like agent. Lancet. 1981 Sep 19;2(8247):595–597. doi: 10.1016/s0140-6736(81)92739-2. [DOI] [PubMed] [Google Scholar]
  30. Weiland H. T., Salimans M. M., Fibbe W. E., Kluin P. M., Cohen B. J. Prolonged parvovirus B19 infection with severe anaemia in a bone marrow transplant patient. Br J Haematol. 1989 Feb;71(2):300–300. doi: 10.1111/j.1365-2141.1989.tb04276.x. [DOI] [PubMed] [Google Scholar]
  31. Wood G. S., Warnke R. Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem. 1981 Oct;29(10):1196–1204. doi: 10.1177/29.10.7028859. [DOI] [PubMed] [Google Scholar]
  32. Zerbini M., Musiani M., Venturoli S., Gallinella G., Gibellini D., Gentilomi G., La Placa M. Rapid screening for B19 parvovirus DNA in clinical specimens with a digoxigenin-labeled DNA hybridization probe. J Clin Microbiol. 1990 Nov;28(11):2496–2499. doi: 10.1128/jcm.28.11.2496-2499.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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