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. 1991 Oct;29(10):2313–2321. doi: 10.1128/jcm.29.10.2313-2321.1991

Sensitive analysis of genetic heterogeneity of adenovirus types 3 and 7 in the Soviet Union.

G I Golovina 1, F N Zolotaryov 1, T I Yurlova 1
PMCID: PMC270319  PMID: 1658038

Abstract

An analysis of adenovirus strains isolated in the Soviet Union from 1976 to 1988 revealed four genome types of adenovirus type 3 (Ad3), i.e., Ad3a4, Ad3a9, Ad3a10, and Ad3a11, and four genome types of adenovirus type 7 (Ad7), i.e., Ad7p, Ad7a, Ad7a(1-5), and Ad7f1, identified with the DNA restriction enzymes BamHI, BglII, and HindIII. Three of them, Ad3a10, Ad3a11, and Ad7f1, are newly discovered. The genetic heterogeneity of adenoviruses was examined with restriction endonuclease Cfr13I with a 4-base recognition cleavage site. Eighteen different restriction patterns were identified among 21 selected Ad3 strains after cleavage of DNA with Cfr13I. Eight different subtypes were identified among 20 Ad7 strains by the same technique. For estimation of the relationships among these genome subtypes, pairwise analyses of comigrating DNA restriction fragments from isolates of Ad3 and Ad7 were done after digestion with Cfr13I or with restriction endonucleases recognizing DNA sequences of 6 bp. Surprisingly, the results were very discrepant.

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Selected References

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  1. Adrian T., Becker M., Hierholzer J. C., Wigand R. Molecular epidemiology and restriction site mapping of adenovirus 7 genome types. Arch Virol. 1989;106(1-2):73–84. doi: 10.1007/BF01311039. [DOI] [PubMed] [Google Scholar]
  2. Adrian T., Best B., Hierholzer J. C., Wigand R. Molecular epidemiology and restriction site mapping of adenovirus type 3 genome types. J Clin Microbiol. 1989 Jun;27(6):1329–1334. doi: 10.1128/jcm.27.6.1329-1334.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Adrian T., Wigand R. Adenovirus 3-7, an intermediate strain of subgenus B. Intervirology. 1986;26(4):202–206. doi: 10.1159/000149702. [DOI] [PubMed] [Google Scholar]
  4. Arens M., Dilworth V. Remarkably homogeneous population of adenovirus type 3 and 7 genome types. J Clin Microbiol. 1988 Aug;26(8):1604–1608. doi: 10.1128/jcm.26.8.1604-1608.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bailey A. S., Richmond S. J. Genetic heterogeneity of recent isolates of adenovirus types 3, 4, and 7. J Clin Microbiol. 1986 Jul;24(1):30–35. doi: 10.1128/jcm.24.1.30-35.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Drouin J. Cloning of human mitochondrial DNA in Escherichia coli. J Mol Biol. 1980 Jun 15;140(1):15–34. doi: 10.1016/0022-2836(80)90354-x. [DOI] [PubMed] [Google Scholar]
  7. Hassell J. A., Weber J. Genetic analysis of adenovirus type 2. VIII. Physical locations of temperature-sensitive mutations. J Virol. 1978 Dec;28(3):671–678. doi: 10.1128/jvi.28.3.671-678.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Larsen P. L., Tibbetts C. Spontaneous reiterations of DNA sequences near the ends of adenovirus type 3 genomes. Virology. 1985 Nov;147(1):187–200. doi: 10.1016/0042-6822(85)90238-7. [DOI] [PubMed] [Google Scholar]
  9. Li Q. G., Wadell G. Analysis of 15 different genome types of adenovirus type 7 isolated on five continents. J Virol. 1986 Oct;60(1):331–335. doi: 10.1128/jvi.60.1.331-335.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Li Q. G., Wadell G. Comparison of 17 genome types of adenovirus type 3 identified among strains recovered from six continents. J Clin Microbiol. 1988 May;26(5):1009–1015. doi: 10.1128/jcm.26.5.1009-1015.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lutter L. C. Precise location of DNase I cutting sites in the nucleosome core determined by high resolution gel electrophoresis. Nucleic Acids Res. 1979 Jan;6(1):41–56. doi: 10.1093/nar/6.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mautner V., Mackay N. Recombination in adenovirus: analysis of crossover sites in intertypic overlap recombinants. Virology. 1984 Nov;139(1):43–52. doi: 10.1016/0042-6822(84)90328-3. [DOI] [PubMed] [Google Scholar]
  13. O'Donnell B., Bell E., Payne S. B., Mautner V., Desselberger U. Genome analysis of species 3 adenoviruses isolated during summer outbreaks of conjunctivitis and pharyngoconjunctival fever in the Glasgow and London areas in 1981. J Med Virol. 1986 Mar;18(3):213–227. doi: 10.1002/jmv.1890180303. [DOI] [PubMed] [Google Scholar]
  14. Robinson C. C., Tibbetts C. Polar encapsidation of adenovirus DNA: evolutionary variants reveal dispensable sequences near the left ends of Ad3 genomes. Virology. 1984 Sep;137(2):276–286. doi: 10.1016/0042-6822(84)90219-8. [DOI] [PubMed] [Google Scholar]
  15. Spaeren U., Waldeck W., Eliasson R. Hybridization method for quantitation of replicating polyoma DNA sequences. Anal Biochem. 1979 Jul 15;96(2):378–383. doi: 10.1016/0003-2697(79)90595-5. [DOI] [PubMed] [Google Scholar]
  16. Wadell G., Cooney M. K., da Costa Linhares A., de Silva L., Kennett M. L., Kono R., Gui-Fang R., Lindman K., Nascimento J. P., Schoub B. D. Molecular epidemiology of adenoviruses: global distribution of adenovirus 7 genome types. J Clin Microbiol. 1985 Mar;21(3):403–408. doi: 10.1128/jcm.21.3.403-408.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wadell G. Molecular epidemiology of human adenoviruses. Curr Top Microbiol Immunol. 1984;110:191–220. doi: 10.1007/978-3-642-46494-2_7. [DOI] [PubMed] [Google Scholar]

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