Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Oct;29(10):2345–2347. doi: 10.1128/jcm.29.10.2345-2347.1991

Detection and differentiation of antibodies to human T-cell lymphotropic virus types I and II by the immunofluorescence method.

D Gallo 1, L M Penning 1, C V Hanson 1
PMCID: PMC270328  PMID: 1939596

Abstract

We compared the sensitivities of the prototype human T-cell lymphotropic virus type I (HTLV-I)- and HTLV-II-transformed cell lines, MT2 and Mo-T, with that of an HTLV-II-infected cell line, clone 19, established in our laboratory, in the immunofluorescence (IF) test for detection of antibody to HTLV-I and HTLV-II. In addition, IF antibody titers with the three antigens were determined, and the results were compared with HTLV-I and HTLV-II typing by polymerase chain reaction (PCR). The MT2 cell line was more sensitive than the two HTLV-II cell lines for detecting HTLV-I antibody by IF, and clone 19 was more sensitive than Mo-T or MT2 for measuring HTLV-II antibody. In the titration study, the antigen that gave the highest titer correlated completely with the HTLV type determined by PCR, indicating that the relatively simple IF titration method can be used for differentiating HTLV-I and HTLV-II antibody in sera and plasmas.

Full text

PDF
2345

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chen Y. M., Lee T. H., Wiktor S. Z., Shaw G. M., Murphy E. L., Blattner W. A., Essex M. Type-specific antigens for serological discrimination of HTLV-I and HTLV-II infection. Lancet. 1990 Nov 10;336(8724):1153–1155. doi: 10.1016/0140-6736(90)92769-e. [DOI] [PubMed] [Google Scholar]
  2. Gallo D., Diggs J. L., Hanson C. V. Comparison of Western immunoblot antigens and interpretive criteria for detection of antibody to human T-lymphotropic virus types I and II. J Clin Microbiol. 1990 Sep;28(9):2045–2050. doi: 10.1128/jcm.28.9.2045-2050.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gallo D., Hoffman M. N., Cossen C. K., Diggs J. L., Hurst J. W., Penning L. M. Comparison of immunofluorescence, enzyme immunoassay, and Western blot (immunoblot) methods for detection of antibody to human T-cell leukemia virus type I. J Clin Microbiol. 1988 Aug;26(8):1487–1491. doi: 10.1128/jcm.26.8.1487-1491.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kwok S., Gallo D., Hanson C., McKinney N., Poiesz B., Sninsky J. J. High prevalence of HTLV-II among intravenous drug abusers: PCR confirmation and typing. AIDS Res Hum Retroviruses. 1990 Apr;6(4):561–565. doi: 10.1089/aid.1990.6.561. [DOI] [PubMed] [Google Scholar]
  5. Lipka J. J., Bui K., Reyes G. R., Moeckli R., Wiktor S. Z., Blattner W. A., Murphy E. L., Shaw G. M., Hanson C. V., Sninsky J. J. Determination of a unique and immunodominant epitope of human T cell lymphotropic virus type I. J Infect Dis. 1990 Aug;162(2):353–357. doi: 10.1093/infdis/162.2.353. [DOI] [PubMed] [Google Scholar]
  6. Wiktor S. Z., Alexander S. S., Shaw G. M., Weiss S. H., Murphy E. L., Wilks R. J., Shortly V. J., Hanchard B., Blattner W. A. Distinguishing between HTLV-I and HTLV-II by western blot. Lancet. 1990 Jun 23;335(8704):1533–1533. doi: 10.1016/0140-6736(90)93078-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES